00.00-00.00 PROVA
Hall U6 - 20 first floor Hall U6 - 23 first floor Hall U6 - 24 first floor Hall U6 - 25 first floor Hall U6 - 21 first floor Hall U6 - 26 first floor Hall U6 - 27 first floor Hall U6 - 28 first floor
08.30-09.30 Registration
09.30-11.00

09.30-11.00

Short course 1

09.30-11.00

Short course 2

09.30-11.00

Short course 3

09.30-11.00

Short course 4

09.30-11.00

Short course 5

09.30-11.00

Short course 6

09.30-11.00

Short course 7

09.30-11.00

Short course 8

11.00-11.30 Coffee break
11.30-13.00

11.30-13.00

Short course 9

11.30-13.00

Short course 10

11.30-13.00

Short course 11

11.30-13.00

Short course 12

11.30-13.00

Short course 13

11.30-13.00

Short course 14

11.30-13.00

Short course 15

11.30-13.00

Short course 16

   
16.00-19.00

Milan Conservatorio "Giuseppe Verdi"

16.00-19.00 Opening Ceremony

16.00-16.30 Welcome - Chairs of HPLC 2019 Italy

16.30-17.30 Award Presentation

17.30-18.30 "Music for Leonardo" M. Kemp, Oxford University, UK
and young musicians of the "Conservatorio della Musica Giuseppe Verdi di Milano"
directed by maestri M. Baggio and S. Mormone

18.30-19.00 "The art of chromatography" Attila Felinger, University of Pécs, Hungary

19.00-22.00 Welcome Reception
  • Hall U6 - 20 first floor
    08.30-09.30 Registration
    09.30-11.00

    Short course 1

     

    Short course 1

    Introduction of GLP regulations and bioanalytical method validation by LC-M

    Speaker: Perry Wang
    LC-MS Technical Expert

    Biography

    Good laboratory practice (GLP) is a set of regulations intended to assure the quality and integrity of non-clinical laboratory studies that are intended to support research or marketing permits for products regulated by government agencies. The term GLP is most commonly associated with the pharmaceutical industry and the required non-clinical animal testing that must be performed prior to approval of new drug products.

    "Guidance for Industry - Bioanalytical Method Validation" published in May 2018 provides general recommendations for bioanalytical method validation using advanced technologies. This short course will focus on GLP regulations and the bioanalytical method validation for drugs and metabolites in biological matrices using LC-MS. It will help audiences to comply with the regulations for drug discovery and development in the pharmaceutical industry and CROs. The course will also reflect the recently published white papers with regard to bioanalytical method validation using LC-MS.

    This course will benefit the analytical chemists, lab supervisors, QA/QC managers, GLP auditors and CRO consultants who work in the GLP-regulated labs and the pharmaceutical industry. This course will also benefit all levels of management as a refresher course to stay current with the GLP regulations.

    11.00-11.30 Coffee break
    11.30-13.00

    Short course 9

     

    Short course 9

    Drug quality fundamentals: quality control of small molecule drugs

    Speaker: Michael Dong
    MWD Consulting, Norwalk, Connecticut, USA

    Biography

    This introductory short course provides an overview of key drug quality concepts, standards, regulations, and practices in non-clinical development and manufacturing for the pharmaceutical industries. You will learn the roles of quality assurance (QA), quality control (QC), and the chemistry, manufacturing, and control (CMC) drug development process in ensuring the safety, efficacy, and quality of drug products. It covers industry-standard processes such as SOPs, system and method qualification/validation, release testing, specifications, and certificates of analysis (COA). Specific quality attributes and analytical methodologies for drug substances and products in addition to the role of regulatory agencies are described.

    1. Introduction to Drug Quality and CMC quality assurance process
      • What is drug quality? Common quality issues of drug products
      • Important quality attributes: Appearance, form, identity, potency, purity, chirality, uniformity, bioavailability, stability, microbial activity, and sterility.
      • The CMC (Chemistry, Manufacturing and Control) Process in new drug development
      • GLP, GMP, GCP, ICH guidelines and other public standards
      • Regulatory oversight, registrations (IND, NDA, CTA), internal quality systems and processes, QC / QA unit, specifications, Critical Quality Attributes (CQA).
    2. QC of Small Molecule drug substance and product: specifications, release testing, COA, impurities, and stability
      • A case study of a small molecule drug: specifications, release testing, and COA
      • API: physical, appearance, solid state properties, form, chemical (id, MW, structure, purity, chiral purity, potency), moisture, heavy metals, ROI, residual solvents, solubility.
      • Drug products: physical, chemical, performance (dissolution), uniformity, microbial testing.
      • Stability protocols and shelf life determination.
  • Hall U6 - 23 first floor
    08.30-09.30 Registration
    09.30-11.00

    Short course 2

     

    Short course 2

    Green sample preparation for new generation of analytical chemists: new concepts and fundamentals

    Speaker: Janusz Pawliszyn
    University of Waterloo, Waterloo, Canada

    Biography

    In this course several microextraction technologies will be discussed, namely solid phase microextraction (SPME), thin film microextraction (TFME) and needle trap devices (NT). The fundamental principles of these techniques, different calibration methods, coupling strategies to GC, LC, and MS and advantages and disadvantages of each microextraction approach will be covered. In addition, during this short course, recent advances, developments and applications of each technique in fields such as forensic, environmental, food and flavor, and clinical analysis will be described including high-throughput formats compatible with LC and direct coupling to MS for screening applications. Participants interested in clinical and medical applications should consider to take course "BioSPME in clinical, pharmaceutical and biotechnological research" offered in the afternoon.

    The course is targeted at HPLC 2019 participants, who wish to gain a deeper insight into the green sample preparation technologies:

    • PhD students
    • Analytical chemists
    • Scientists interested in on-site screening
    • Laboratory supervisors
    • Scientists and industry regulators
    11.00-11.30 Coffee break
    11.30-13.00

    Short course 10

     

    Short course 10

    BioSPME in clinical, pharmaceutical and biotechnological research

    Speaker: Barbara Bojko
    Nicolaus Copernicus University, Torun, Poland

    Biography

    In the course participants will learn about applications of solid phase microextraction to medical research, clinical practice and drug discovery and development. The high throughput and automation for in vitro and ex vivo study of large as well as small sample volumes i.e. spot sampling, cell line study will be discussed. The course will underline how users can benefit from certain features of various device geometries, shapes, sizes and coatings when developing methods for different applications. Low invasive in vivo and in situ organ sampling in animals and in humans in the view of pharmacokinetics, therapeutic drug monitoring and biomarkers discovery will be explained on several examples. Practical approaches to quantitation with the use of kinetic and equilibrium-based calibration will be clarified. Also, recent advances in untargeted metabolomics and lipidomics will be shown with particular focus on applications to oncology, neuroscience and transplantology. The participants not familiar with SPME technology should consider to register also at course "Green sample preparation for new generation of analytical chemists: new concepts and fundamentals " offered in the morning.

    The course is targeted at both future and current SPME users, who wish to gain a deeper insight into the technology utilized in bioanalysis and thus improve data quality and increase their productivity.

    • PhD students
    • Analytical chemists
    • Biologists, clinicians and bioanalytical scientists
    • Laboratory supervisors
    • Scientists and industry regulators
  • Hall U6 - 24 first floor
    08.30-09.30 Registration
    09.30-11.00

    Short course 3

     

    Short course 3

    Enantioselective "chiral" chromatography: from methodological concepts to real world applications

    Speaker: Wolfgang Lindner
    University of Vienna, Vienna, Austria

    Biography

    Speaker: Michael Lämmerhofer
    University of Tuebingen, Tuebingen, Germany

    Biography

    The aim of this Short Course is to discuss first the background and the general concepts of separating enantiomers via liquid chromatography with focus on the direct enantiomer separation technology. The basic principle relies on the formation of diastereomeric intermediate associates between a chiral selector-moiety (the SO) and the enantiomeric analytes, the selectands (R)- and (S)-SAs. Conceptually the chemically very diversified chiral selector units get either strongly adsorbed or chemically immobilized onto the surface of a support material, most of the time silica-based particles with a controlled porosity. Such a material represents the chiral stationary phase (CSP) which gets then packed into columns leading to the so-called "chiral" columns.
    The formerly written diastereomeric [SO-(R)-SA] and [SO-(S)-SA] associates are physico-chemically different from each other in contrast to the non-associated (R)-SA and (S)-SA enantiomers. The binding strength of these two associates can be described by ΔG(R) and ΔG(S) values which are affected not only by the molecular structure and the steric motifs of the SO and of the SA moieties but also of the type of solvents and additives representing the mobile phase (MP) of the chromatographic system. In reality one can use normal phase (NP) polar organic (PO) or reversed phase (RP) conditions. In addition also SFC type MP compositions can be well applied. With other words, these chromatographic systems represent a "Troika" which needs to be considered when discussing the overall retention and enantioselectivity criteria. Essential are the multiple intramolecular interactions defined by the interaction sites of the SO and the SAs moieties together with their spatial arrangement. They represent the molecular recognition principles. The differences of the ΔG( R) and ΔG(S) values described by the ΔΔG values of the diastereomeric associates are directly related to the observed stereoselectivity values alpha (a) of the enantioselective (the "chiral") chromatographic system.

    In consideration of these basic principles we will then discuss the pros and cons of the most popular and promising so-called "chiral" columns operated in various MP modes. To date there is a wide selection of CSPs and "chiral" columns ranging from quite broadly applicable but also to very dedicated ones. It encompasses columns packed with ultra-high efficient CSPs enabling ultra-fast enantiomer separation but also CSPs and column formats useful for preparative enantiomer separation.
    Via the presentation of real world examples and applications we aim to give an overview of the state-of-the-art of this technology and to stress also specific challenges depending on the analytical questions.

    11.00-11.30 Coffee break
    11.30-13.00

    Short course 11

     

    Short course 11

    Advanced CE-MS approaches for metabolomics

    Speaker: Rawi Ramautar
    Leiden University, Leiden, Netherlands

    Biography

    In the field of metabolomics, capillary electrophoresis-mass spectrometry (CE-MS) has become a very useful analytical technique for the profiling of highly polar and charged metabolites in biological samples. In this course, a comprehensive overview of recent developments in CE-MS for metabolic profiling studies is provided. Topics that will be covered include CE separation modes, capillary coatings and recent interfacing designs for hyphenating CE to MS. In this context, special attention will be devoted to interfacing techniques allowing in-depth metabolic profiling studies by CE-MS. Throughout the discussion attention will be paid to practical aspects used for CE-MS-based metabolomics. The potential of this analytical technique for large-scale and quantitative clinical metabolomics studies is also addressed. Finally, the utility of CE-MS for metabolomics is demonstrated for selected clinical and biological problems, especially for volume-limited samples. Conclusions and perspectives on this unique analytical strategy are presented.

  • Hall U6 - 25 first floor
    08.30-09.30 Registration
    09.30-11.00

    Short course 4

     

    Short course 4

    Supercritical fluid chromatography (SFC) fundamentals

    Speaker: Caroline West
    University of Orleans, Orleans, France

    Biography

    This introductory course covers the basic elements of supercritical fluid chromatography (SFC). The basic theory of SFC and currently available commercial instrumentation is presented. The effects of operating parameters (choice of stationary phases and mobile phase composition, temperature, pressure) and specific aspects of detection (UV, ELSD, MS) are explained in the perspective of method development. In addition, varied applications are discussed (pharmaceuticals, cosmetics, food science, environment ...).

    Who Should Attend
    Everyone interested in using Supercritical Fluid Chromatography as an alternative for their current analysis, or those that are already using SFC, but want a better understanding of the underlying principles of separation performance, retention and selectivity.

    11.00-11.30 Coffee break
    11.30-13.00

    Short course 12

     

    Short course 12

    Fundamentals of SFC to guide practical method-development

    Speaker: Abhijit Tarafder
    Amgen Inc., USA

    Biography

    Modern Supercritical Fluid Chromatography (SFC) is mostly operated like HPLC. The only exception is that the former employs compressed CO2 as the principal solvent of the mobile-phase. The main physical difference between HPLC mobile-phases and SFC is the compressibility. SFC mobile-phases are significantly more compressible even when high composition of liquid organic solvent is added to CO2. E.g. isothermal compressibility of 80/20 (mass/mass,%) CO2/methanol mixture is 3.27 x 10-4 bar-1 at 20 ºC and 100 bar. In comparison compressibility of pure methanol is 1.08 x 10-4 bar-1, i.e. 3 times lesser under the same conditions (data from ref [1]). At higher temperatures, this difference is even starker.

    Method-development using a highly compressible mobile-phase can be confusing without a clearer insight on how compressibility vary with temperature and pressure and how such variations affect chromatographic behavior. Compressibility can also lead to unexpected consequences. E.g. in SFC any change in experimental methods and conditions that result into a change in system pressure, may affect chromatography. Change in particle size, column dimension or flow-rate, which do not affect analyte retention factor in HPLC, may do so in SFC. Another area of interest is in understanding the effect of pressure-drop on mobile-phase temperature variation. In ultra-performance LC, mobile-phase generally heats up caused by high pressure-drop. In SFC, although the pressure-drop is not significant, it can impart important temperature variations. Traditional belief is that compressible mobile-phase in SFC expands and cools under pressure-drop. In reality, mobile-phase in modern SFC can either cool, or heat-up like in LC, or may go through minimal temperature changes even with high pressure drop.

    It is not easy to describe in words how and under what conditions the effect of compressibility will affect chromatography; or when, because of a pressure-drop in mobile-phase, temperature will decrease or increase or not change. We need thermodynamic plots for better understanding of the situation. The objective of this course is to explain these topics with the help of two different thermodynamic plots - the isopycnic and the isenthalpic plots [2-3]. Isopycnic plots present constant density curves inside a pressure-temperature plane, whereas isenthalpic plots present constant enthalpy. First, construction of such plots will be described in details. Then, with the help of these plots, the convoluted nature of the physical properties of CO2-based mobile-phases, and their effect on chromatographic behavior in highly compressible regions will be explained.

    References

    • [1] E.W. Lemmon, M. Huber, M.O. McLinden, D.G. Friend, Standard Reference Data Program, Gaithersburg 9.0, National Institute of Standards and Technology, 2007.
    • [2] A. Tarafder, G. Guiochon, Journal of Chromatography A, 1218 (2011) 4576-4585.
    • [3] A. Tarafder, P. Iraneta, G. Guiochon, K. Kaczmarski, D. P. Poe, Journal of Chromatography A, 1366 (2014) 126-135
  • Hall U6 - 21 first floor
    08.30-09.30 Registration
    09.30-11.00

    Short course 5

     

    Short course 5

    New trends in LC-MS

    Speaker: Achille Cappiello
    University of Urbino, Urbino, Italy

    Biography

    This course is recommended to a wide audience of intermediate-level scientists, students and technicians in the field of analytical chemistry and related disciplines, such as biology, environment, clinical and food, among others. It will be also an extremely useful course for newcomers in the field willing to approach this subject for the first time. No previous knowledge of mass spectrometry is necessary, but an understanding of the fundamentals of liquid chromatography is beneficial. The focus has been addressed to the new trends, starting from the innovations of each technique (what is the vanguard in liquid chromatography; what is the new frontier in mass spectrometry) to the most progressive challenges of LC-MS. Researchers, practitioners, consultants working in any field in which LC-MS is the technique of choice will find this course valuable, with complete, up-to-date information on this fascinating and powerful technique. They will learn the state-of-the-art for the currently available LC-MS interfaces, their operational principles, as well as the 'new frontiers' and their application potential. A special focus has been given to 'high-end' topics such as high-resolution tandem mass spectrometry, single ion monitoring, multiple reaction monitoring, as well as to data processing to achieve the highest sensitivity and selectivity.

    11.00-11.30 Coffee break
    11.30-13.00

    Short course 13

     

    Short course 13

    Analytical characterization of protein biopharmaceuticals

    Speaker: Davy Guillarme
    University of Geneva, Geneva, Switzerland

    Biography

    The characterization of therapeutic proteins such as monoclonal antibodies (mAbs) represents a tremendous challenge. This short course will highlight the possibilities offered by all the different modes of chromatography (i.e. RPLC, SEC, IEX, HIC, HILIC) to rapidly and accurately characterize protein biopharmaceuticals (e.g., glycosylation, oxidation, deamidation, aggregation, glycation, lysine truncation...). The hyphenation of chromatographic approaches with mass spectrometry as well as the use of 2D-LC approaches will also be covered during the short course. Last but not least, the different levels of analysis of biopharmaceuticals will be discussed, including bottom-up analysis (2-5kDa), middle-up (25-100 kDa), and intact proteins analysis (150 kDa).

  • Hall U6 - 26 first floor
    08.30-09.30 Registration
    09.30-11.00

    Short course 6

     

    Short course 6

    3D-printing in separation science

    Speaker: Simone Dimartino
    The University of Edinburgh, Edinburgh, United Kingdom

    Biography

    This short course aims to offer an overview of how 3D printing is impacting the separation sciences in general, and chromatography in particular. 3D printing can seamlessly create bespoke models with complex shapes, using a range of materials, and with resolutions that can reach the submicron-scale. This capability opened a new way to fabricate stationary phases, column housings, filtration elements, extraction units and other devices of relevance to the separation science.

    This short course will open with a short introduction on 3D printing and the main printing techniques of relevance to us. Then, a selection of original research pieces will be presented, demonstrating how separation science is benefitting from 3D printers. Finally, current challenges as well as future opportunities will be discussed. This course should spur additional interest in the area and promote new ideas on how to employ 3D printers in our research.

    11.00-11.30 Coffee break
    11.30-13.00

    Short course 14

     

    Short course 14

    Rediscovering HPTLC and its hyphenations: with minimalism to maximal information on product quality and safety

    Speaker: Gertrud Morlock
    Justus Liebig University Giessen, Giessen, Germany

    Biography

    Strong features of high-performance thin-layer chromatography (HPTLC) are illustrated by examples. It is discussed how it can be exploited for the sake of your analytical task. An overview is given on the state-of-the-art HPTLC and its diverse couplings to mass spectrometry. Desorption- and elution-based couplings and their latest automations are discussed.
    Modern direct bioautography is focussed, as it is the most straightforward technique of effect-directed analysis. Latest novel workflows for biological and enzymatic assays led to sharply bounded active zones on a homogeneous background, and thus opened up new avenues. Reliable and meaningful autograms evaluated by densitometric measurements paved the way for quantitative direct bioautography. The trick of inverse scanning is needed to measure zones absorbing less than the background. Besides calibration via external standards, equivalency calculation with reference to a well-known active compound can be an alternative, if the active analyte is not identified or known. Just applied versus developed positive control levels are compared for equivalency calculation. The selection of a positive and negative control to be applied on each plate is explained. System blanks need to be performed for each sample set, too. Piezoelectric spraying, a new mode for covering the chromatogram with the assay, is compared with automated immersion.
    Latest trends in open source-based data evaluation and miniaturized planar chromato-graphy are illustrated that aim at an open source system which combines all steps of planar chromatograpy (all-in-one) in an online (fully automated) operation mode.

  • Hall U6 - 27 first floor
    08.30-09.30 Registration
    09.30-11.00

    Short course 7

     

    Short course 7

    Preparative chromatography

    Speaker: Attila Felinger
    University of Pécs, Pécs, Hungary

    Biography

    Large-scale separations have been intensively used in various fields such as pharmaceutical industry or biotechnology for the purification of target compounds. The aim of the short course is to provide separation scientists with a deeper understanding of the industrial scale separation process, with emphasis on the scale-up and optimization of large-scale process chromatography.
    The presentation will summarize the fundamentals and practice concerning the selection of the operational conditions of preparative chromatography by starting with an introduction to nonlinear chromatography. The consequences of column overloading, nonlinear isotherms and their general effects on the band profiles due to competition are discussed. The goals and target parameters of preparative separations, such as production rate, purity, yield, throughput, and purification costs, will be discussed.
    Various strategies to optimize preparative separations are overviewed. The advantages of the different objectives that consider the production rate, recovery yield or solvent consumption to achieve both productive and economical separations are discussed. Guidelines are given how to select the essential column design and experimental parameters to be optimized. The performance of isocratic, gradient elution, and displacement modes of chromatography as well as simulated moving bed separations are compared.
    The advantages of the modeling of the separation processes are demonstrated. Modeling will help save time and money when one tries to understand or optimize preparative separations.

    11.00-11.30 Coffee break
    11.30-13.00

    Short course 15

     

    Short course 15

    Preparative protein chromatography

    Speaker: Giorgio Carta
    University of Virginia, Charlottesville, Virginia, USA

    Biography

    This short course examines some of the fundamental underpinnings of protein chromatography and provides tools needed to understand and model preparative/process scale applications. The workshop will highlight differences between preparative protein chromatography and HPLC of small molecules. Mass transfer critically affects the former while packing quality is critically important for the latter. Column design and scale-up criteria are, thus, different in the two fields and will be emphasized in this presentation.

    • Protein adsorption isotherms
    • Protein mass transfer kinetics in chromatography particles
    • Characterization of protein chromatography columns according to the plate model
    • Gradient elution chromatography
    • Frontal loading and dynamic binding capacity
    • Scale-up of protein chromatography columns
  • Hall U6 - 28 first floor
    08.30-09.30 Registration
    09.30-11.00

    Short course 8

     

    Short course 8

    Two-dimensional liquid chromatography (2D-LC): a tutorial focusing on best practices

    Speaker: Dwight Stoll
    Gustavus Adolphus College College, St. Peter, Minnesota, USA

    Biography

    Speaker: Matthias Pursch
    Dow Deutschland Anlagen GmbH, Stade, Germany

    Biography

    Speaker: Stephan Buckenmaier
    Agilent Technologies, Waldbronn, Germany

    Biography

    Abstract: Two-dimensional liquid chromatography (2D-LC) can resolve difficult separation problems and has become important in industry for solving problems ranging from targeted analyses of structurally similar molecules to untargeted separations of highly complex samples. In 2D-LC, the effluent from a first dimension (1D) column is transferred to a second dimension (2D) column with the goal to resolve those analytes that co-eluted in 1D. In this tutorial we will discuss:

    • Concepts of targeted and comprehensive modes including a brief on the early history along with the theoretical underpinnings of the perceived advantages of 2D separations over their conventional 1D-LC counterparts.
    • Practical aspects of modern 2D-LC that are critical for the development of successful separations including experimental details, column selection, and the management of the interface between the two separation dimensions.
    • How to tackle analytical challenges that can make the difference between unimpressive and spectacular 2D-LC separations.
    • Recent applications of 2D-LC described in the literature that address problems in a variety of industries ranging from the analysis of small molecular weight compounds in polymers and biological materials to the analysis of food and (bio-) pharmaceuticals.
    11.00-11.30 Coffee break
    11.30-13.00

    Short course 16

     

    Short course 16

    Statistical analysis of chromatographic data: a practical guide

    Speaker: Gabriel Vivó Truyols
    Tecnometrix, Spain

    Biography

    These days, the enormous quantity of data generated by HPLC instruments demand a background knowledge on data analysis. This is especially necessary when high-data density detectors are used (e.g. high-resolution mass spectrometry). In order to gain most of the advantages of the powerful instrumentation, the data generated has to be properly explored and analysed. These days, the quality of the results depends critically on how the data analysis task has been performed. This course is directed to a wide audience of HPLC practitioners that are interested in improving their skills in data analysis. We will introduce the audience to basic concepts in HPLC optimization, as well as in pre-processing (e.g. alignment, peak detection), curve resolution and multivariate pattern recognition and modelling. Special emphasis will be given to the state-of-the-art techniques in data analysis for HPLC-MS, including peak resolution, screening and library search. We will discuss applications in a diversity of industries, including food, forensics, pharma, Oil & gas, and omics.

  • Milan Conservatorio
    "Giuseppe Verdi"
    08.30-09.30 Registration
    11.00-11.30 Coffee break
    16.00-19.00

    Milan Conservatorio "Giuseppe Verdi"

    16.00-19.00 Opening Ceremony

    16.00-16.30 Welcome - Chairs of HPLC 2019 Italy

    16.30-17.30 Award Presentation

    17.30-18.30 "Music for Leonardo" M. Kemp, Oxford University, UK
    and young musicians of the "Conservatorio della Musica Giuseppe Verdi di Milano"
    directed by maestri M. Baggio and S. Mormone

    18.30-19.00 "The art of chromatography" Attila Felinger, University of Pécs, Hungary

     

    Plenary

    Opening ceremony - Milan Conservatorio "Giuseppe Verdi"

    16.00 - 16.30 Welcome
    A. Cavazzini, Università degli Studi di Ferrara, Italy
    M. Morbidelli, Politecnico di Milano, Italy
    16.30 - 17.30 Award Presentation
    • Georges Guiochon Faculty Fellowship
    • Uwe D. Neue in Separation Science
    • Chromatographic Society Martin Medal
    • JFK Huber Lecture
    • Chromatographic Society Jubilee Medal
    17.30 - 18.30 Music for Leonardo
    M. Kemp, Oxford University, UK and young musicians of the "Conservatorio della Musica Giuseppe Verdi di Milano" directed by maestri M. Baggio and S. Mormone
    18.30 - 19.00 The art of chromatography
    A. Felinger, University of Pécs, Hungary
    19.00-21.00

    Welcome reception

     

     
Hall U6 - 06 ground floor Hall U6 - 07 ground floor Hall U6 - 08 ground floor Hall U6 - 09 ground floor Hall U6 - 01F ground floor Hall U6 - 01A ground floor
08.30-10.15 Registration
10.15-11.55

10.15-11.55

(MON 1.1)

Proteomics - 1

10.15-11.55

(MON 2.1)

Miniaturization & electrodriven technologies

10.15-11.55

(MON 3.1)

Fundamentals - 1, Column efficiency

10.15-11.55

(MON 4.1)

Food analysis - 1

   
12.20-14.00  

12.20-13.20

Vendor seminar
Merck

 

12.20-13.20

Vendor seminar
Agilent Technologies

12.20-13.20

Vendor seminar
Phenomenex

12.20-13.20

Vendor seminar
Waters Corporation

Lunch, Exhibition & Poster Session
14.00-15.35

14.00-15.35

(MON 1.2)

Sample preparation - 1

14.00-15.35

(MON 2.2)

Ion exchange chromatography & MS

14.00-15.35

(MON 3.2)

Fundamentals - 2, stationary phases

14.00-15.35

(MON 4.2)

Miniaturization & microfluidics

   
15.35-17.00 Coffee break, Exhibition & Poster Session
17.00-18.35

17.00-18.35

(MON 1.3)

Sample preparation - 2

17.00-18.35

(MON 2.3)

Biomedical applications

17.00-18.35

(MON 3.3)

Fundamentals - 3, stationary phases

17.00-18.35

(MON 4.3)

Innovative applications - 1

   
Hall U6 - 06 ground floor Hall U6 - 07 ground floor Aula Magna ground floor Hall U6 - 01E ground floor Hall U6 - 01F ground floor Hall U6 - 01A ground floor Hall U6 - 01B ground floor
08.30-10.10

08.30-10.10

(TUE 1.1)

LC-MS bioactive molecules - 1

08.30-10.10

(TUE 2.1)

Multidimensional chromatography - 1

08.30-10.10

(TUE 3.1)

Chiral separations - 1

08.30-10.10

(TUE 4.1)

Envinromental analysis

     
10.10-11.10 Coffee break, Exhibition & Poster Session
11.10-12.30

11.10-12.30

(TUE 1.2)

LC-MS bioactive molecules - 2

11.10-12.30

(TUE 2.2)

Multidimensional chromatography - 2

11.10-12.30

(TUE 3.2)

Chiral separations - 2

11.10-12.30

(TUE 4.2)

Pharmaceuticals and biopharmaceuticals - 1

     
12.30-14.00 Lunch, Exhibition & Poster Session
 

13.00-14.00

Vendor seminar
Shimadzu Europa GmbH

   

13.00-14.00

Vendor seminar
Molnár-Institute for Applied Chromatography

13.00-14.00

Vendor seminar
Thermo Fisher Scientific

13.00-14.00

Vendor seminar
Agilent Technologies

14.00-15.45

14.00-15.45

(TUE 1.3)

Biosample preparation

14.00-15.45

(TUE 2.3)

Omics - 1

14.00-15.45

(TUE 3.3)

Fundamentals - 4, retention & selectivity

14.00-15.45

(TUE 4.3)

Capillary electrophoresis - 1

     
15.45-16.35 Coffee break, Exhibition & Poster Session
16.35-18.00

16.35-18.00

(TUE 1.4)

Proteomics - 2

16.35-18.00

(TUE 2.4)

Glycomics & protein interactions

16.35-18.00

(TUE 3.4)

Data Analysis - 1

16.35-18.00

(TUE 4.4)

SFC-MS

     
18.00-19.00    

18.00-19.00

Separation Science Slam

       
19.00-19.30    

19.00-19.30

Separation Science Slam Apero

       
Hall U6 - 06 ground floor Hall U6 - 07 ground floor Aula Magna ground floor Hall U6 - 01E ground floor Hall U6 - 01F ground floor Hall U6 - 01A ground floor Hall U6 - 01B ground floor
08.30-10.20

08.30-10.20

(WED 1.1)

Forensic, doping and toxicology

08.30-10.20

(WED 2.1)

Pharmaceuticals and biopharmaceuticals - 2

08.30-10.20

(WED 3.1)

HILIC

08.30-10.20

(WED 4.1)

Instrumentation & quantitation

     
10.20-11.20 Coffee break, Exhibition & Poster Session
11.20-12.40

11.20-12.40

(WED 1.2)

Lipidomics

11.20-12.40

(WED 2.2)

3D Printing & Microchip

11.20-12.40

(WED 3.2)

SFC - 1

11.20-12.40

(WED 4.2)

Food analysis - 2

     
12.40-14.00 Lunch, Exhibition & Poster Session

13.00-14.00

Vendor seminar
Waters Corporation

     

13.00-14.00

Vendor seminar
Agilent Technologies

13.00-14.00

Vendor seminar
Thermo Fisher Scientific

13.00-14.00

Vendor seminar
Merck

14.00-15.45

14.00-15.45

(WED 1.3)

Omics - 2

14.00-15.45

(WED 2.3)

Pharmaceuticals and biopharmaceuticals - 3

14.00-15.45

(WED 3.3)

Fundamentals-5 Temperature responsive LC and selectivity

14.00-15.45

(WED 4.3)

Multidimensional chromatography - 3

     
15.45-16.35 Coffee break & Exhibition
16.35-18.00

16.35-18.00

(WED 1.4)

Omics - 3

16.35-18.00

(WED 2.4)

Miniaturization & LC-MS interface

16.35-18.00

(WED 3.4)

Quality control - 1

16.35-18.00

(WED 4.4)

Pharmaceuticals and biopharmaceuticals - 4

     
18.00-19.00    

18.00-19.00

HPLC Tube

       
19.00-19.30    

19.00-19.30

HPLC Tube Apero

       
20.30-24.00

Gala Dinner

Hall U6 - 06 ground floor Hall U6 - 07 ground floor Aula Magna ground floor Hall U6 - 01E ground floor
08.30-10.05

08.30-10.05

(THU 1.1)

Capillary electrophoresis - 2

08.30-10.05

(THU 2.1)

Preparative chromatography - 1

08.30-10.05

(THU 3.1)

HPTLC - 1

08.30-10.05

(THU 4.1)

SFC - 2 & data analysis - 2

10.05-11.10 Coffee break, Exhibition & Poster Session
11.10-12.55

11.10-12.55

(THU 1.2)

Quality control - 2

11.10-12.55

(THU 2.2)

Preparative chromatography - 2

11.10-12.55

(THU 3.2)

HPTLC - 2

11.10-12.55

(THU 4.2)

Innovative applications - 2

12.55-14.00 Lunch & Exhibition
14:00 - 16:15

Aula Magna

14.00 - 16.15 Closing Plenary Session

14.00 - 14.30 "Microscale Liquid Phase Separations Using Specific Interactions" K. Otsuka, Kyoto University, Japan

14.30 - 15.00 "Polymer brush bonded phases for MS-compatible mobile phases in protein separations" M. Wirth, Purdue University, USA

15.00 - 15.30 "Lipidomics: A window of opportunity for clinical analysis" Micheal Lämmerhofer, University of Tüebingen, Germany

15.30 - 15.45 Invitation from three future chairmen (HPLC2019 Kyoto, HPLC2020 San Diego, HPLC2021 Duesseldorf)

15.45 - 16.15 Closing ceremony (Csaba Horvath Young Scientist Award and Best Poster Award)

16.15-17.30 Farewell Drink
Poster Session

Applications - Anal. characterization of protein biopharmaceuticals

P 1Analytical challenges and strategies related to plant-derived vs mammalian cell-derived antibodies
S. Tengattini1, F. Rinaldi1, G. Massolini1, C. Temporini1, R. Venturi2, C. Pisano2, C. Camponeschi2
1Department of Drug Sciences, University of Pavia, Italy; 2Special Product’s Line S.p.A., Roma, Italy
P 2CVA-MS ANALYSIS TO INVESTIGATE THE EFFECTS OF LEACHABLES FROM SINGLE-USE BIOREACTORS ON MONOCLONAL ANTIBODIES
S. Carillo1, F. Füssl1, N.D. Garcia1, C. Ta1, P.S. Kelly2, N. Barron2, J. Bones2,3
1Characterisation and Comparability Laboratory, NIBRT. Foster Avenue, Mount Merrion, Blackrock, co. Dublin. Ireland; 2National Institute for Cellular Biotechnology, Dublin City University, Glasnevin, Dublin, Ireland; 3School of Chemical and Bioprocess Engineering, University College Dublin, Belfield, Dublin
P 3A NEW CHROMATOGRAPHIC APPROACH TO QUICKLY ASSESS ADCC ACTIVITY OF THERAPEUTIC ANTIBODIES
P. Endres, L. Salim, R. Römling, E. Müller
Tosoh Bioscience GmbH, Griesheim, Germany
P 4ASYMMETRICAL FLOW FIELD-FLOW FRACTIONATION AND SIZE-EXCLUSION CHROMATOGRAPHY FOR STUDYING THE AGGREGATION OF BETA-D-GALACTOSIDASE
I.K. Ventouri1,2, A. Astefanei1, E. Kaal3, G.W. Somsen4, P. Schoenmakers1
1University of Amsterdam, van't Hoff Institute for Molecular Sciences-Analytical Chemistry Group, Amsterdam, The Netherlands; 2TI-COAST, Amsterdam, The Netherlands; 3DSM Biotechnology Center, DSM, Delft, The Netherlands; 4Vrije Universiteit Amsterdam, Division of BioAnalytical Chemistry, Amsterdam, The Netherlands
P 5AN AUTOMATED WORKFLOW FOR ON-COLUMN DIGESTION AND ANALYSIS OF MONOCLONAL ANTIBODIES
M. Nordgren, A. Nägeli, S. Björk, H. Nyhlén, J. Sjögren, F. Olsson
Genovis AB, Lund, Sweden
P 6CHARACTERIZATION OF MONOCLONAL ANTIBODY THERAPEUTICS BY CAPILLARY ELECTROPHORESIS ELECTROSPRAY IONIZATION MASS SPECTROMETRY
K. Müller, L. Stock, H. Stutz
Department of Biosciences, University of Salzburg, Austria; Christian Doppler Laboratory for Innovative Tools for Biosimilar Characterization, Salzburg, Austria
P 7METHOD DEVELOPMENT AND PURIFICATION STRATEGIES OF RAW THERAPEUTIC PEPTIDE MIXTURES BY MEANS OF PREPARATIVE CHROMATOGRAPHY
G. Lievore1, C. De Luca1, S. Felletti1, M. Macis2, A. Ricci2, W. Cabri2, O.H. Ismail1, M. Catani1, M. Morbidelli3, A. Cavazzini1
1Department of Chemical and Pharmaceutical Sciences, University of Ferrara, Italy; 2R&D, Fresenius Kabi iPSUM, Villadose, Rovigo, Italy; 3Dept. of Chemistry and Applied Biosciences, Institute for Chemical and Bioengineering ETH, Zurich, Switzerland
P 8Multi-attribute Analysis of Monoclonal Antibodies by 3D-LC and Q-TOF Mass Spectrometry
G. Vanhoenacker1, I. Vandenheede1, P. Sandra1, K. Sandra1, S. Krieger2, S. Schneider2, U. Huber2
1Research Institute for Chromatography (RIC), Kortrijk, Belgium; 2Agilent Technologies, Waldbronn, Germany
P 9Good peak shape and recovery for phosphorylated compounds – Analysis of nucleotides employing an LC with a fully inert flow path
F. Rieck2, K. Sandra1, J. Vandenbussche1, G. Vanhoenacker1, R. t’Kindt1, P. Sandra1, S. Krieger2, S. Schneider2, U. Huber2
1Research Institute for Chromatography (RIC), Kortrijk, Belgium; 2Agilent Technologies, Waldbronn, Germany
P 10Alternative mobile phases in cation-exchange chromatography applied for separation of monoclonal antibodies
E. Farsang1, A. Murisier2, K. Horváth1, A. Beck3, R. Kormány4, D. Guillarme2, S. Fekete2
1Department of Analytical Chemistry, University of Pannonia, Veszprém, Hungary; 2School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Geneva, Switzerland; 3Center of Immunology Pierre Fabre, Saint-Julien-en-Genevois, France; 4Egis Pharmaceuticals Plc., Budapest, Hungary
P 11Development of new spongy-monolithic columns for selective separation of extracellular vesicles
S. KATO1, T. NAITO1, T. KUBO1, K. AKIYOSHI2, K. OTSUKA1
1Graduate School of Engineering, Department of Material Chemistry, Kyoto University, Japan; 2Graduate School of Engineering, Department of Polymer Chemistry, Kyoto University, Japan
P 12High-resolution, high-throughput characterization of protein therapeutic size variants by size exclusion chromatography
V. Qin1, A. Coffey2, J. Meixner3*
1Agilent Technologies, Wilmington, USA; 2Agilent Technologies, Church Stretton, UK; 3Agilent Technologies, Waldbronn, Germany
P 13Site-specific conjugation of metal-chelating polymers for radioimmunotherapy applications
S. Lin1, J. Baek1, B.J. Agnew2, P.J. Le3, V. Facca4, E. Lu3, M.A. Winnik3, R.M. Reilly4
1Chromatography Columns and Consumables, Thermo Fisher Scientific, Sunnyvale, CA; 2Licensing and Commercial Supply, Thermo Fisher Scientific, Eugene, USA; 3Departments of Chemistry, University of Toronto, Canada; 4Pharmaceutical Sciences, University of Toronto, Canada
P 14Development of novel reversed-phase packing material for improved separation of protein biopharmaceuticals including intact antibodies
D. Esser, K. Tsutsui, C. Matsumura, N. Shoji, H. Kanezaki, N. Kuriyama
YMC Europe GmbH, Dinslaken, Germany; YMC Co., Ltd., Kyoto, Japan
P 15Comparison of Imaged Capillary Isoelectric Focusing and Weak Cation Exchange Chromatography for Analysis of Charge Variants of Therapeutic Monoclonal Antibodies
S. Tremintin, J. Baek, S. Lin
Thermo Fisher Scientific, Sunnyvale, USA
P 16A universal mobile phase system for simple pH optimization of mobile phases used for ion exchange chromatography and hydrophobic interaction chromatography
S. Tremintin, J.M.T. Wong, J. Baek, S. Lin
Thermo Fisher Scientific, Waltham, USA
P 17Comparative analysis of fluorophore labeled N-glycans using liquid phase separation methods
C. Filep1, B. Borza1, M. Szigeti1, Z. Demianova2, A. Guttman1,2
1Horváth Csaba Laboratory of Bioseparation Sciences, Research Center for Molecular Medicine, Faculty of Medicine, University of Debrecen, Hungary; 2Sciex, Brea, USA
P 18Measurement of aggregation in high concentration biopharmaceutical formulations by microbore SEC with UV imaging detection
D. Goodall*, P. Jepson
Paraytec Ltd., York, UK
P 19ORTHOGONAL METHODS IN STABILITY TESTING OF LACTASE ENZYME, A MODEL OF BIOLOGICALS
B. Dalmadi Kiss1, M. Király1, L. Drahos2, P. Horváth3, A. Mirzahosseini3, G. Pálfy4, L. Márk5, I. Klebovich1, I. Antal1, K. Ludányi1
1Department of Pharmaceutics, Faculty of Pharmacy, Semmelweis University, Budapest, Hungary; 2MS Proteomics Research Group, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Budapest, Hungary; 3Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Semmelweis University, Budapest, Hungary; 4Laboratory of Structural Chemistry and Biology, Eötvös Loránd University, Budapest, Hungary; 5Department of Biochemistry and Medical Chemistry, University of Pécs, Medical School, Hungary
P 20Creation, implementation and transferability of a Glucose Unit (GU) library for HILIC-FLD analysis of APTS-labelled released N-glycans
P. Widdowson1, A. Eckard2, J. Young2, S. Carillo3, J. Bones3, K. Salomon2
1Thermo Fisher Scientific, Runcorn, UK; 2Thermo Fisher Scientific, Oyster Point, USA; 3National Institute for Bioprocessing Research and Training (NIBRT), Dublin, Ireland
P 21Stability-indicating analytical approach for lactoferrin
R. Roškar, N. Osel, T. Planinšek Parfant, J. Trontelj, A. Kristl
University of Ljubljana, Faculty of Pharmacy, Ljubljana, Slovenia

Applications - Biomedical and clinical separations/Pharmaceutical analysis

P 22FACTORIALLY DESIGNED MIXED MICELLAR LIQUID CHROMATOGRAPHIC METHOD FOR SIMULTANEOUS DETERMINATION OF NORFLOXACIN AND TINIDAZOLE
S.F. El-Malla, A. Hassan Kamal
Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Tanta University, Egypt
P 23DEVELOPMENT OF STABILITY-INDICATING HPLC METHOD FOR QUANTIFICATION OF VANCOMYCIN HYDROCHLORIDE IN INJECTABLE PHARMACEUTICAL DOSAGE FORM
A. Serri, A. Zarghi, H. Moghimi, A. Mahboubi
Shahid beheshti university of medical sciences
P 24APPLICATION OF LC-MS/MS TO IN VITRO STUDY OF RELATIONSHIP BETWEEN SELECTED POLYPHENOLS AND TWO HUMAN CELL LINES: FETAL OSTEOBLASTIC HFOB 1.19 AND OSTEOSARCOMA SAOS-2
J. Gasik1,2, A. Strzelecka-Kiliszek2, M. Biesaga1, S. Pikuła2
1Faculty of Chemistry, University of Warsaw, Poland; 2Department of Biochemistry, Nencki Institute of Experimental Biology, Warsaw, Poland
P 25EMPLOYING MODERN LIQUID CHROMATOGRAPHY TECHNOLOGY TO SCALE A USP GRADIENT METHOD ON A SINGLE LIQUID CHROMATOGRAPHIC SYSTEM
A. Dlugasch, J. Simeone, P. McConville
Waters Corporation, Milford, Massachusetts, USA
P 26APPLICATION OF LIQUID CHROMATOGRAPHY/MASS SPECTROMETRY FOR ELECTROCHEMICAL OXIDATION OF ANTIBIOTICS IN COMPARISON TO IN VITRO AND IN VIVO METHODS
M. Szultka-Młyńska1, K. Pauter1, W. Kupczyk2, B. Buszewski1
1Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Poland;
2Department of Surgery, Collegium Medicum, Nicolaus Copernicus University, Poland
P 27BARCODE ANALYSIS BY LC/MS WITH MULTIPLE AMPLIFICATION STRATEGIES FOR CANCER BIOMARKER DETECTION
N.M. Fang, C.Y. Lee, Y.Z. Hsieh
Department of Applied Chemistry, National Chiao Tung University, Hsinchu, Taiwan, R.O.C.
P 28ANALYSIS OF VASOPRESSIN ANALOGUES IN WATER SOLUTION USING A REVERSED-PHASE TRAP COLUMN AND A HILIC ANALYTICAL COLUMN
M. Malm
Global Pharmaceutical Development, Ferring Pharmaceuticals A/S, Copenhagen, Denmark
P 29DETECTION OF ILLEGALLY ADDED PHARMACEUTICALS IN THE MEDICATIONS FROM REGISTERED CHINESE MEDICINE PRACTITIONERS
H.K. Lam
Government Laboratory, Hong Kong SAR, China
P 30HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY IN NON-CLINICAL TOXICOLOGY RESEARCH OF CONTROLLED RELEASE PHARMACEUTICAL DOSAGE FORMS IN MICROPARTICLES OF A BIODEGRADABLE ORGANIC POLYMER
S. Zhao, D. Roman, S. Benmakrelouf, Y. Danilova, D. Guérette
Charles River Laboratories, Montreal ULC (CR-MTL), Senneville, Canada
P 31UHPLC-UV COUPLED TO A SINGLE QUADRUPOLE MASS DETECTOR FOR CONFIRMATION AND QUANTITATION OF A GENOTOXIC IMPURITY IN A DRUG SAMPLE
G. Greco, S. Fabel, S. Grosse, M. De Pra, F. Steiner
Thermo Fisher Scientifc, Germering, Germany
P 32HPLC-DAD-MS STABILITY-INDICATING METHOD FOR PROPOSED FIXED-DOSE COMBINATION OF AZATHIOPRINE AND FOLIC ACID
E. Brusač, M.L. Jeličić, I. Džajić, D. Amidžić Klarić, B. Nigović, A. Mornar
Department of Pharmaceutical Analysis, Faculty of Pharmacy and Biochemistry, University of Zagreb, Croatia
P 33NOVEL RP-HPLC-DAD METHOD FOR SIMULTANEOUS DETERMINATION OF AMINOSALYCILATES AND FOLIC ACID ASSAY IN PROPOSED FIXED DOSE COMBINATIONS FOR TREATMENT OF INFLAMMATORY BOWEL DISEASE
M.L. Jeličić1, E. Brusač1, D. Amidžić Klarić1, B. Nigović1, N. Turk2, A. Mornar1
1Department of Pharmaceutical Analysis, Faculty of Pharmacy and Biochemistry, University of Zagreb, Croatia; 2Clinical Hospital Center Zagreb, Croatia
P 34AN LC-MS/MS METHOD FOR DETERMINATION OF TRIPLE DRUGS COMBINATION OF VALSARTAN, AMLODIPINE AND HYDROCHLOROTHIAZIDE IN HUMAN PLASMA FOR BIOEQUIVALENCE STUDY.
B. Arafat
Faculty of Pharmacy & Medical Sciences, Al-Ahliyya Amman University, Jordan center for pharmaceutical research, Amman, Jordan
P 35Screening of Western Drug Adulterants in Complementary Health Products by Liquid Chromatography/Mass Spectrometry/Diode Array Detector
Y.L. Quek1, W.S. Sim2, K. Chiang3, C.Y. Gan3, X. Ge1, N.P. Cheah1, M.Y. Low1
1Pharmaceutical Division, Applied Sciences Group, Health Sciences Authority, Singapore; 2Department of Chemistry, Faculty of Science, National University of Singapore, Singapore; 3Chemistry and Biological Chemistry Division, School of Physical and Mathematical Sciences, Nanyang Technological University, Singapore
P 36Predicting skin permeability of pharmaceutical and cosmetic compounds using various liquid chromatographic methods
Y. Grooten, D. Mangelings, Y. Vander Heyden
Department of Analytical Chemistry, Applied Chemometrics and Molecular Modelling, Vrije Universiteit Brussel (VUB), Belgium
P 37Charged Aerosol Detection: Factors Affecting Uniform Analyte Response
C. Paul, K. Lovejoy, F. Steiner, P. Gamache
Thermo Fisher Scientific, Germering, Germany
P 38Simultaneous determination of ribonucleoside and deoxyribonucleoside triphosphates in biological samples by hydrophilic interaction liquid
chromatography coupled with tandem mass spectrometry

Z. Kong1, S. Jia1, A.L. Chabes1, P. Appelblad2,3, R. Lundmark4,5, T. Moritz6, A. Chabes1,5
1Department of Medical Biochemistry and Biophysics, Umea University, Sweden; 2Department of Pharmacology and Clinical Neuroscience, Umea University, Sweden; 3Merck Chemicals and Life Science AB, Solna, Sweden; 4Department of Integrative Medical Biology, Umea University, Sweden; 5Laboratory for Molecular Infection Medicine Sweden (MIMS), Umea University, Sweden; 6Umea Plant Science Centre (UPSC), Department of Forest Genetics and Plant Physiology, Umea, Sweden
P 39New cell separation method using the thermoresponsive surface modified with cell adhesion peptides
M. Shimura, K. Hanaya, Y. Hiruta, K. Nagase, H. Kanazawa
Graduate School of Pharmaceutical Sciences, Keio University, Tokyo, Japan
P 40Development of a two-dimensional liquid chromatographic method for analysis of urea cycle amino acids
Y. Sumida1, K. Hamase2, T. Funatsu1, M. Tsunoda1
1Faculty of Pharmaceutical Sciences, The University of Tokyo, Japan; 2Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan
P 41Analysis of compounds with distinct polarities in biological matrices: a case example of JAS-2
H. Bavlovič Piskáčková1, A. Reguli1, H. Jansová2, P. Brázdová3, M. Štěrba3, P. Štěrbová-Kovaříková1
1Department of Pharmaceutical Chemistry and Pharmaceutical Analysis, Faculty of Pharmacy in Hradec Králové, Charles University, Hradec Králové, Czech Republic; 2Department of Biochemical Sciences, Faculty of Pharmacy in Hradec Králové, Charles University, Hradec Králové, Czech Republic; 3Department of Pharmacology, Faculty of Medicine in Hradec Králové, Charles University, Hradec Králové, Czech Republic
P 42Advancing the peak production rate in reversed-phase gradient LC at 1500 bar to profile antibiotics samples that differ in complexity
E. Uushona1, T. Themelis1, M. De Pra2, F. Steiner2, K. Broeckhoven1, J. De Vos1, S. Eeltink1
1Vrije Universiteit Brussel, Department of Chemical Engineering, Brussels, Belgium; 2Thermo Fisher Scientific, Germering Germany
P 43A Validated HPLC-MS Method Based on Porous Graphitic Carbon Column for Determination of Twelve Thiopurine Nucleotides
D. Pecher1,2, S. Dokupilová1,2, Z. Zelinková3, J. Lučeničová4, P. Mikuš1,2
1Department of Pharmaceutical Analysis and Nuclear Pharmacy, Faculty of Pharmacy, Comenius University in Bratislava, Slovak Republic; 2Toxicological and Antidoping Center, Faculty of Pharmacy, Comenius University in Bratislava, Slovak Republic; 3Department of Gastroenterology, St Michael’s Hospital, Bratislava, Slovak Republic; 4Department of Biochemistry & Hematology, St Michael’s Hospital, Bratislava, Slovak Republic
P 44Biochromatography, a promising technique in early drug discovery
F. Tsopelas1, P. Danias1, E. Notari1, D. Anagnostopoulou1, A. Pappa1, A. Tsantili-Kakoulidou2
1Laboratory of Inorganic and Analytical Chemistry, School of Chemical Engineering, National Technical University of Athens, Greece; 2Department of Pharmaceutical Chemistry, School of Pharmacy, National and Kapodistrian University of Athens, Greece
P 45Screening therapeutics according to their uptake across the blood-brain barrier: A high throughput method based on immobilized artificial membrane liquid chromatography-diode-array-detection coupled to electrospray-time-of-flight mass spectrometry
G. Russo1, L. Grumetto2, R. Szucs3, F. Barbato2, F. Lynen4
1Separation Science Group, Department of Organic and Macromolecular Chemistry, Ghent University, Belgium; 2Department of Pharmacy, School of Medicine and Surgery, University of Naples Federico II, Italy; 3Pfizer Global R&D, Kent, United Kingdom; 4Separation Science Group, Department of Organic and Macromolecular Chemistry, Ghent University, Belgium
P 46Paradigm shift towards Method Lifecycle Management - A practical approach in establishing a SFC-based purity method for carbamazepine
A. Schmidt, M. Stanic
Chromicent GmbH, Berlin, Germany
P 47Azithromycin analysis according to latest US Pharmacopoeia monographs using HPLC-ECD
D. Friscione1, L. van Heerwaarden2, J.P. Chervet2, M. Eysberg2
1Alfatech SpA, Genova, Italy; 2Antec Scientific, Zoeterwoude, The Netherlands
P 48Determination of immune system activation markers, uric acid and creatinine in human saliva using HPLC
A. Vernerová1,2, L. Kujovská Krčmová1,2
1Department of Analytical Chemistry, Faculty of Pharmacy in Hradec Králové, Charles University, Czech Republic; 2Department of Clinical Biochemistry and Diagnostics, University Hospital Hradec Králové, Czech Republic
P 49Development and validation of two orthogonal HPLC methods for the study of the accelerated stability assessment program (ASAP) of Acetylsalicylic acid
P. Appelblad1,3, A. Speltini2, G. Marrubini2
1Department of Pharmacology, Umea University, Sweden; 2Department of Drug Sciences, University of Pavia, Italy; 3Merck Chemicals and Life Science AB, Solna, Sweden
P 50Ultra-high performance liquid chromatography in bioactivation study of an anticancer drug sobuzoxane
P. Reimerová1, A. Jirkovská2, H. Bavlovič Piskáčková1, P. Štěrbová-Kovaříková1
1Department of Pharmaceutical Chemistry and Pharmaceutical Analysis, Faculty of Pharmacy in Hradec Králové, Charles University, Akademika Heyrovského 1203, Czech Republic; 2Department of Biochemical Sciences, Faculty of Pharmacy in Hradec Králové, Charles University, Akademika Heyrovského 1203, Czech Republic
P 51UHPLC-MS/MS determination of urinary retinol as a potential new biomarker in clinical
K. Kučerová1,2, L. Kujovská Krčmová1,2, L. Matysová1
1Department of Analytical Chemistry, Faculty of Pharmacy in Hradec Králové, Charles University, Hradec Králové, Czech Republic; 2Department of Clinical Biochemistry and Diagnostics, University Hospital Hradec Králové, Czech Republic
P 52Simple and rapid measurement of encapsulation efficiency of drug in liposomes using a novel monolithic silica column with nanoparticle exclusion chromatography
K. Miyashita1, E. Yamamoto2,3, S. Miyazaki1, C. Aoyama1, I. Kushida2, T. Hasebe2, M. Kato4

1GL Sciences Inc.; 2Formulation Research Laboratories, Pharmaceutical Science and Technology Core Function Unit, Medicine Development Center, Eisai Co., Ltd.; 3National Institute of Health Sciences; 4School of Pharmacy, Showa University
P 53Rapid high-performance liquid chromatographic determination of colloidal silver–induced oxidative stress biomarker in microbially inoculated media
K.C. Hsu1, Y.C. Chen2, J.R. Hsieh1, P.F. Hsu1, Y.Y. Chao3, Y.L. Huang1,2
1Department of Medical Laboratory Science and Biotechnology, College of Health Sciences, Kaohsiung Medical University, Kaohsiung, Taiwan, R.O.C; 2Department of Laboratory Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan, R.O.C; 3Department of Public Health, College of Health Sciences, Kaohsiung Medical University, Kaohsiung, Taiwan, R.O.C
P 54Optimisation of oligonucleotide separations on ion-exchange chromatography
D. Esser, A. Matsui, S. Nozawa, N. Kuroda
YMC Europe GmbH, Dinslaken, Germany; YMC Co., Ltd., Kyoto, Japan
P 55Development of Methods for the Separation of Major Cannabinoids and Terpenes using six Column Selectivity Screening
A. McKeown1, G. Lo1, I. Phillips2, P. Bridge2
1Advanced Chromatography Technologies Ltd., Aberdeen, Scotland, UK; 2Avantor, VWR International Ltd., Lutterworth, UK
P 56Pharmaceutical Applications of Ion Chromatography-Mass Spectrometry (IC-MS)
B. Jackson1, A. Ray1, T. Corry2, Z. Lewis2
1Pharmaceutical Technology and Development, AstraZeneca, UK; 2Department of Chemistry, University of Manchester, UK
P 57Maximisation of peak capacity for peptide mapping using a column coupling approach with solid-core columns
A.P. McKeown1, P. Bridge2, I. Phillips2
1Advanced Chromatography Technologies Ltd., Aberdeen, Scotland, UK; 2Avantor, VWR International Ltd., Lutterworth, UK
P 58LC/MS/MS Analysis of Fentanyl and Related Analogs Using Biocompatible Solid Phase Microextraction
1P. Lewits; 2E. Barrey; 3C.E. Muraco
1Merck KGaA, Chromatography & Instrumental Analysis, Darmstadt, Germany; 2Supelco Inc, Sample Preparation and Workflow R&D, Bellefonte, USA; 3Supelco Inc, Liquid Separations R&D, Bellefonte, USA
P 59Identification and quantification of lipids in vaccines by HPTLC
Cotte Jean-François 1, Martial Fabien 1, Dubayle Jean 1, Talaga Philippe 2
1 Analytical Sciences, Sanofi Pasteur, Marcy l'Etoile, France
2 Global MTech, Sanofi Pasteur, Marcy l'Etoile, France
P 60Development of UHPLC method for determination of salicylic acid, methyl-salicylate and ethylsalicylate in pharmaceutical formulations
L. Matysová1, K. Kučerová1,2, N. Kosáčková1
1Department of Analytical Chemistry, Faculty of Pharmacy in Hradec Králové, Charles University, Czech Republic; 2Department of Clinical Biochemistry and Diagnostics, University Hospital Hradec Králové, Czech Republic
P 61Development and validation of two orthogonal HPLC methods for the study of the accelerated stability assessment program (ASAP) of Acetylsalicylic acid
P. Appelblad1,3, A. Speltini2, G. Marrubini2
1Department of Pharmacology, Umea University, Sweden; 2Department of Drug Sciences, University of Pavia, Italy; 3Merck Chemicals and Life Science AB, Solna, Sweden
P 62Chemiluminescent nitrogen detection – a neglected LC detection technique for determination of relative response factors in pharmaceutical analysis
J. Neumann, A.H. Schmidt, M. Stanic
Chromicent GmbH, Berlin, Germany
P 63Application of dried blood spots for quantitative analysis of biological metabolites
H.D. Cho, Y.Y. Kim, J.Y. Lee, Y. Lee, E. Jo, S. Seo, G. Kim, S.B. Han*
Department of Pharmaceutical Analysis, College of Pharmacy, Chung-Ang University, Seoul, Republic of Korea
P 64Studying organoid drug metabolism using liquid chromatography (UHPLC and nano-format) and mass spectrometry
A. Mrsa1, I.C.S. Boger1, E. Nerem2, F.S. Skottvoll1, S. Harrison3, G. Sullivan3, E. Lundanes1, I.L. Bogen2, S.R. Wilson1
1Detpartment of Chemistry, University of Oslo, Norway; 2Section for Drug Abuse Research, Department of Forensic Sciences, Division of Laboratory Medicine, Oslo University Hospital, Norway; 3Hybrid Technology Hub-Centre of Excellence, Institute for Basic Medical Sciences, Faculty of Medicine, University of Oslo, Norway
P 65Protein analysis of liver organoids with minaturized LC-MS: nano and PLOT format
A. Hermansen1,2, M. Schüller1,2, F.S. Skottvoll1,2, T. Hara3, G. Desmet4, E. Lundanes1, S.R. Wilson1,2
1Department of Chemistry, University of Oslo, Norway; 2Hybrid Technology Hub Centre of Excellence, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, Norway; 3Division of Metabolomics, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan; 4Department of Chemical Engineering, Vrije Universiteit Brussel, Belgium
P 66Validated HPLC methods with fluorescence detection for the quantitation of ciprofloxacin and ampicillin antibiotics in mouse faecal samples to study bacterial multidrug resistancy
M.K. Nyírő1, J. Erdőssy1, B. Varga1, F. Balázs Farkas3, D. Szabó3, V. Horváth1,2
1Budapest University of Technology and Economics, Department of Inorganic and Analytical Chemistry, Budapest, Hungary; 2MTA-BME Computation Driven Chemistry Research Group, Budapest, Hungary; 3Semmelweis University, Institute of Medical Microbiology, Budapest, Hungary
P 67A Novel Solution for Vitamin K1 and K2 Analysis in Human Plasma by LC-MS/MS
T. Kahler, S.H. Liang, R. Alagandula, F. Carroll, P. Connolly, D. Bell
Restek Corporation, Bellefonte, USA
P 68Analysis of Phosphatidylethanol (PEth) in Human Whole Blood by LC-MS/MS
T. Kahler, S.H. Liang, R. Alagandula, F. Carroll, P. Connolly
Restek Corporation
P 69A lab-on-a-disk device for isolation and identification of parasite eggs in stool
A. Kryj1, S. Sukas1, B. Van Dorst2, O. Lagatie2, W. De Malsche1, L. Stuyver2
1Vrije Universiteit Brussel, Belgium; 2Janssen Diagnostics, Beerse, Belgium
P 70a bio-analytical case study of monoamine HPLC-ECD analysis in rat brain matrix
S. Andresen, B. Anzelius, A. Josefsson, A.S. Hansen, D. Kirik, U. Schagerlöf
Lund University, Experimental Medical Science, Lund, Sweden
P 71The investigation of hydroxy acid - lactone interconversion of statins in biological matrix. Application of hplc-ms/ms methods for clinical trials
D.A. Biianova, S.I. Kononova, A.A. Nikitina, A.V. Grigoriev, A.A. Sidorova
Bioanalytical Laboratory CSU “Analytical Spectrometry”, Saint-Petersburg, Russia
P 72Determination of ibandronic acid in human plasma by LC-MS/MS for pharmacokinetic applications
D. Iaroshenko, A. Nikitina, A. Grigoriev, A. Sidorova
CSU Analytical Spectrometry, Saint-Petersburg, Russian Federation

Applications - Development of chromatographic methods for QC

P 73METHOD DEVELOPMENT AND VALIDATION: WHERE IS ANALYTICAL CHEMIST REALLY NEEDED?
A. Laaniste, K. Herodes
University of Tartu, Institute of Chemistry, Tartu, Estonia
P 74COMPARISON OF THE PROPOSALS FOR THE ESTIMATION OF PREDICTION AND CONTENT TOLERANCE INTERVALS IN CHROMATOGRAPHIC ASSAY METHODS VALIDATION
J.M. Roussel, M. Righezza
Consultant, Macon, France; Aix-Marseille universite, Marseille, France
P 75SELECTION OF THE MOST APPROPRIATE STATIONARY PHASE FOR CHROMATOGRAPHIC DETERMINATION OF COMPONENTS OF SIMILAR POLARITY IN METHOD DEVELOPMENT
S. Zubčić1, V.G. Marković2, R. Truban-Žulj1
1Agency for Medicinal products and Medical Devices of Croatia, Zagreb, Croatia; 2University of Zagreb, Faculty of Chemical Engineering and Technology, Zagreb, Croatia
P 76NEW TRENDS ON ANALYTICAL METHOD VALIDATION: THE ANALYTICAL PROCEDURE LIFECYCLE
KIMBER BARNETT; GREG MARTIN; HORACIO PAPPA

K. Barnett, G. Martin, H. Pappa
United States Pharmacopeia, Rockville, USA
P 77ROBUSTNESS ASSESSMENT IN COMPUTER-ASSISTED LIQUID CHROMATOGRAPHY PROCEDURES BASED ON DESIRABILITY FUNCTIONS
S. Triñanes, P. Rodriguez-Mier, C. Cobas, E. Sánchez, R. Phan-Tan-Luu, R. Cela
Dpt. of Analytical Chemistry, Research Institute of Food Analysis, University of Santiago de Compostela, Spain; Centre for Information Technologies, University of Santiago de Compostela, Spain; Mestrelab Research S.L., Spain; Mestrelab Research S.L., Spain; University Paul Cezanne, Marseille, France; Dpt. of Analytical Chemistry, Research Institute of Food Analysis, University of Santiago de Compostela, Spain
P 78A SAFE AND PRECISE METHOD TO ANALYZE 4-TERT-BUTYLCATECHOL IN PRESSURIZED LIQUEFIED HYDROCARBON STREAMS USING LIQUID CHROMATOGRAPHY
A. König, M. Dunkle
Dow Benelux, Analytical Science, Terneuzen, The Netherlands
P 79QUALITY BY DESIGN IN THE DEVELOPMENT OF HYDROPHILIC INTERACTION LIQUID CHROMATOGRAPHY METHOD WITH GRADIENT ELUTION FOR THE ANALYSIS OF OLANZAPINE
A. Tumpa1, J. Maksić Janković2, G. Forsdahl3, B. Jančić-Stojanović1
1Department of Drug Analysis, Faculty of Pharmacy, University of Belgrade, Serbia; 2Department of Drug Control and Examination, Service for Pharmaceutical Activity, Military Medical Academy, Belgrade, Serbia; 3Department of Pharmacy, University of Tromsø, Norway
P 80STREAMLINED METHOD DEVELOPMENT FOR ACTIVE PHARMACEUTICAL INGREDIENTS IN COLD AND FLU MEDICATION USING A SYSTEMATIC PROTOCOL
M. Maziarz, P. Rainville
Waters Corporation, Milford, USA
P 81ANALYSIS OF ATROPISOMERS OF API’S BY HPLC AND SFC
A. Hessler1, E. Zafrir Ilan1, T. Ho1, M. Stanic2, A. Schmidt2
1Gilead Sciences Inc., Foster City (CA); 2Chromicent GmbH, Berlin (DE)
P 82ANALYTICAL STRATEGIES TO QUANTITATE VOLATILE DEGRADANTS IN DRUG SUBSTANCE
H. Marshall, Y. Gao
Gilead Sciences Inc., Foster City (CA)
P 83DOE APPROACH TO EFFICIENT ASSAY AND DEGRADATION PRODUCT METHOD DEVELOPMENT FOR FIXED DOSE COMBINATION TABLETS
T. Waki, C.I. Alvarado, N. Gumataotao, C. Chan, V. Rucker
Gilead Sciences Inc., Foster City (CA)
P 84CHARGED AEROSOL DETECTION AND METHOD TRANSFER OF COMPENDIAL, INCLUDING USP, METHODS
M. Pietsch, K. Lovejoy, I. Acworth, P. Gamache
Thermo Fisher Scientific, Dreieich, Germany; Thermo Fisher Scientific, Germering, Germany; Thermo Fisher Scientific, Chelmsford, USA; Thermo Fisher Scientific, Chelmsford, USA
P 85Modernization of USP Monographs for Naphazoline Hydrochloride and Pheniramine Maleate Ophthalmic and Nasal Solutions
M. Maziarz, S. Ramakrishna, P. Rainville, S. Naughton
Waters Corporation, Milford, USA; U.S. Pharmacopeial Convention, Rockville, USA
P 86Online SEC-MR-NMR – A Powerful Tool in Polymer Analysis, with a Direct Correlation between Molar Mass Distribution (MMD) and Chemical Composition (CC)
C. Botha, J. Höpfner, M. Pollard, M. Wilhelm
Karlsruhe Institute of Technology, Chemistry and Biosciences, Institute for Technical Chemistry and Polymer Chemistry, Karlsruhe, Germany
P 87Multivariable modeling of ternary linear gradient parameters for method optimization based on precise prediction of retention behavior
T. Kawabe, M. Ono, M. Ohara
Analytical & Quality Evaluation Research Laboratories, Daiichi Sankyo Co., Tokyo, Japan
P 88System Performance Check for using Gas Chromatography with Flame Ionization Detection (GC-FID) in Analysis of C8-C40 Saturated Hydrocarbon Compounds
J.J. Seo1, H.Y. Kim1, G.S. Hwang2
1Korea Basic Science Institute, Scientific Instrument Reliability Assessment Center, Daejeon, Republic of Korea; 2Korea Basic Science Institute, Western Seoul Center, Seoul, Republic of Korea
P 89Online SEC-IR(QCL-EC) – Coupling of SEC with mid-IR Quantum Cascade Lasers for Specific Functional Group Detection in Analysis of Polymers and Copolymers
M. Pollard, C. Botha, J. Kübel, M. Wilhelm
Institute for Chemical Technology and Polymer Chemistry, Polymeric Materials Research Group, K.I.T., Karlsruhe, Germany
P 90Application of the Lifecycle Management to the analytical chromatographic methods used for the Quality Control of Itraconazole drug products
R. Klinkenberg, A. Brouwers, C. Köttgen, B. Mertens, J. Harzimont, P. Stiernon, B. Streel
Galephar M/F, Marche en Famenne, Belgium
P 91Optimization and Validation of Online Column-Switching assisted HPLC-Spectrometric Method for Quantification of Dansylated Endocannabinoids and Neurotransmitters in Animal Models of Brain Diseases
M. Baranyi, B. Sperlágh
Department of Pharmacology, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, Hungary
P 92DoE as an effective and comprehensive tool for RPLC method robustness evaluation
L. Volta e Sousa, A.F. Ferreira
Hovione Farmaciência S.A., R&D Analytical Development, Estrada do Paço do Lumiar, Campus do Lumiar, Lisboa, Portugal
P 93Chromatographic Support for Artemether Formulation Development: Hypercarb-facilitated, sample solvent directed assay and related substances assay
W.J. Lough, N. Khan, N. Mohd, O. Bamgbala, X. Lin, J. Ong, I. Ghulam, D. Small
School of Pharmacy and Pharmaceutical Sciences, University of Sunderland, United Kingdom

Applications - Electrically-driven separations

P 94ELECTROMEMBRANE EXTRACTION BASED ON AGAROSE GEL: AN EFFICIENT METHOD FOR EXTRACTION AND DETERMINATION OF PHENOLIC ACIDS
S. Asadi, S. Nojavan, M. Behpour
Department of analytical chemistry and pollutants, Shahid Beheshti University, Tehran, Iran
P 95DEVELOPMENT OF GLASS-BASED MICROCHIPS FOR ELECTROCHROMATOGRAPHIC SEPARATIONS
A. Kryj1, M. Baca1, E. Naghdi1,2, P. Gelin1, S. Sukas1, W. De Malsche1
1µFlow group, Department of Bioengineering Sciences, Vrije Universiteit Brussel, Belgium; 2Department of Chemistry, Shahid Beheshti University, Teheran, Iran
P 96EVALUATION OF ANTI-MIRNA OLIGONUCLEOTIDES USING CAPILLARY ELECTROPHORESIS WITH LASER-INDUCED FLUORESCENCE (CE-LIF)
E. Ban1,2, D.K. Chae1, A. Kim2, E.J. Song1
1Molecular Recognition Research Center, Korea Institute of Science and Technology, Seoul, South Korea; 2College of Pharmacy, CHA University, Seongnam, South Korea
P 97Affinity Capillary Electrophoresis of Human Serum Albumin and Small Molecules
A Comparison between Mobility Shift and Frontal Analysis

M.O. Stein1, H. Nevídalová2, R. Ratih1, Z. Glatz2, H. Wätzig1
1Institute of Medicinal and Pharmaceutical Chemistry, TU Braunschweig, Germany; 2Department of Biochemistry, Faculty of Science, Masaryk University, Brno, Czech Republic
P 98Effects of the tunable EOF on the resolution and analysis time in CE separation of helquats
V. Šolínová, D. Koval
Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences, Czech Republic
P 99Field-amplified sample injection and sweeping micellar electrokinetic chromatography in analysis of glyphosate and aminomethylphosphonic acid in wheat
R. Gotti1, J. Fiori2, S. Bosi3, G. Dinelli3
1Department of Pharmacy and Biotechnology, University of Bologna, Italy; 2Department of Chemistry “G. Ciamician”, University of Bologna, Italy; 3Department of Agricultural and Food Sciences, University of Bologna, Italy
P 100Separation of perfluorinated surfactants by non-aqueous capillary electrophoresis coupled with contactless conductivity detector
W. Grochocki, M. Breadmore
Australian Centre for Research on Separation Science, School of Natural Science, University of Tasmania, Hobart, Australia
P 101Comparison of electrodriven focusing methods for improvement of two-dimensional separations
J. Váňová, J. Sadílková, P. Česla
Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Czech Republic
P 102Separation of histones from cancer cell extracts by capillary electrophoresis with UV absorbance detection
Y.J. Lai, Z.X. Lu, P.L. Chang
Department of Chemistry, Tunghai University, Taichung, Taiwan
P 103Simplify modification mapping at the intact protein level with on-line separation and analysis by CE-MS
S. Lock1, W. Belloni2
1Sciex, Warrington, UK; 2Sciex, Milan, Italy

Applications - Environmental analysis

P 104HILIC – EFFECT OF SALT-CONTAINING AQUEOUS MATRICES
M.J.C. Brekelmans, C.W.S. Goossens, E.M. Reingruber, K. Scase
Charles River laboratories,'s-Hertogenbosch, the Netherlands
P 105IDENTIFICATION OF BISPHENOL A IN BOTTLED WATER USING SOLID-PHASE EXTRACTION AND ULTRA-PERFORMANCE LIQUID CHROMATOGRAPHY–TANDEM MASS SPECTROMETRY
A.M. Alammari1, M. Rizwan Khan1, A. Aqel1, Z.A. Alothman1, I.H. Alsohaimi2, A. Alsubhi1
1Department of Chemistry, College of Science, King Saud University, Riyadh, Saudi Arabia; 2Chemistry Department, College of Science, Jouf University, Sakaka, Saudi Arabia
P 106A newly developed procedure for quantifying leaf pigments of broadleaf trees
F. Petibon, M.W.I. Schmidt, G.L.B. Wiesenberg
Soil science and biogeochemistry, Department of Geography, University of Zurich, Switzerland
P 107ANALYTICAL PLATFORM FOR NON-TARGETED FINGERPRINTING ANALYSIS OF METAL(LOID)-CONTAINING ORGANIC COMPOUNDS IN ENVIRONMENTAL SAMPLES
P. Denti, H.R. Hansen, L.M.V. Malmquist, D.B.A. Malheiro, G. Tomasi, J.H. Christensen
1,3,5,6Department of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg; 2Danish Environmental Protection Agency, Odense, Denmark; 4MS-Omics, Vedbaek, Denmark
P 108POLYCYCLIC AROMATIC HYDROCARBONS (PAHS) IN THE RED MULLET (MULLUS BARBATUS) USING QUECHERS EXTRACTION AND UHPLC-FLUORESCENCE DETECTION
E. Frapiccini1, M. Panfili1, S. Guicciardi1, A. Santojanni1, M. Mauro1, M. Betti1, C. Truzzi2, M. Antonucci2, F. Girolametti2, S. Illuminati2, G. Scarponi2, A. Annibaldi2
1National Research Council, CNR-IRBIM, Ancona, Italy; 2Department of Life and Environmental Sciences, Università Politecnica delle Marche, Ancona, Italy
P 109RAPID DETERMINATION OF THE GLYPHOSATE IN SOIL BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
K. Ashimuly, Z. Tukenova, U. Alimov, M. Alimzhanova
Department Natural Science Disciplines, Kazakhstan Engineering and Technological University, Almaty, Kazakhstan; Department of Chemistry and Chemical Technology, Al-Farabi Kazakh National University, Almaty, Kazakhstan
P 110Optimization of SPE extraction and HPLC separation for monitoring of pharmaceuticals in water
P. Šilarová1, N. Roulová2, L. Česlová3, D. Šilha4
1Department of Inorganic Technology, University of Pardubice, Czechia; 2Department of Biological and Biochemical Science, University of Pardubice, Czechia; 3Department of Analytical Chemistry, University of Pardubice, Czechia; 4Department of Biological and Biochemical Science, University of Pardubice, Czechia
P 111Instability and Complexity of Polycyclic Musk Galaxolide
D.M. Makey1, A. Crawford1, D.C. Harmes1, K.H. Wammer2, D.R. Stoll1
1Gustavus Adolphus College, Saint Peter, Minnesota, USA; 2University of Saint Thomas, Saint Paul, Minnesota, USA
P 112Trace analysis of haloacetic acids in environmental water samples using high-pressure ion chromatography-mass spectrometry
J. Boertjes, E. Gilchrist
School of Chemistry, University College Cork, Ireland; a School of Chemistry, University College Cork, Ireland, b Environmental Research Institute, University College Cork, Ireland
P 113DETERMINATION OF POLYCYCLIC AROMATIC HYDROCARBONS FROM SOIL BY USING HPLC
I. Kiss
Department of Analytical and Environmental Chemistry, University of Pécs, Hungary
P 114Determination of haloacetic acids formed in model systems by liquid chromatography-tandem mass spectrometry
A. Bednáriková1, K. Munka2, J. Ilavský3, D. Barloková3, S. Varga2, M. Slovinská2, A. Vajíčeková2, K. Jančurová2
1Water Research Institute, National Water Reference Laboratory, Bratislava, Slovakia; 2Water Research Institute, Division of Water Quality, Bratislava, Slovakia; 3Slovak University of Technology in Bratislava - Faculty of Civil Engineering, Department of Sanitary and Environmental Engineering, Bratislava, Slovakia
P 115Development of a multiclass method for pharmaceuticals determination in water applying rotating-disk sorptive microextraction coupled offline to UHPLC-MS/MS
W. Calzadilla1, C.G. Peña1, R. Salazar2, M. Aranda1
1University of Concepcion, Bio Bio Region. Chile; 2University of Santiago, Metropolitan Region. Chile
P 116Molecular-level Investigation of soils contaminated oil from Gulf War after 30 Years using FT-ICR MS and GCxGC/HRMS
M. Park1, E. Cho2, M. Hur3, G. Kang4, S. Kim2, Y.H. Kim1
1Biomedical Omics Group, Korea Basic Science Institute, Cheongju, Republic of Korea; 2Dept. of Chemistry, Kyungpook National University, Daegu, Republic of Korea; 3Dept. of Botany and Plant Sciences, and Institute of Integrative Genome Biology, University of California, Riverside, USA; 4Dept. of Environmental Sciences, Hankuk University of Foreign Studies, Yongin, Republic of Korea
P 117Emerging contaminant occurrence in a London urban river system measured using passive sampling and liquid chromatography-high resolution accurate mass spectrometry
A.K. Richardson, A. Gavell, G.R. Fones, G.A. Mills, A.M. Edge, D.A. Cowan, D.J. Neep, L.P. Barron
School of Population Health & Environmental Sciences, King's College London, United Kingdom; Natural Resources Wales, Llanelli, United Kingdom; School of Pharmacy and Biomedical Sciences, University of Portsmouth, United Kingdom; Agilent Technologies LDA UK Ltd., Church Stretton, United Kingdom
P 118Development of an innovative method to determine glyphosate, AMPA and glufosinate in fresh water using on-line SPE combined with HPAEC-MS
R. Zangrando1, E. Barbaro1, M. Feltracco2, C. Barbante1,2, Andrea Gambaro1,2
1Institute for the Dynamics of Environmental Processes CNR, Mestre, Italy; 2Department of Environmental Sciences, Informatics and Statistics, Ca’ Foscari University of Venice, Italy
P 119Simultaneous determination of phthalate metabolites in DBS (dried blood spot) using liquid chromatography with tandem mass spectrometry (LC-MS/MS)
J.Y. Lee, H.D. Cho, E. Jo, Y. Lee, Y.Y. Kim, S. Sumin, K. Gunwoo, S.B. Han
Department of Pharmaceutical Analysis, College of Pharmacy, Chung-Ang University, Seoul, Republic of Korea
P 120Origin of life: Experimental evolution of functional vesicles in hydrothermal environments
Y. Danisan1,2, M. Sulkowski1,2, U. Schreiber3, C. Mayer4, O.J. Schmitz1,2
1Institute of Applied Analytical Chemistry (AAC), University of Duisburg-Essen, Germany; 2Teaching and Research Centre for Separation (TRC), Essen, Germany; 3Department of Geology, University of Duisburg-Essen, Germany; 4Institute of Physical Chemistry, CENIDE, University of Duisburg-Essen, Germany
P 121Simultaneous determination of phthalate metabolites in dried blood spot using liquid chromatography with tandem mass spectrometry
J.Y. Lee, H.D. Cho, E. Jo, Y. Lee, Y.Y. Kim, S. Sumin, K. Gunwoo, S.B. Han
Department of Pharmaceutical Analysis, College of Pharmacy, Chung-Ang University, Seoul, Republic of Korea
P 122Impact of large historical eruptions on fluoride records obtained from Antarctic ice cores using capillary ion chromatography
E. Sanz Rodriguez1, C. Plummer2, M. Nation3, A. Moy2,3, M. Curran2,3, P.R. Haddad1, B. Paull1
1Australian Centre for Research on Separation Science, School of Natural Sciences, University of Tasmania, Australia; 2Antarctic Climate and Ecosystems Cooperative Research Centre, University of Tasmania, Australia; 3Australian Antarctic Division, Tasmania, Australia
P 123Analysis of Bisphenols and Perfluorinated Compounds by LC-MS/MS Using Raptor 1.8 µm Columns
P. Connolly, L.A. Wiest, S.H. Liang, J. Konschnik, T. Kahler
Restek Corporation, Bellefonte, USA
P 124Direct analysis of illicit and pharmaceutical substances in wastewater using rapid LC-MS/MS
K.T. Ng1, H. Rapp-Wright1, L. Couchman2, Leon Barron1
1Dept. Analytical, Environmental & Forensic Sciences, King's College London, UK; 2Toxicology Unit, Department of Clinical Biochemistry, King's College Hospital NHD Foundation Trust, London, UK
P 125Ion Chromatography Determination of Nitrite, Nitrate, and Bromide in Seawater by UV/Vis Detection after Suppression
P. Jochems, V. Kraft, G. Schad, R. Ludwig
Shimadzu Europa GmbH, Duisburg, Germany
P 126Simultaneous enrichment and hplc-ms/ms quantification of several classes of algal toxins in environmental waters
F. Merlo1, D. Piparo1, F. Maraschi1, A. Speltini2, A. Profumo1
1Department of Chemistry, University of Pavia, Italy; 2Department of Drug Sciences, University of Pavia, Italy
P 127SPECIATION ANALYSIS OF ORGANOTIN COMPOUNDS IN ENVIRONMENTAL SAMPLES
G. Vergine, E. Sarti, V. Costa, A. Cavazzini, L. Pasti
Università degli Studi di Ferrara, Italy
P 128Determination of blood plasma testosterone and corticosterone in barn swallow (Hirundo rustica)
Z. Bílková1, M. Kotasová Adámková1,2, O. Kauzál3, Z. Šimek1, T. Albrecht3
1Research Centre for Toxic Compounds in the Environment, Masaryk University, Brno, Czech Republic; 2Department of Botany and Zoology, Masaryk University, Brno, Czech Republic; 3Institute of Vertebrate Biology, Academy of Sciences, Studenec, Czech Republic; 4Department of Zoology, Charles University, Prague, Czech Republic

Applications - Food analysis

P 129A SINGLE-STEP SOLID-PHASE EXTRACTION FOR DETERMINATION OF PATULIN IN APPLE JUICE BY LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY
X. Li, H. Li
National Institute of Metrology
P 130ESTABLISHMENT OF A RAPID ANALYTICAL METHOD FOR SIMUTANEOUS DETERMINATION OF THE EU PRIORITY POLYCYCLIC AROMATIC HYDROCARBONS (PAHS) IN CHAR-GRILLED CHICKEN DRUMSTICKS
D.J. Yang1,2, C.F. Chiang2,3, K.C. Hsu2,4, C.Y. Cho2, T.Y. Tsai2, C.H. Hsu1,2
1Institute of Food Safety and Health Risk Assessment, National Yang-Ming University, Taipei, Taiwan; 2Master Program of Food and Drug Safety, China Medical University, Taichung, Taiwan; 3Department of Health Risk Management, China Medical University, Taichung, Taiwan; 4Department of Nutrition, China Medical University, Taichung, Taiwan
P 131DETERMINATION OF POLYMER ADDITIVES IN FOODS AND DRINKS PACKED WITH PLASTIC BY MAGNETIC SOLID-PHASE EXTRACTION OF AMINO GROUP MODIFIED MAGNETIC MESOPOROUSE SILICA MICROSPHERES COUPLED WITH HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
X. Liu, Z. Guo, X. Zhao, Q. Li, Y. Wei
State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, China
P 132LACTOSE CONTENT IN ‘LACTOSE-FREE’ LABELLED DAIRY PRODUCTS (LACTOSE INTOLERANCE)
J.P. Chervet1, H.J. Brouwer1, M. Eysberg1, D. Friscione2
1Antec Scientific, Zoeterwoude, The Netherlands; 2Alfatech SpA, Genova, Italy
P 133EFFECTS OF HARVEST TIME ON PHENOLIC COMPOSITIONS AND ANTIOXIDANT ATTRIBUTES OF LEAVES OF EIGHT PERSIMMON VARIETIES
P.J. Wu1,2, Y.L. Chang3,4, D.J. Yang1, J.T. Li5, H.L. Li4
1Institute of Food Safety and Health Risk Assessment, National Yang-Ming University, Taipei, Taiwan; 2Dikma Technologies Inc., Markham, Canada; 3Miaoli District Agricultural Research and Extension Station, Council of Agricultural, Miaoli, Taiwan; 4Department of Horticulture, National Chung Hsing University, Taiwan; 5Department of Medical Applied Chemistry, Chung Shan Medical University, Taichung, Taiwan
P 134MOLECULAR IMPRINTED POLYMER@MAGNETIC NANOPARTICLES COMBINED WITH LIQUID CHROMATOGRAPHY MASS SPECTROMETRY FOR DETERMINATION OF GLYCOALKALOIDS IN POTATO
C.H. Yeh, C.Y. Chen, M.R. Lee
Department of Chemistry, National Chung Hsing University, Taichung, Taiwan
P 135CHEMICAL PROFILE OF MORUS NIGRA EXTRACT OBTAINED BY PRESSURIZED HOT WATER EXTRACTION
N. Nastić1, A. Segura-Carretero2,3, J. Švarc-Gajić1, I.l Borrás-Linares3, J. Lozano-Sánchez3
1Faculty of Technology, University of Novi Sad, Novi Sad, Serbia; 2Department of Analytical Chemistry, Faculty of Science, Granada, Spain; 3Functional Food Research and Development Centre (CIDAF), Health Science Technological Park, Granada, Spain
P 136A RAPID ULTRASOUND-ASSISTED DISPERSIVE LIQUID-LIQUID MICROEXTRACTION FOLLOWED BY LIQUID CHROMATOGRAPHY COUPLED TO A MASS SPECTROMETRY FOR DETERMINATION OF ARTEMISININ IN TILAPIA MUSCLE
M.P. Soares1, L.S. Shiroma2, I.L. Cardoso1, F.G. Sampaio3, C.M. Jonsson3, F.T. Rantin1, S.C.N. Queiroz3
1Department of Physiological Sciences, Federal University of São Carlos, Brazil; 2Institute of Chemistry, UNICAMP, Department of Analytical Chemistry, Campinas, Brazil; 3Embrapa Environment, Brazilian Agricultural Research Corporation (EMBRAPA), Jaguariúna, Brazil
P 137A NEW GASTRO-INTESTINAL SYSTEM TO EVALUATE THE EFFECT OF FOOD METHYLGLYOXAL
L. Ferron1,2, R. Colombo1, M. Bordiga3, C. Garino3, M. Paolillo1, A. Papetti1,4
1University of Pavia, Department of Drug Sciences, Pavia Italy; 2FlaNat Research Italia Srl, Rho (MI) Italy; 3University of Eastern Piedmont, Department of Pharmaceutical Sciences, Novara, Italy; 4euFAN Srl, University of Pavia, Italy
P 138HPLC-UV FINGERPRINTING TO ADDRESS NUT AUTHENTICATION BY CHEMOMETRIC METHODS
G. Campmajó1, R. Saez-Vigo1, G.J. Navarro1, N. Núñez1, J. Saurina1,2, L. Puignou1,2, O. Núñez1,2,3
1Department of Chemical Engineering and Analytical Chemistry, University of Barcelona, Spain; 2Research Institute in Food Nutrition and Food Safety, University of Barcelona, Santa Coloma de Gramanet, Barcelona, Spain;
3Serra Húnter Program, Generalitat de Catalunya, Barcelona, Spain
P 139AN EMPIRICAL APPROACH TO PREDICT SEPARATION OF WATER-SOLUBLE VITAMINS USING REVERSED-PHASE CHROMATOGRAPHY
S. Marten, S. Grosse, S.H. Park, M. De Pra, F. Steiner
Thermo Fisher Scientific, Germering, Germany
P 140ANALYSIS OF CHERRY ALLERGENS BY LIQUID CHROMATOGRAPHY/TANDEM MASS SPECTROMETRY USING STABLE ISOTOPE-LABELLED PEPTIDES
K. Ippoushi, Y. Tanaka, M. Wakagi, N. Hashimoto, Y. Takano-Ishikawa
Food Research Institute, National Agriculture and Food Research Organization, Tsukuba, Japan
P 141AUTOMATED METHOD DEVELOPMENT IN HPLC FOR THE QUANTITATIVE DETERMINATION OF CATECHINS IN TEA
S. Marten, S. Grosse, S.H. Park, M. De Pra, F. Steiner
Thermo Fisher Scientific, Germering, Germany
P 142ANALYSIS OF ACTIVE CANNABIS COMPOUNDS IN EDIBLE FOOD PRODUCTS WITH A HPLC-UV METHOD
M. Ye, K.K. Stenerson, O.I. Shimelis
MilliporeSigma, Bellefonte, USA
P 143QSight LC-MS/MS as a screening tool to detect traces of milk and egg allergens in incurred food
L. Monaci1, E. De Angelis1, R. Pilolli1, A. Ganci2
1Institute of Sciences of Food Production - Italian National Research Council (ISPA-CNR), Bari, Italy; 2Perkin Elmer Italia S.p.A., Milan, Italy
P 144Quantification of pesticide residues in fatty food matrixes: strategy of sample preparation HPLC-MS/MS analysis
S. BELARBI1,2, M. VIVIER2, P. CARDINAEL1, V. AGASSE1
1Normandie Univ, Laboratoire SMS-EA3233, UNIROUEN, Mont-Saint-Aignan Cedex, France; 2SGS France laboratoire de Rouen, Technopôle du Madrillet, Saint Etienne du Rouvray, France
P 145Toolbox for studying the chemistry of light induced degradation (TOOCOLD): towards an online degradation system
M. den Uijl, B. Pirok, P. Schoenmakers, M. van Bommel
Van 't Hoff Institute for Molecular Sciences, University of Amsterdam, The Netherlands
P 146Determination of cholecalciferol in milk and dairy product samples by using dispersive liquid-liquid microextraction method combined with liquid chromatography
T. Hájek, V. Hladíková
Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Czech Republic
P 147Authentication of Garcinia cambogia supplements by HPLC-UV-MS
A. Mena1, A.J. Belleizac2, D.M. Chito2, M.P. Rada-Mendoza2, A.I. Ruiz-Matute1, A.C. Soria1, M.L. Sanz1
1Instituto de Química Orgánica General (IQOG-CSIC), Madrid, Spain; 2Departamento de Química, Facultad de Ciencias Naturales,Exactas y de la Educación, Universidad del Cauca, Popayán, Colombia
P 148Profiling of phenolic flavourings in whiskey by reversed phase liquid chromatography with electrochemical detection at a boron doped diamond electrode
P.E. Hayes1,2, A. Buzid1,2, J.H. T. Luong1,2, E.S. Gilchrist1,2, J.D. Glennon1,2
1Innovative Chromatography Group, Irish Separation Science Cluster (ISSC), University College Cork, Ireland; 2School of Chemistry and the Analytical, Biological and Chemistry Research Facility (ABCRF), University College Cork, Ireland
P 149Native amino acid analysis in wine by HILIC separation and detection with single quadrupole mass spectrometry
S.H. Park, H. Herrmann, K. Lovejoy, S. Grosse, M. De Pra, S. Meding, M. Menz, F. Steiner
ThermoFisher Scientific, Chromatography and Mass Spectrometry Division, Dreieich, Germany; Thermo Fisher Scientific, Chromatography and Mass Spectrometry Division, Germering, Germany
P 150The Advantages of TLC as a Quick Screening and Crosscheck Method for Natural Products using the Quantification of α- and β- Acids in Hop as an Example
J. Engemann1, V. Pilakowski1, M. Oberle1, M. Burholt1, M. Schulz1, M. Bäumle2*




1Merck KGaA, Life Science, Darmstadt, Germany; 2Sigma-Aldrich Chemie GmbH, Switzerland (Sigma-Aldrich Chemie GmbH is a subsidiary of Merck)
P 151Sulfur speciation analysis in wine by HPLC-ICPMS/MS
B. Lajin, W. Goessler
Institute of Chemistry – Analytical Chemistry for Health and Environment (ACHE), University of Graz, Austria
P 152Chlorogenic acids and caffeine in green and roasted coffee by HPLC-DAD
A. Schmidt, L. Macheiner, S. van den Oever, H.K. Mayer
Department of Food Science and Technology, Food Chemistry Laboratory, BOKU - University of Natural Resources and Life Sciences Vienna, Austria
P 153Improved Fermentation Process Monitoring using Dual Injection UHPLC System
T. Yamaguchi, K. Koterasawa, H. Terada,K. Matsumoto
Shimadzu Corporation Analytical & Measuring Department, Kyoto, Japan
P 154Determination of Naturally occurring benzoic acid and sorbic acid in vegetable, animal and their processed foods
S.H. Kim, S. Lee, J.S. So, M.Y. Lee, J.H. Lee, H.S. Lim, Y.S. Kang
Food additives and Packaging Division, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety, Cheongju, Republic of Korea
P 155Determination of Chloramphenicol by QuEChERS and HPLC-MS/MS combination in Twenty Two Different Matrices
T. Śniegocki, B. Sell, M. Giergiel, A. Posyniak
Department of Pharmacology and Toxicology, National Veterinary Research Institute, Pulawy, Poland
P 156Non-targeted screening and identification of unknown compound in food supplement using LC/QTOF
S. Kotucova, L. Lakosilova, R. Stepan, M. Kubik, P. Cuhra
Czech Agriculture and Food Inspection Authority (CAFIA), Prague, CZECH REPUBLIC
P 157Oligosaccharides determination in Trentino honey by High-Performance Anionic-Exchange Liquid Chromatography coupled with Mass Spectrometry (HPAEC-MS)
R. Tedesco1,3, E. Barbaro2, G. Capodaglio1, A. Rizzoli3, P. Fontana3, V. Malagnini3, A. Gambaro1
1Department of Environmental Sciences, Informatics and Statistics, University of Venice, Ca' Foscari, Venice-Mestre, Italy; 2Institute for the Dynamics of Environmental Processes, CNR, Venice-Mestre, Italy; 3Fondazione Edmund Mach (FEM)-Centro Trasferimento Tecnologico, Trento, Italy
P 158Simple U-HPLC-MS method for evaluation of sweeteners, preservatives and purine alkaloids in soft drinks
A. Krmela, V. Schulzova, J. Pulkrabova, J. Hajslova
University of Chemistry and Technology, Department of Food Analysis and Nutrition, Prague, Czech Republic
P 159Comparison of commercial and natural black and red currant juices by LCxLC-DAD-qTOF-MS coupling mix-mode separation with reversed phase mode
L. Montero1,2, S.W. Meckelmann1,2, K. Hellmann1,2, O.J. Schmitz1,2
1University of Duisburg-Essen, Applied Analytical Chemistry, Essen, Germany; 2Teaching and Research Center for Separation (TRC), University of Duisburg-Essen, Germany
P 160Effective clean-up of 7 regulated mycotoxins in grain and subsequent determination by HPLC-MS/MS
T. Gersthagen, H.R. Wollseifen, T. Kretschmer
MACHERY-NAGEL GmbH & Co. KG, R&D Chromatography, Dueren, Germany
P 161Fast and simple method for the determination of tranquillizers in kidney by liquid chromatography with tandem mass spectrometry
B. Sell, T. Śniegocki, M. Giergiel, A. Posyniak
Department of Pharmacology and Toxicology, National Veterinary Research Institute, Pulawy, Poland
P 162Determination of cannabidiol and additional cannabinoid content in hemp tea
G. Schad, V. Kraft, P. Jochems, R. Ludwig, U. Oppermann
Shimadzu Europa GmbH, Analytical Business Unit, Duisburg, Germany
P 163A Perfect Pairing: HILIC and Superficially Porous Particles in Food Analyses
P. Connolly, L. Wiest, D. Li, J. Konschnik, T. Kahler
Restek Corporation, Bellefonte, USA
P 164Optimizing a 190+ Pesticides Multi-Residue Screening Workflow for the Preparation and Analysis of Produce by LC-MS/MS
P. Connolly, L.A. Wiest, D. Li, A.M. Pavkovich, J. Konschnik, T. Kahler, D. Bell
Restek Corporation, Bellefonte, USA
P 165Quantitation of Mycotoxins in Four Food Matrices Comparing Stable Isotope Dilution Assay (SIDA) with Matrix Matched Calibration Methods by LC-MS/MS
T. Kahler, D. Li, J. Steimling, J. Konschnik, P. Connolly
Restek Corporation
P 166Functional evaluation of fresh (FG) and black (BG) Chiloe’s giant garlic (Allium ampeloprasum L.) by High-Performance Thin-Layer Chromatography-Bioassay-Mass Spectrometry
D. Peterssen-Fonseca, J. Carrasco, K. Henríquez-Aedo, M. Aranda
Laboratory of Advanced Research on Foods and Drugs, Department of Food Science and Technology, Faculty of Pharmacy, University of Concepcion, Barrio Universitario s/n, Concepcion, Chile

Applications - Forensic, doping and toxicology

P 167HOW SAFE IS YOUR GARDEN?
S. Bowerbank, J. Dean, M. Carlin
Department of Applied Sciences, Northumbria University, Newcastle upon Tyne, UK
P 168ANALYSIS OF MICROCYSTINS LR, YR, AND RR IN BIOLOGICAL FLUIDS BY 2D-LC TECHNOLOGY
B. Garcia-Barboza1, C.R. Mallet2, S.R. Botch-Jones1
1Boston University School of Medicine, Boston, USA; 2Waters Corporation, Milford, USA
P 169SINGLE-INJECTION SCREENING OF 664 FORENSIC TOXICOLOGY COMPOUNDS USING AN INNOVATIVE BENCHTOP HIGH RESOLUTION MASS SPECTROMETER
B. Nieland3, O.G. Cabrices1, A. Wang1, X. He1, H. McCall1, L. Baker1, A. Taylor2
1SCIEX, Redwood City, USA; 2SCIEX, Concord, Ontario, Canada; 3SCIEX, Nieuwerkerk a/d IJssel, Netherlands
P 170COMPARISON OF THE KINETICS OF DYES DEGRADATION OF HANDWRITTEN STROKES SUBJECTED TO DIFFERENT TYPES OF ARTIFICIAL AGING AND STUDIED USING CHROMATOGRAPHY MASS-SPECTROMETRY AND STATISTICAL DATA PROCESSING
D. Baygildieva, A. Sherstneva, I. Plyushchenko, I. Ananieva, O. Shpigun, I. Rodin
Chemistry Department, Lomonosov Moscow State University, Moscow, Russia
P 171Gas chromatographic fingerprint analysis for the comparison of seized cannnabis samples
A. Slosse1,2, F. Van Durme2, N. Samyn2, D. Mangelings1, Y. Vander Heyden1
1Department of Analytical Chemistry, Applied Chemometrics and Molecular Modelling, Vrije Universiteit Brussel (VUB), Belgium; 2Department Drugs and Toxicology, National Institute for Criminalistics and Criminology (NICC), Brussels, Belgium
P 172Development of a rapid LC-MS/MS methodology for the analysis of Novel Psychoactive Substances (NPS) - regioisomeric phenidine derivatives
J.K. Field1, C. Titman2, C. Hinz2, M.R. Euerby1,2, O.B. Sutcliffe3
1Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, UK; 2Shimadzu UK Limited, Mill Court, UK; 3Manchester DRug Analysis and Knowledge Exchange (MANDRAKE), School of Science and the Environment, Faculty of Science and Engineering, Manchester Metropolitan University, John Dalton Building, UK
P 173Determination of methadone, citalopram and clomipramine and its metabolites in post mortem skeletal tissue
M. Vandenbosch1*, L. Rooseleers1, W. Van Den Bogaert2, J. Wuestenbergs2, W. Van de Voorde2, E. Cuypers1
1KU Leuven Toxicology and Pharmacology, Campus Gasthuisberg, Leuven, Belgium; 2KU Leuven, Imaging and Pathology Department, Division Forensic Biomedical Sciences, University of Leuven (KU Leuven), Campus Sint-Rafaël, Leuven, Belgium
P 174The performance of Immobilized Artificial Membrane (IAM) chromatography to model ecotoxicity of pesticides
F. Tsopelas1, C. Stergiopoulos1, L.A. Tsakanika, M. Ochsenkühn-Petropoulou1, A. Tsantili-Kakoulidou2
1Laboratory of Inorganic and Analytical Chemistry, School of Chemical Engineering, National Technical University of Athens, Greece; 2Department of Pharmaceutical Chemistry, School of Pharmacy, National and Kapodistrian University of Athens, Greece
P 175Simultaneous analysis of 20 unauthorized pharmaceutical compounds associated with treating senile diseases illegally adulterated in dietary supplements using UPLC-UV
S. Park, N.S. Kim, H.S. Choi, J.H. Kim, S.H. Moon, H. Kang
Division of Advanced Analysis, National Institute of Food and Drug Safety Evaluation
P 176Using MS/MS All with SWATH® acquisition for detection of fentanyl analogs and synthetic opioids in real hair samples with the SCIEX X500R QTOF system
D. Di Corcia, A. Salomone, E. Gerace, M. Vincenti
Centro Regionale Antidoping “A. Bertinaria”, Turin, Italy; Dipartimento di Chimica Analitica, Università degli Studi di Torino, Italy
P 177Organophosphate intoxication: Improvement of biological barriers penetration of AChE reactivators by encapsulation with cucurbit(7)uril
R. Andrýs1, A. Klusoňová1, M. Lísa1, J. Žďárová-Karasová2
1Department of Chemistry, Faculty of Science, University of Hradec Králové, Czech Republic; 2Department of Toxicology and Military Pharmacy, Faculty of Military Health Sciences Hradec Králové, University of Defence, Praha, Czech Republic
P 178Extraction and Quantification of Synthetic Cathinones From Human Urine using SOLA™ SPE and HILIC-MS/MS
M.R. Franklin, K. Woodmansey
Thermo Fisher Scientific, Tudor Road, Runcorn, United Kingdom

Applications - Metabolomics and lipidomics

P 179RAPID ANALYTICAL PLATFORMS FOR BIOFLUID PROFILING IN DISCOVERY METABOLOMICS
A.M. King1,2, L.G. Mullin3, I.D. Wilson4, P.D. Rainville3, R.S. Plumb3, L.A. Gethings1, G. Maker2, R. Trengove2
1Waters Corporation, Wilmslow, Cheshire, UK; 2Separations Science and Metabolomics laboratory, Murdoch
University, Murdoch, WA, Australia;
3Waters Corporation, Milford, USA; 4Computational and Systems Medicine, Department of Surgery and Cancer, Faculty of Medicine, Imperial College London, UK
P 180SPENT MEDIA MONITORING BY LIQUID CHROMATOGRAPHY COUPLED TO CHARGED AEROSOL DETECTOR (LC-CAD)
M.R. da Silva, I. Zaborowska, S. Carillo, J. Bones
NIBRT - National Institute for Bioprocessing Research & Training, Dublin, Ireland
P 181COMPLEMENTARY ANALYSIS OF POLAR AND NON-POLAR LIPIDS BY HEART-CUT TWO-DIMENSIONAL LIQUID CHROMATOGRAPHY COUPLED TO MASS SPECTROMETRY
C.M. Wienken, H. Hayen
Institute of Inorganic and Analytical Chemistry, University of Münster, Münster, Germany
P 182PHYTOCHEMICAL CHARACTERIZATION OF PROTEA PURE AND HYBRID CULTIVARS WITH THE AID OF LIQUID CHROMATOGRAPHY-ION MOBILITY MASS SPECTROMETRY (LC-IMMS) AND THE APPLICATION OF METABOLOMICS TOOLS IN DETERMINING POSSIBLE METABOLITES LINKED TO LEAF BLACKENING
K. Masike1, A. de Villiers2, M. Stander1,3
1Department of Biochemistry, University of Stellenbosch, Matieland, South Africa; 2Department of Chemistry and Polymer Science, University of Stellenbosch, Matieland, South Africa; 3Central Analytical Facility, Stellenbosch University, Matieland, South Africa
P 183CE-MS-BASED PLASMA METABOLOMICS FOR THE DISCOVERY OF BIOMARKERS FROM A MOUSE MODEL FOR ACUTE EPILEPTIC SEIZURES
K. Segers1,2, W. Zhang3, D. Mangelings1, T. Hankemeier3, D. De Bundel2, Y.V. Heyden1, I. Smolders2, R. Ramautar3, A. Van Eeckhaut2
1Department of Analytical Chemistry, Applied Chemometrics and Molecular Modelling, Vrije Universiteit Brussel, Belgium; 2Department of Pharmaceutical Chemistry, Drug Analysis and Drug Information, Center for Neurosciences (C4N), Vrije Universiteit Brussel, Belgium; 3Division of Systems Biomedicine and Pharmacology, Leiden Academic Centre for Drug Research (LACDR), Leiden University, The Netherlands
P 184HIGH-THROUGHPUT COMPREHENSIVE COVERAGE OF HYDROPHILIC AND HYDROPHOBIC METABOLITES IN BEER UTILIZING A DUAL SEPARATION/HIGH RESOLUTION ACCURATE MASS SPECTROMETRY SYSTEM
I. Ntai, M. Samonig, S. Samra, A. Souza, C. Paul, A. Huhmer
Thermo Fisher Scientific, San Jose, CA, USA; Thermo Fisher Scientific, Germering, Germany
P 185METABOLITE PROFILING OF YEAST CELLS BY MEANS OF CAPILLARY ION CHROMATOGRAPHY-MASS SPECTROMETRY
H. Schöttler, H. Hayen
Institute of Inorganic and Analytical Chemistry, University of Muenster, Germany
P 186GRAPHITIZED CARBON BLACK ENRICHMENT PROCEDURE FOR THE DETECTION OF PHOSPHOLIPIDS IN OLIVE OIL BY LIQUID CHROMATOGRAPHY-HIGH RESOLUTION MASS SPECTROMETRY
C.M. Montone, M. Antonelli, B. Benedetti, A. Cerrato, A. Laganà
Department of Chemistry, Sapienza Università di Roma, Italy
P 187THE METABONOMIC PROFILING OF CHICKEN EGGS DURING STORAGE USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY QUADRUPOLE TIME-OF-FLIGHT MASS SPECTROMETRY
A.E. Johnson, K.L. Sidwick, V.R. Pirgozliev, D.F. Thompson
PhD student, Chemical and Physical Sciences, Keele University, Newcastle-under-Lyme, Staffordshire
P 188QUANTIFICATION OF POLAR METABOLITES IN URINE USING AUTOMATED PARALLEL DERIVATIZATION AND LC-SWATH/MS
M.F. Cifuentes Girard1, D. Ruskic1, R. Picenoni2, G. Boehm2, G. Hopfgartner1
1Life Sciences Mass Spectrometry, Department of analytical and Inorganic Chemistry, University of Geneva, Switzerland; 2CTC Analytics AG, Zwingen, Switzerland
P 189Evaluation of antidepressant-like activity of kiwifruit (Actinidia deliciosa) extract on mouse models of depression
C.M. Marzo, M. Bianconi, C. Chiamulera, F. Guzzo
University of Verona, Department of Biotechnology, Verona, Italy; University of Verona, Department of Biotechnology, Verona, Italy; University of Verona, Department of Diagnostic and Public Health, Verona, Italy; University of Verona, Department of Biotechnology, Verona, Italy
P 190METABOLOMICS-ASSISTED METABOLITE PROFILING OF SEONGSANAMIDE A IN HUMAN LIVER MICROSOMES
K.H. Liu1, Z. Wu1, G.H. Lee1, H. Choi2
1BK21 Plus KNU Multi-Omics based Creative Drug Research Team, College of Pharmacy and Research Institute of Pharmaceutical Sciences, Kyungpook National University, Korea; 2College of Pharmacy, Yeungnam University, Korea
P 191THE DETECTION OF REMAINS IN WATER - SMALL MOLECULE PROFILING
M. Williams, F. Drijfhout
Keele University
P 192Comprehensive UHPLC-MS/MS lipidomics profiling to study effects of betulin on keratinocytes
C. Calderón, I. Merfort, M. Lämmerhofer
Institute of Pharmaceutical Sciences, Pharmaceutical (Bio-)Analysis, University of Tübingen, Germany; Institute of Pharmaceutical Sciences, Department of Pharmaceutical Biology and Biotechnology, University of Freiburg, Germany; Institute of Pharmaceutical Sciences, Pharmaceutical (Bio-)Analysis, University of Tübingen, Germany
P 193Lipidomic analysis and comparison of human body fluids and exosomes by various MS techniques
O. Peterka1, R. Jirásko1, M. Chocholoušková1, D. Wolrab1, L. Kuchař2, M. Holčapek1
1University of Pardubice, Faculty of Chemical Technology, Department of Analytical Chemistry, Pardubice, Czech Republic; 2Research Unit for Rare Diseases, Department of Pediatrics and Adolescent Medicine, First Faculty of Medicine, Charles University and General University Hospital in Prague, Czech Republic
P 194Challenges in the structural elucidation of Gram-negative bacterial lipid A molecules applying HPLC-MS/MS
Á. Dörnyei1, V. Sándor2, A. Kilár2, B. Kocsis3, F. Kilár1,2
1Department of Analytical and Environmental Chemistry, and Szentágothai Research Centre, University of Pécs, Hungary; 2Institute of Bioanalysis, Medical School, University of Pecs, Hungary; 3Department of Medical Microbiology and Immunology, Medical School, University of Pécs, Hungary
P 195Rapid analysis of selected neurotransmitters using hydrophilic interaction liquid chromatography
M. Alsaeedi1,2, A.M. Hogan1,2, J.D. Glennon1,2
1Innovative Chromatography Group, Irish Separation Science Cluster (ISSC); 2School of Chemistry and the Analytical, Biological and Chemistry Research Facility (ABCRF), University College Cork, Ireland
P 196Fecal oxo-bile acid composition evaluated by HPLC-ESI-MS/MS in mice model of experimental ulcerative colitis
E. Porru1, J. Fiori1, N. McGinley2, S. Keely2, A. Roda1
1Department of Chemistry “G. Ciamician”, Alma Mater Studiorum University of Bologna, Italy; 2Royal College of Surgeons in Ireland, Education and Research Centre, Beaumont Hospital, Dublin, Ireland
P 197Functional characterization of the human gut microbiota
V. Vidová, G. Dovrtělová, K. Coufalíková, E. Stuchlíková, A.-C. Aust, J. Klanová, Z. Spáčil
RECETOX, Faculty of Science, Masaryk University, Brno, Czech Republic
P 198Lipidomic changes in patients with nonalcoholic fatty liver disease (NAFLD) depending on histological severity
Y. Jung1,2, W. Kim3, G.S. Hwang1,4
1Integrated Metabolomics Research Group, Western Seoul Center, Korea Basic Science Institute, Seoul, Republic of Korea; 2Department of Life Science, Ewha Womans University, Seoul, Republic of Korea; 3Division of Gastroenterology and Hepatology, Department of Internal Medicine, Seoul National University College of Medicine, Seoul Metropolitan Government Boramae Medical Center, Seoul, Republic of Korea; 4Department of Chemistry and Nano Science, Ewha Womans University, Seoul, Republic of Korea
P 199Towards greater metabolite coverage – use of parallel LC-MS to cover analysis of metabolites and lipids in a single instrument setup
M. Witting1,2, G. Greco3, D. Hemmler1, F. Steiner3, P. Schmitt-Kopplin1,2
1Research Unit Analytical BioGeoChemistry, Helmholtz Zentrum München, Neuherberg, Germany; 2Chair of Analytical Food Chemistry, Technische Universität München, Freising, Germany; 3Thermo Fisher Scientific, Germering, Germany
P 200Development and validation of an LC-MS/MS method for determination of vitamin B-group and some its derivatives in whole blood
D. Kahoun1, P. Fojtíková1, E. Nováková2, V. Hypša2
1Institute of Chemistry, Faculty of Science, University of South Bohemia in Ceske Budejovice, Ceske Budejovice, Czech Republic; 2Institute of Parasitology, Faculty of Science, University of South Bohemia in Ceske Budejovice, Ceske Budejovice, Czech Republic

Applications - Natural products

P 201INVESTIGATION OF FREE AMINO ACID PROFILE AND ANTIOXIDANT EFFECTS OF EGG CHALAZA AFTER ENZYMATIC HYDROLYZATION
S.Y. Wang1, J.K. Tseng2,3, C.J. Chan1, Y.L. Lin1, J.W. Chen1,4, D.J. Yang5, Y.C. Chen1
1Department of Animal Science and Technology, National Taiwan University, Taipei, Taiwan; 2Department of Optometry, Asia University, Taichung, Taiwan; 3Department of Medical Research, China Medical University Hospital, China Medical University, Taichung, Taiwan; 4Poultry Industry Section, Department of Animal Industry, Council of Agriculture, Executive Yuan, Taipei, Taiwan; 5Institute of Food Safety and Health Risk Assessment, National Yang-Ming University, Taipei, Taiwan
P 202ANALYSIS OF STEROID HORMONES IN SHELLED EGGS FROM LAYER BREEDS COMMON TO TAIWAN BY LIQUID CHROMATOGRAPHY-TANDEM MASS SPECTROMETRY
S.Y. Wang1, I.C. Li2, W.Y. Yang3, C.H. Chou2, Y.C. Chen1, S.L. Kuo4
1Department of Animal Science and Technology, National Taiwan University, Taipei, Taiwan; 2Zoonoses Research Center and School of Veterinary Medicine, National Taiwan University, Taipei, Taiwan; 3Department of Basic Science, College of Veterinary Medicine, Mississippi State University, USA; 4Technical Service Center, National Animal Industry Foundation, Pingtung, Taiwan
P 203INHIBITORY EFFECTS OF PATENT ACTIVE WATER ON PARTICULATE MATTER 2.5 UM (PM2.5)-INDUCED LESION IN ENDOTHELIAL CELLS
S.L. Peng1, Y.W. Liu2, C.J. Weng2
1Institute of Medicine. Chung Shan Medical University, Taichung, Taiwan; 2Master Degree Program of Applied Living Science in Department of Living Services Industry, Tainan University of Technology, Taiwan
P 204DEVELOPMENT OF NEW APPROACHES FOR DETERMINATION AND IDENTIFICATION OF COMPONENTS FROM PLANT MATERIALS USING HPLC-MS DATA
P. Turova, I. Rodin, A. Stavrianidi
Faculty of Chemistry, Lomonosov Moscow State University, Moscow, Russia
P 205OBTAINING AND ANALYSING TROPICAL FLOWER COLORANTS FOR THEIR USE IN TEMPERA PAINTINGS
E.E. Stashenko, L. Sierra, R. González, J. Mejía, Y. Córdoba, J.R. Martínez
Chromatography and Mass Spectrometry Research Centre, CROM-MASS-CENIVAM. Universidad Industrial de Santander, Bucaramanga, Colombia
P 206NEW TOOLS FOR OAK SPECIES IDENTIFICATION
G. Buche1, C. Colas1, L. Fougère1, T. Giordanengo2, E. Destandau1
1Univ. Orléans, CNRS, ICOA, UMR 7311, Orléans, France; 2R&D Tonnellerie Radoux, Jonzac, France
P 207DETERMINATION OF PHYTOBIOTICS BY REVERSED-PHASE ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY FROM COMPLEX MATRIX
J. Rico-Ruiz, A. Casarín-Valverde, M. Olvera-Garcia, H. Leyva-Jimenez, L. Gómez-Osorio, G. Villar-Patiño
Euro-Nutec, México
P 208Identification of some polyphenols from aerial parts extracts of Daucus carota by LCMS
H. Kadri1, S.E. Djilani1, A. Djilani1, A. Dicko2
1LSBO, Badji Mokhtar University, Annaba, Algeria; 2LCME, Lorraine University, Metz, France
P 209SENSITIVE AND SELECTIVE ANALYSIS OF WOOD SUGARS AND URONIC ACIDS FOR BIOFUEL RESEARCH WITH ELECTROCHEMICAL DETECTION
K. Folmert, K. Monks
KNAUER Wissenschaftliche Geräte GmbH, Berlin, Germany
P 210Development of HPLC/MS/MS method for analysis of biologically active compounds in Tropaeolum majus
L. Česlová, T. Šalomounová
Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Czech Republic
P 211Comparative study of four extraction methods of Lawsonia inermis harvested from two different regions in Morocco and their HPLC-MS analysis
F.Z. Alem, G.S. Ananthabhotla, A.L.Oliveira, Z. Charrouf, B. Rhourri-Frih
Faculty of Pharmacy-University of Bordeaux CGFB - CBMN - UMR 5248, Bordeaux, France; Universidade de São Paulo, Pirassununga, Brazil; Faculty of Sciences, Mohammed V, Rabat, Morocco; Narayana Pharmacy College, Jawaharlal Nehru Technological University Anantapur, India
P 212Chemical composition determined by UHPLC-ESI-Orbitrap-MS and evaluation of the antioxidant potential Lippia origanoides extracts before and after plant hydrodistillation
J.J. Mejía Medina1, Y. Córdoba Campo1, L.J. Sierra1, A.J. Arias1, A. Kumara2, J.R. Martínez1, E.E. Stashenko1
1Research Center for Chromatography and Mass Spectrometry, CIBIMOL-CENIVAM, Universidad Industrial de Santander, Colombia; 2Department of Chemistry, Hemwati Nandan Bahuguna Garhwal University, Uttarakhand, India
P 213ionic liquid-based surfactants for the extraction of natural compounds from Vitis Vinifera leaves
G. Mastellone1,2, I. Pacheco-Fernández2, C. Cagliero1, V. Pino2,3
1Dipartimento di Scienza e Tecnologia del Farmaco, Universtà degli Studi di Torino, Italy; 2Departamento de Química, Unidad Departamental de Química Analítica, Universidad de La Laguna, Tenerife, Spain; 3University Institute of Tropical Diseases and Public Health, Universidad de La Laguna, Tenerife, Spain
P 214The simultaneous determination of six furocoumarin markers in citrus oil by UPLC coupled with UV and MS detection
Z. Li, P. Hong, P. McConville
Waters
P 215HPLC-UV Method Development for Baseline Resolution of 17 Cannabinoids
M. Wilcox, E. Franklin, S. Anderson
Regis Technologies, Inc, Morton Grove, Illinois, USA
P 216Comprehensive two-dimensional analysis of saponins from Quillaja Saponaria: comparison of RPLCxRPLC and HILICxRPLC modes
N. Biedermann1,2, S. Nuccio2, R. de Vaumas2, K. Faure1
1Institut des Sciences Analytiques, CNRS, Université Lyon, Villeurbanne, France; 2Extrasynthèse, Genay, France
P 217CHARACTERIZATION OF A STANDARDIZED EXTRACT OF FRESH CURCUMA LONGA RHIZOMES
V. Bardot1, A. Leblanc1, L. Berthomier2, I. Ripoche2, M. Leremboure2, Pierre Chalard2, Michel Dubourdeaux1
1Groupe Pileje, Paris, France; 2Université Clermont Auvergne, CNRS, SIGMA Clermont, Clermont-Ferrand, France
P 218Use of bioactives from agrifood by-products in topical cosmesic hydrogels
C. Stevanin1, E. Esposito1, R. Cortesi1, M. Sguizzato1, R. Voltan2, N. Marchetti1
1Department of chemical and pharmacologicall sciences, University of Ferrara, Italy; 2Department of morphology, surgery and medicine

Applications - Other

P 219DISPOSABLE ZIRCONIUM-GOLD ELECTRODE FOR SENSITIVE ELECTROCHEMICAL DETECTION IN HPLC
M.A. Islam1, P. Mahbub1,2, M. Macka1,3,4, B. Paull1,5
1Australian Centre for Research on Separation Science (ACROSS), School of Natural Sciences, University of Tasmania, Hobart, Australia; 2Institute for Sustainable Industries and Liveable Cities, Victoria University, Melbourne, Australia; 3Department of Chemistry and Biochemistry, Mendel University in Brno, Czech Republic; 4Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic; 5ARC Training Centre for Portable Analytical Separation Technologies (ASTech), School of Natural Sciences, University of Tasmania, Hobart, Australia
P 220Analysis and evaluation of Monosaccharide composition and by-products in various cellulose nanofibers(CNFs) by HPLC
A. Morita, Y. Toyota, M. Kawashima, Y. Hirao
Shimadzu Corporation
P 221HPLC COLUMNS WITH UNIQUE SELECTIVITY FOR NUCLEIC ACIDS
A. Levine, T. Ono, T. Hirose, D. Keck
Nacalai USA, San Diego, USA; Nacalai USA, San Diego, USA; Nacalai Tesque Inc., Kyoto, Japan; Nacalai Tesque Inc., Kyoto, Japan
P 222Chromatographic behaviour of newly prepared double-labeled oligonucleotide probes containing azaphthalocyanines dye and different fluorophores
R. Kučera, A. Sčensná, M. Miletín, P. Zimčík
Charles University, Faculty of Pharmacy in Hradec Králové, Dept. of Pharmaceutical Chemistry and Pharmaceutical Analysis, Czech Republic
P 223Bioactive polymer lubricant unconsciously administered into the body
K. Naumoska1, U. Jug1,2, V. Metličar1,2, I Vovk1
1Department of Food Chemistry, National Institute of Chemistry, Ljubljana, Slovenia; 2Faculty of Chemistry and Chemical Technology, University of Ljubljana, Slovenia
P 224Polymeric lubricant, an usually overlooked interference in analytical studies and bioassays
U. Jug1,2, K. Naumoska1,3, V. Metličar1,2, A. Schink3, D. Makuc4, I. Vovk1, J. Plavec1,4,5, K Lucas3
1Department of Food Chemistry, National Institute of Chemistry, Ljubljana, Slovenia; 2Faculty of Chemistry and Chemical Technology, University of Ljubljana, Ljubljana, Slovenia; 3Multiphase Chemistry Department, Max Planck Institute for Chemistry, Mainz, Germany; 4Slovenian NMR Centre, National Institute of Chemistry, Ljubljana, Slovenia; 5EN-FIST Centre of Excellence, Ljubljana, Slovenia
P 225Towards the identification of the lichen species in historical orchil dyes by HPLC-MS/MS
F. Gosetti1, E. Calà1, M. Benzi1, M. Gulmini2, A. Idone2, I. Serafini3,4, A. Ciccola4, R. Curini3, I. Whitworth5, M. Aceto1
1Department of Science and Technological Innovation, University of Piemonte Orientale, Alessandria, Italy; 2Department of Chemistry, University of Torino, Italy; 3Department of Chemistry, University of Roma "La Sapienza", Italy; 4Department of Chemistry and Pharmaceutical Technologies, University of Roma "La Sapienza", Italy
P 226Optimising lipid separation for cultural heritage applications
J. Keute, T. Devièse
Research Lab for Archaeology and the History of Art, University of Oxford, United Kingdom
P 227Determination of Fluoride in LiPF6-based Lithium Ion Battery Electrolytes by Column-Switching Ion Chromatography
V. Kraft, P. Jochems, R. Ludwig, G.J. Schad
Shimadzu Europa GmbH, Duisburg, Germany
P 228Fast and Easy Injection of Large Sample Volumes in Preparative HPLC
F. Rieck
Agilent Technologies, Inc., Waldbronn, Germany

Applications - Proteomics

P 229EXOSOMES, A CHALLENGING TARGET FOR QUANTITATIVE PROTEOMICS
D. Hixson, M. Begić, M. Šrajer Gajdošik, A. Kovač Peić, H. Callanan, J. Aliotta, K. Brilliant, P. Quesenberry, D. Josić
Warren Alpert Medical School, Brown University, Providence, USA
P 230FAST SCREENING AND DETERMINATION OF BINDING CONSTANTS FOR BETTER UNDERSTANDING THE INTERACTION BETWEEN IRON (III) ION AND METALLOPROTEINS, TRANSFERRIN AND APOTRANSFERRIN, BY USING AFFINITY CAPILLARY ELECTROPHORESIS
H.A Alhazmi
1Department of Pharmaceutical Chemistry, College of Pharmacy, Jazan University, Saudi Arabia; 2Substance Abuse Research Centre, Jazan University, Saudi Arabia
P 231SERUM PROTEOMICS ANALYSIS OF PSYCHIATRIC DISORDERS BY NANO-LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY
E.C. Santa Cruz, F. Zandonadi, A. Sussulini
Laboratory of Bioanalytics and Integrated Omics (LaBIOmics), Departamento de Química analítica, Universidade de Campinas, UNICAMP, Campinas, Brazil
P 232ONE-STEP MALTOSE-FUNCTIONALIZATION OF MAGNETIC NANOPARTICLES USING SELF-ASSEMBLED OLIGOPEPTIDES FOR HIHGLY EFFICIENT ENRICHMENT OF GLYCOPEPTIDES
L. Zhang, F. Dang
Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, School of Chemistry and Chemical Engineering, Shaanxi Normal University, China
P 233LC-MS/MS CHARACTERIZATION OF THE PROTEIN FRACTION IN WASTE PRODUCTS FROM THE TUNA FISHERY INDUSTRY
P. Arena1, P. Donato2, D. Donnarumma3, P. Dugo1,3,4, L. Mondello1,3,4
1Dipartimento di Scienze Chimiche, Biologiche, Farmaceutiche ed Ambientali, University of Messina, Italy; 2Dipartimento di Scienze Biomediche, Odontoiatriche e delle Immagini Morfologiche e Funzionali, University of Messina, Italy; 3Chromaleont s.r.l., c/o Dipartimento di Scienze Chimiche, Biologiche, Farmaceutiche ed Ambientali, University of Messina, Italy; 4Department of Medicine, University Campus Bio-Medico of Rome, Italy
P 234ANALYTICAL STRATEGY FOR THE METAPROTEOMIC INVESTIGATION OF ATMOSPHERIC BIOAEROSOL FOR ENVIRONMENTAL ANALYSIS
M. Antonelli, B. Benedetti, A. Cerrato, C. Montone, A. Laganà, A.L. Capriotti
Department of Chemistry - La Sapienza, University of Rome, Italy
P 235Effect of defatting and solvents selection on the extraction of allergen proteins from almonds
L. Burdějová, F. Duša, D. Moravcová
Institute of Analytical Chemistry Czech Academy of Sciences, Brno, Czech Republic
P 236plasma exosomal proteomics in lung cancer patients
J. Shin, J. Lee, M. Jeong, W. Cho*
Department of Chemistry, Wonkwang University, Iksan, Jeonbuk, South Korea
P 237Development of a protocol for skin protein extraction towards a liquid chromatography-mass spectrometry-based proteomics approach to evaluate skin wound age in forensic medicine
M. Mattarozzi1, G. Paredi2, G. Vignali3, S. Tambuzzi3, M. Milioli4, C. Cattaneo3, A. Mozzarelli5, M. Careri1
1Department of Chemistry, Life Sciences and Environmental Sustainability, University of Parma, Italy; 2Interdepartmental Center SITEIA.PARMA, University of Parma, Italy; 3Institute of Legal Medicine, Department of Biomedical Sciences for Health, University of Milan, Italy; 4Preclinical Pharmacokinetics, Biochemistry and Metabolism Department, Chiesi Farmaceutici SpA, Parma, Italy; 5Department of Food and Drug, University of Parma, Italy

Applications - Sample preparation

P 238EXTRACTION OF ANTISENSE OLIGONUCLEOTIDES USING MICROEXTRACTION BY PACKED SORBENT
Ł. Nuckowski, A. Kaczmarkiewicz, S. Studzińska
Nicolaus Copernicus University, Toruń, Poland
P 239MECHANOCHEMICAL SOLID PHASE EXTRACTION WITH ENRICHMENT CAPABILITY FOR ENVIRONMENTAL CONTAMINANTS IN PLANTS
Y. Zhou, W. Bi, D. Da Yong Chen
Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, Jiangsu Key Laboratory of Biomedical Materials, College of Chemistry and Materials Science, Nanjing Normal University, China
P 240SYNTHESIS OF A SORBENT BASED ON SILICA GEL FUNCTIONALIZED WITH IONIC LIQUID AND HPLC-ELSD METHOD FOR DETERMINATION OF FATTY ACIDS IN BREAST MILK.
M. Pajewska-Szmyt1,2, R. Gadzała-Kopciuch1,2, B. Buszewski1,2
1Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University in Toruń, Poland; 2Interdisciplinary Centre for Modern Technologies, Nicolaus Copernicus University, Toruń, Poland
P 241A SOLIDIFICATION OF SWITCHABLE SOLVENT-BASED QUECHERS METHOD FOR DETECTION OF PESTICIDES IN FRUITS AND VEGETABLES
J. Wang, H.L. Duan, S.Y. Ma, H.Y. Zhan, Z.Q. Zhang*
School of Chemistry & Chemical Engineering, Shaanxi Normal University, Xi’an, China
P 242CYCLODEXTRINS AND THEIR APPLICATION IN ENVIRONMENTAL ANALYSIS: VARIABLES AFFECTING HOST-GUEST INTERACTION WITH DIFFERENT TYPES OF ANALYTES
C. Belenguer-Sapiña1, E. Pellicer-Castell1, E.F. Simó-Alfonso1, P. Amorós2, A. Mauri-Aucejo1
1Department of Analytical Chemistry, Faculty of Chemistry, University of Valencia, Burjassot, Spain; 2Institute of Materials Science, University of Valencia, Spain
P 243NANOFIBROUS SORBENT FOR DIRECT EXTRACTION OF NON-STEROIDAL ANTI-INFLAMMATORY DRUGS FROM HUMAN SERUM
H. Raabová1, M. Háková1, L. Chocholoušová Havlíková1, D. Šatínský1, J. Chvojka2, J. Erben2
1The Charles University, Faculty of Pharmacy, Department of Analytical Chemistry, Akademika Heyrovského, Hradec Králové, Czech Republic; 2The Technical University of Liberec, Faculty of Textile Engineering, Department of Nonwovens and Nanofibrous Materials, Liberec, Czech Republic
P 244DETERMINATION OF AFLATOXINS IN MEDICINAL HERBS BY USING MESOPOROUS SILICA-STRUCTURED MATERIALS FOR ITS PRECONCENTRATION
E. Pellicer-Castell1, C. Belenguer-Sapiña1, A. Mauri-Aucejo1, P. Amorós2, J.M. Herrero-Martínez1
1Department of Analytical Chemistry, Faculty of Chemistry, University of Valencia, Burjassot, Spain; 2Institut of Material Science (ICMUV), University of Valencia, Paterna, Spain
P 245MAGNETIC DISPERSIVE SOLID PHASE EXTRACTION BASED ON A NOVEL ADSORBENT FOR THE DETECTION OF PHENOLIC ACIDS COMPOUNDS IN FRUIT WINE BY HPLC-MS/MS
C.Y. Kang, W.T. Jung, H.L. Lee
Department of Chemistry, Fu Jen Catholic University, New Taipei, Taiwan
P 246MULTI-RESIDUE METHOD FOR THE DETERMINATION OF VETERINARY DRUGS IN SEDIMENT FROM AQUACULTURE
N. Tetzner, S. Rath
Institute of Chemistry, Department of Analytical Chemistry, University of Campinas, Campinas, Brazil
P 247A NANOPOROUS CARBON DERIVED FROM BIMETALLIC ORGANIC-FRAMEWORK FOR MAGNETIC SOLID-PHASE EXTRACTION OF BISPHENOL ANALOGS
S.Y. Ma, X. Wang, H.L. Duan, J. Wang, H.Y. Zhan*, Z.Q. Zhang*
Shaanxi Normal University, Xi’an, China
P 248FPSE-HPLC-PDA METHOD FOR RAPID DETERMINATION OF SOLAR UV FILTERS IN HUMAN WHOLE BLOOD, PLASMA AND URINE
A. Tartaglia1, M. Locatelli1, K.G. Furton2, E. Sperandio1, S. Ulusoy3, H.I. Ulusoy3, A. Kabir2
1Department of Pharmacy, University of Chieti–Pescara “G. d’Annunzio”, Chieti, Italy; 2Department of Chemistry and Biochemistry, International Forensic Research Institute, Florida International University, Miami, USA; 3Department of Analytical Chemistry, Faculty of Pharmacy, Cumhuriyet University, Sivas, Turkey
P 249Optimization of sample preparation for sp-carbon linear chains SPE-based concentration
A. Facibeni, S. Peggiani, D. Giribone, C.S. Casari
Energy Dept., Politecnico di Milano, Italy; Energy Dept., Politecnico di Milano, Italy; Analytical Consultant, Milan, Italy; Energy Dept., Politecnico di Milano, Italy
P 250MINIATURIZATION OF MONOLITHIC IMMOBILIZED ENZYMATIC REACTORS SYNTHESIZED IN SITU AND COUPLED ON-LINE WITH NANO-LIQUID CHROMATOGRAPHY
S. Perchepied1, N. Delaunay1, V. Pichon2
1Department of Analytical, Bioanalytical Sciences, and Miniaturization - UMR CBI 8231, ESPCI Paris, PSL University, Paris, France; 2Department of Analytical, Bioanalytical Sciences, and Miniaturization, UMR CBI 8231 - Sorbonne University, Paris, France
P 251IN SITU SYNTHESIS OF A MOLECULARLY IMPRINTED MONOLITH IN A CAPILLARY AND ON-LINE COUPLING TO NANO-LIQUID CHROMATOGRAPHY FOR THE SELECTIVE EXTRACTION OF COCAINE AND ITS METABOLITE FROM BIOLOGICAL FLUIDS
T. Bouvarel1, N. Delaunay1, V. Pichon2
1Department of Analytical, Bioanalytical Sciences, and Miniaturization, UMR CBI 8231, ESPCI Paris, PSL University, Paris, France; 2Department of Analytical, Bioanalytical Sciences, and Miniaturization, UMR CBI 8231, ESPCI Paris, PSL University - Sorbonne University, Paris, France
P 252ALGINATE/AMINE-FUNCTIONALIZED MIL-101(CR)/MAGNETITE HYDROGEL COMPOSITE FOR THE MAGNETIC SOLID-PHASE EXTRACTION AND LIQUID CHROMATOGRAPHY-TANDEM MASS SPECTROMETRY ANALYSIS OF POLAR CHLOROPHENOXY ACID HERBICIDES
S.C. Tan1, H. Kee Lee2
1NUS Graduate School for Integrative Sciences and Engineering, National University of Singapore, University Hall, Singapore; 2Department of Chemistry, National University of Singapore, Singapore
P 253APPLYING NON-TRADITIONAL EXTRACTION PLATFORMS FOR EFFICIENT PHARMACEUTICAL SAMPLE PREPARATION
C. Wang, J. Yeh
Gilead Sciences Inc., Foster City (CA)
P 254DEVELOPMENT OF AN IMMOBILIZED-TRYPSIN REACTOR COUPLED TO LIQUID CHROMATOGRAPHY AND MASS SPECTROMETRY (LC-MS) FOR THE ANALYSIS OF LONG-LIVED ADDUCTS OF SULFUR MUSTARD WITH HEMOGLOBIN IN BLOOD SAMPLES
F. Hallez1, A. Combès1, A. Bossée2, C. Desoubries2, V. Pichon1,3
1Department of Analytical, Bioanalytical Sciences and Miniaturization (LSABM), UMR CBI 8231 CNRS – ESPCI Paris, PSL University, Paris, France; 2DGA CBRN Defence, Analytical Chemistry, Vert-le-Petit, France; 3Sorbonne University, Paris, France
P 255SWEET, SWEETER STEVAI - THE WHOLE STORY FROM ANALYTICAL METHOD DEVELOPMENT TO A ROBUST AND EFFECTIVE ONLINE SPE PURIFICATION OF STEVIOGLYCOSIDES WITH PREPARATIVE HPLC
K. Monks, Y. Krauke, J. Böttcher, J. Menke
KNAUER Wissenschaftliche Geräte GmbH
P 256DYNAMIC MIXING DROPLET DISPERSION LIQUID-LIQUID MICRO-EXTRACTION (DMD-DLLME) COMBINED WITH HIGH PERFORMANCE LIQUID CHROMATOGRAPHIC METHOD FOR THE DETERMINATION OF PYRETHROIDS IN AQUEOUS SAMPLES
Y.H. Liu, Y. Wang, Y.Y. Shu*
Department of Chemistry, National Kaohsiung Normal University, Kaohsiung, Taiwan
P 257SIMPLIFIED SAMPLE PREPARATION OF NITROFURANTOIN ORAL SUSPENSION FOR HPLC ANALYSIS
S. Avramov1, T. Boyanov2, A. Valev2
1Chromassist Ltd, Sofia, Bulgaria; 2Bulgarian Scientific Pharmaceutical Laboratory at the Erudita Pharmacy of Pediatrics, Sofia, Bulgaria
P 258MANIPULATION OF THE CLOUD POINT TEMPERATURE OF TRITON X-100 AND ITS APPLICATION FOR THE CLOUD POINT EXTRACTION AND HIGH PERFORMANCE LIQUID CHROMATOGRAPHY – MASS SPECTROMETRIC QUANTIFICATION OF VETERINARY MEDICINE MACROLIDES
H. Iwahashi1, W.L. Hinze2, Y. Takagai1
1Faculty of Symbiotic Systems Science, Cluster of Science and Technology, Fukushima University, Fukushima, Japan; 2Department of Chemistry, Wake Forest University, Winston-Salem, USA
P 259Simultaneous extraction method for the analysis of water- and fat-soluble vitamins
Ž. Temova Rakuša, A. Kristl, R. Roškar
University of Ljubljana, Faculty of Pharmacy, Ljubljana, Slovenia
P 260Application of conventional MISPE, pipette tip MISPE and magnetic MIP extraction in wine analysis
K. Hroboňová, A. Špačková, M. Ondáková
Slovak University of Technology in Bratislava, Faculty of Chemical and Food Technology, Institute of Analytical Chemistry, Bratislava, Slovak Republic
P 261Automated salting-out liquid-liquid extraction in Lab-In-Syringe coupled to online SPE and HPLC for the determination of sulfonamides in urine
K. Fikarová, B. Horstkotte, H. Sklenářová, P. Solich
Department of Analytical Chemistry, Faculty of Pharmacy in Hradec Králové, Charles University, Akademika Heyrovského, Czech Republic
P 262Determination of primary aromatic amines from olive oil for testing their migration from food contact materials
B.S. Szabó, C. Kirchkeszner, N. Petrovics, Z. Nyiri, Z. Eke
Hevesy György PhD School of Chemistry, Eötvös Loránd University, Budapest, Hungary; Hevesy György PhD School of Chemistry, Eötvös Loránd University, Budapest, Hungary; Hevesy György PhD School of Chemistry, Eötvös Loránd University, Budapest, Hungary; Joint Research and Training Laboratory on Separation Techniques, Eötvös Loránd University, Budapest, Hungary; Joint Research and Training Laboratory on Separation Techniques, Eötvös Loránd University, Budapest, Hungary
P 263Use of salting-out assisted liquid-liquid extraction (SALLE) for the analysis of tyrosine kinase inhibitors (TKIs) in human plasma by capillary electrophoresis (CE) and liquid chromatography (LC)
O.S. Ahmed1,3, Y. Ladner1, J. Montels1, L. Philibert2, C. Perrin1
1Institut des Biomolécules Max Mousseron (IBMM), UMR 5247-CNRS-UM-ENSCM, Montpellier, France; 2Institut régional du Cancer de Montpellier (ICM), Département de Pharmacie et Pharmacologie, Montpellier, France; 3Department of Analytical Chemistry, Faculty of Pharmacy, Misr University of Science and Technology (MUST), Egypt
P 264Influence of the mixed functionalization of organic ligands in UiO-66-based metal-organic frameworks when used as sorbents in dispersive micro-solid phase extraction
G. González-Rodríguez1, I. Taima-Mancera1, A. Ramos-Lima1, A. Gutiérrez-Serpa1, A.B. Lago3, J. Pasán3, V. Pino1,2
1Departamento de Química, Unidad Departamental de Química Analítica, Universidad de La Laguna, Tenerife, Spain; 2University Institute of Tropical Diseases and Public Health, Universidad de La Laguna, Tenerife, Spain; 3Departamento de Física, Laboratorio de Rayos X y Materiales Moleculares (MATMOL), Universidad de La Laguna, Tenerife, Spain
P 265A pH sensitive-hydrogel in a microextraction procedure for organic compounds in water
R. González-Martín1, I. Pacheco-Fernández1, V. Pino1,2, D. Díaz3,4, J.H. Ayala1, A.M. Afonso1
1Departamento de Química, Unidad Departamental de Química Analítica, Universidad de La Laguna, Tenerife, Spain; 2University Institute of Tropical Diseases and Public Health, Universidad de La Laguna, Tenerife, Spain; 3Instituto de Productos Naturales y Agrobiología del CSIC, Tenerife, Spain; 4Institut für Organische Chemie, Universität Regensburg, Germany
P 266Selective extraction of aliphatic amines by functionalized mesoporous silica-coated solid phase microextraction Arrow
H. Lan, W. Zhang, K. Hartonen, M.L. Riekkola
Department of Chemistry, University of Helsinki, Finland
P 267Hybrid monoliths with metal-organic frameworks in spin columns for extraction of non-steroidal drugs in urine
M. Giesbers, E.J. Carrasco-Correa, E.F. Simó-Alfonso, J.M. Herrero-Martínez
Department of Analytical Chemistry, Faculty of Chemistry, University of Valencia, Spain
P 268BioSPME: an alternative approach to measuring plasma protein binding
N. Corden
LGC, Bioanalysis, Drug Development Solutions, Newmarket Road, Fordham, Ely, UK
P 269A Rapid, Simple, and Efficient Automated Method for the Extraction of Additives from Plastics
B.A. Leffler, C.A. Olsson, C.S. Cashman, B. Liu, A.D. Stell
CEM Corporation, Matthews, USA
P 270Purification of saccharides labeled by cationic tags
D. Moravcova, R. Cmelik, J. Krenkova
Czech Academy of Sciences, Institute of Analytical Chemistry, Brno, Czech Republic
P 271No worries about HPLC analysis of complex matrix-rich samples
A. Piper1, B. Peters2, P. Lewits3, S. Altmaier4
1,2,4Merck KGaA, Instrumental Analytics R&D, Darmstadt, Germany; 3Merck KGaA, Chromatography & Instrumental Analysis, Darmstadt, Germany
P 272Multicationic labels for glycan analysis – synthesis and application
R. Cmelik, J. Krenkova, M. Liskova, F. Foret
Czech Academy of Sciences, Institute of Analytical Chemistry, Brno, Czech Republic
P 273setting up procedure for precise and accurate formation of DBS of uniform area via experimental design and image processing methodology
M. Rmandić1, Y. Dotsikas2, A. Malenović1
1University of Belgrade, Faculty of Pharmacy, Department of Drug Analysis, Belgrade, Serbia; 2Laboratory of Pharmaceutical Analysis, Faculty of Pharmacy, National and Kapodistrian University of Athens, Greece

HPLC for process analytical technology (PAT) and quality assessment in preparative chromatography - Biomolecule purification

P 274THE SIMPLIFICATION OF TWO STEP PROTEIN PURIFICATION BY AUTOMATIZATION FOR MOUSE ANTIBODIES AND 6XHIS-TAGGED GFP
U. Krop, Y. Krauke, K. Monks
KNAUER Wissenschaftliche Geräte GmbH, Berlin, Germany
P 275CHROMATOGRAPHIC BEHAVIOR OF BIVALENT BISPECIFIC ANTIBODIES ON CATION EXCHANGE COLUMNS
L.K. Kimerer1, T. Pabst2, A. Hunter2, G. Carta1
1Department of Chemical Engineering, University of Virginia, Charlottesville, USA; 2AstraZeneca, Gaithersburg, USA
P 276Tuning selectivity of strong cation exchange resins by ligand density variation
M. Schulte, M.T. Stone, R. Skudas, P. Menstell, H. Graalfs
Chromatography R&D, Merck Life Science, Darmstadt, Germany
P 277rapid affinity chromatographic isolation of low-density lipoproteins from human plasma
T. Liangsupree1, E. Multia1, J. Metso2, M. Jauhiainen2, P. Forssén3, T. Fornstedt3, K. Öörni4, A. Podgornik5,6, M.L. Riekkola1
1Department of Chemistry, University of Helsinki, Finland; 2Minerva Foundation Institute for Medical Research and National Institute for Health and Welfare, Helsinki, Finland; 3Department of Engineering and Chemical Sciences, Karlstad University, Sweden; 4Wihuri Research Institute, Atherosclerosis Research Laboratory, Helsinki, Finland; 5Faculty of Chemistry and Chemical Technology, University of Ljubljana, Slovenia; 6Center of Excellence for Biosensors, Instrumentation and Process Control - COBIK, Ajdovščina, Slovenia
P 278Novel isolation of platelet-derived extracellular vesicles from blood plasma by affinity monolithic column, immobilized with anti-human CD61 antibody
E. Multia1, C. Tear1, M. Palviainen2, P. Siljander2, M.L. Riekkola1
1Department of Chemistry, University of Helsinki, Finland; 2EV-group, Molecular and Integrative Biosciences Research Programme, Faculty of Biological and Environmental Sciences, University of Helsinki, Finland; EV-core, University of Helsinki, Helsinki, Finland; Drug Research Program, Faculty of Pharmacy, Division of Pharmaceutical Biosciences, University of Helsinki, Finland
P 279Purification of Phosphorothioated Antisense Oligonucleotides
M. Enmark1,2, J. Bagge1,2, J. Samuelsson1, L. Thunberg3, H. Leek3, F. Limé4, T. Fornstedt1
1Department of Engineering and Chemical Sciences, Karlstad University, Sweden; 2Pharmacognosy, Department of Medicinal Chemistry, Uppsala University, Biomedical Centre, Uppsala, Sweden; 3Early Chemical Development, Pharmaceutical Sciences, R&D BioPharmaceuticals, AstraZeneca, Gothenburg, Sweden; 4Nouryon, Separation Products, Bohus, Sweden
P 280Investigation of impurity profiles for GLP-1 analogue Liraglutide
T. Tran, A. Hermansson, F. Limé
Nouryon/Kromasil, Bohus, Sweden

HPLC for process analytical technology (PAT) and quality assessment in preparative chromatography - CHIRAL separations

P 281ENANTIOMER SEPARATION OF DL-TERT-LEUCINE ON CHIRAL ZWITTERIONIC STATIONARY PHASES BY SUPERCRITICAL FLUID CHROMATOGRAPHY AND HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY
R. Kulágin1, I. Bacskay2, W. Lindner3, A. Felinger1,2,4
1Department of Analytical and Environmental Chemistry, University of Pécs, Hungary; 2MTA-PTE Molecular Interactions in Separation Science Research Group, Pécs, Hungary; 3Department of Analytical Chemistry, University of Vienna, Austria; 4Institute of Bioanalysis, University of Pécs, Medical School, Pécs, Hungary
P 282Chiral high-performance liquid and subcritical fluid chromatography study of some pharmaceutically important ß-carboline, tetrahydroisoquinoline and benzazepine analogs
I. Ilisz1, A. Bajtai1, I. Szatmári2, F. Fülöp2, W. Lindner3, A. Péter1
1Institute of Pharmaceutical Analysis, University of Szeged, Hungary; 2Institute of Pharmaceutical Chemistry, University of Szeged, Hungary; 3Department of Analytical Chemistry, University of Vienna, Austria

HPLC for process analytical technology (PAT) and quality assessment in preparative chromatography - Continuous chromatography

P 283APPLICATION OF SIMULATED MOVING BED (SMB) CHROMATOGRAPHY FOR CONTINUOUS PURIFICATION OF SMALL AND LARGE MOLECULES
Y. Krauke, C. Benkhäuser, M. Lübbert, K. Monks
KNAUER Wissenschaftliche Geräte GmbH, Berlin, Germany
P 284CONTINUOUS CHROMATOGRAPHIC SEPARATION FOR MANNOSE PRODUCTION FROM GUAR GUM USING PILOT PLANT OF SMB TYPE
S. Gillarova, S. Henke, T. Svoboda, Z. Bubnik, M. Slukova
Department of Carbohydrates and Cereals, FFBT, University of Chemistry and Technology, Prague, Czech Republic

HPLC for process analytical technology (PAT) and quality assessment in preparative chromatography - Fundamentals

P 285Ultra-high-pressure ion chromatography with suppressed conductivity detection at 70 MPa using columns packed with 2.5 m anion-exchange particles
S. Wouters1, J.L. Dores-Sousa1, Y. Liu2, C.A. Pohl2, S. Eeltink1
1Vrije Universiteit Brussel (VUB), Department of Chemical Engineering, Brussels, Belgium; 2Thermo Fisher Scientific, Sunnyvale, USA

HPLC for process analytical technology (PAT) and quality assessment in preparative chromatography - Other

P 286MINIATURE, MODULAR CAPILLARY LIQUID CHROMATOGRAPHY SYSTEM FOR PHARMACEUTICAL INDUSTRY
L.J. Coates1,2, A.R. Gooley1,2, P.R. Haddad1, B. Paull1, H.J. Wirth*1,2
1ARC Centre for Portable Analytical Technologies (ASTech), University of Tasmania, Australia; 2Trajan Scientific and Medical, Ringwood, Australia
P 287A versatile semi-preparative HPLC platform, including HR-MS and NMR, to support process development in industrial biotechnology
B. Ozalp, L. Duchateau, P. Lankhorst, S. Gosiewska

DSM Biotechnology Center, Delft, The Netherlands
P 288Evaluation of nucleic acids adsorption utilizing size-exclusion chromatography
S.I. Fujii, M. Yoshioka
National Metrology Institute of Japan (NMIJ), National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan

HPLC for process analytical technology (PAT) and quality assessment in preparative chromatography - Processes

P 289Flow Injection Analysis with MS detection as PAT tool in chemical synthesis of therapeutic oligonucleotides
P. Stasica, B. Andrews
GlaxoSmithKline, Analytical Tech & Automation, Stevenage, UK

LC/MS and hyphenation - Biomedical LC-MS/precision medicine

P 290A quantitative screening method for remifentanil in brain and whole blood to evaluate the predictive performance of target controlled infusion systems
L. Van Hese1,3, T. Theys2, S. De Vleeschouwer2, S. Rex1, E. Cuypers3
1Department of Anaesthesiology, University Hospitals Leuven; Department of Cardiovascular Sciences, KU Leuven, Belgium; 2Neurosurgery Department, University Hospitals Leuven; Laboratory for Experimental Neurosurgery and Neuroanatomy, Department of Neurosciences, Leuven, Belgium; 3Toxicology and Pharmacology Department, KU Leuven, Belgium
P 291Mobile phase optimization applying a design of experiments approach to increase the signal response in LC-MS/MS of bradykinin and its internal standard
T. Gangnus, B.B. Burckhardt
Heinrich Heine University Dusseldorf, Institute of Clinical Pharmacy and Pharmacotherapy, Dusseldorf, Germany

LC/MS and hyphenation - Biopharmaceutical LC-MS

P 292AN EFFICIENT METHOD FOR THE DETERMINATION OF TRACE EXCIPIENT IMPURITIES IN BIOTHERAPEUTIC DRUG PRODUCTS CONTAINING POLYSORBATE
R. Birdsall, B. Koshel, Y.Q. Yu
Waters Corp Milford, MA, USA
P 293IMPROVING ONLINE ION EXCHANGE CHROMATOGRAPHY-MASS SPECTROMETRY FOR DOMAIN SPECIFIC CHARGE VARIANT ANALYSIS OF MONOCLONAL ANTIBODIES
M.A. Lauber1, Q. Wang1, Y. Leblanc2, G. Chevreux2, S. Ippoliti1, Y.Q. Yu1
1Waters Corporation, Milford, USA; 2LFB Biotechnologies, Courtabœuf, Les Ulis, France
P 294LC-MS/MS ASSESSMENT OF MONOCLONAL ANTIBODY STABILITY: MONITORING TRYPTOPHAN AND METHIONINE OXIDATION
K. Duraisamy, T. Gandhi, M. Raina, R. Krovidi
Syngene International Ltd, Bangalore, India
P 295A UHPLC-MS PLATFORM FOR STABILITY-INDICATING OLIGONUCLEOTIDE ANALYSIS: ASSESSMENT WITH A 13-MER OLIGONUCLEOTIDE N3’-P5’ THIO-PHOSPHORAMIDATE
P. Yogendrarajah, W. Verluyten, J.P. Boon, A. Van Cleempoel, M. Hellings
Small Molecule Pharmaceutical Development/Analytical Development, Discovery, Product Development and Supply, Janssen Pharmaceutical Companies of Johnson & Johnson, Beerse, Belgium
P 296DEVELOPMENT OF AN HPLC-MS BASED APPROACH FOR HYDROLYSIS PRODUCTS OF TRITERPENOID SAPONINS DETERMINATION IN THE PANAX SPECIES
I. Ananyeva, M. Abashev, E. Stekolshchikova, I. Rodin, A. Stavrianidi
Lomonosov Moscow State University, Faculty of Chemistry, Moscow, Russia
P 297DETERMINATION OF THORIUM-CONJUGATED MONOCLONAL ANTIBODY BY HYBRID-LC-MS/MS FROM MONKEY PLASMA
T. Hetzel1, D. Bochmann1, C. Rolke1, E. Busemann2, M.J.Gnoth1
1Bayer AG, Wuppertal, Germany; 2Bayer AG, Berlin, Germany
P 298TANDEM UHPLC OPERATION FOR HIGH-THROUGHPUT LC-MS PEPTIDE MAPPING ANALYSES
M. Samonig, S. Patzelt, C. Paul, G. Greco, M. Ruehl, R. Swart
Thermo Fisher Scientific, Germering, Germany
P 299Simultaneous quantification of human insulin and three insulin analogues in plasma using protein precipitation LC-MS/MS
O.A. Istrate1, N.J. Nielsen1, T. Søeborg2
1Affiliat. Department of Plant and Environmental Sciences, University of Copenhagen, Denmark; 2Affiliat. Department of Development LC-MS Bioanalysis, Novo Nordisk A/S, Måløv, Denmark
P 300Eliminate trifluoroacetic acid from LC-MS analysis of biopharmaceuticals
M. Khalikova, F. Švec, L. Nováková, J. Lenčo
Department of Analytical Chemistry, Faculty of Pharmacy in Hradec Kralove, Charles University, Hradec Kralove, Czech Republic
P 301Fast LC-MS/MS analysis of Vitamin D metabolites on newly developed micro-flow ESI ion source with cartridge column
T. Bieńkowski1, R. Rola2, M. Szumski1,2, I. Tomaszewska1, K. Kowalski1, P. Kalicki1, M. Książkiewicz1, S. Studzińska2
1MS Ekspert sp. z o.o. Gdańsk, Poland; 2Chair of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Toruń, Poland
P 302Robust Extraction, Separation and Quantitation of Structural Isomer Steroids From Human Plasma by SPE with LC-MS/MS Detection
J. Bardsley
Thermo Fisher Scientific, Runcorn, United Kingdom
P 303Improving LC-MS Separations of Peptides with Difluoroacetic Acid Ion Pairing
J.M. Nguyen1,2, X. Liu2, M.A. Lauber2
1Faculty of Science, University of Copenhagen, Denmark; 2Waters Corporation, Milford, United States
P 304Validation of a method for the determination of novel selenium containing antimetastatic drug candidate PA-27 in mouse plasma by UPLC-MS-MS
V. Krizanovska1,2, S. Grinberga1, E. Sevostjanovs1, P. Arsenyan1
1Latvian Institute of Organic Synthesis, Riga, Latvia; 2Faculty of Materials Science and Applied Chemistry, Riga Technical University, Latvia
P 305Reproducible chromatographic performance enables low ppm LC-MS detection of Host Cell Proteins (HCPs) in biotherapeutics
A.J. Claydon, P. Widdowson, A. Williamson
Thermo Fisher Scientific, Runcorn, UK; Thermo Fisher Scientific, Hemel Hempstead, UK
P 306Oligonucleotide analysis using a mixed mode stationary phase on a 2D-LC system hyphenated to mass spectrometry
J. Haglöf, J. Wu Ludvigsson
Medicinal Chemistry, Uppsala University, Sweden; PT&D, Astra Zeneca, Gothenburg, Sweden
P 307Automated proteolytic digestion for high precision peptide mapping and in-depth characterization of biotherapeutic proteins
A. Schwahn1, S. Millán-Martín2, S. Carillo2, J. Bones2, D.B. Kristensen3, K. Cook1
1Thermo Fisher Scientific, Reinach, Switzerland, Hemel Hempstead, UK; 2Characterisation and Comparability Laboratory, NIBRT – National Institute for Bioprocessing Research and Training, Dublin, Ireland; 3Symphogen A/S, Copenhagen, Denmark
P 308Novel way to Introduce the traditional salt based chromatography technique of ion exchange charged variant analysis of biopharmaceutical proteins into high resolution MS – CVA/MS a new multi-attribute method
A. Schwahn1, F. Füssl2, J. Bones2, K. Scheffler1, K. Cook1
1Thermo Fisher Scientific, Reinach, Switzerland, Germering, Germany, Hemel Hempstead, UK; 2Characterisation and Comparability Laboratory, NIBRT – National Institute for Bioprocessing Research and Training, Dublin, Ireland

LC/MS and hyphenation - High-resolution MS

P 309COUPLING SIZE EXCLUSION CHROMATOGRAPHY TO HIGH RESOLUTION MASS SPECTROMETRY FOR THE CHARACTERIZATION OF POLYMERS
M. Dunkle1, A. Konig1, J. Stutzman2, G. Groeneveld3, E. Mes1, P. Schoenmakers3
1Dow Benelux, Analytical Science, Terneuzen, The Netherlands; 2Dow Chemical, Analytical Science, Midland, Michigan, USA; 3University of Amsterdam, Van 't Hoff Institute for Molecular Sciences, Amsterdam, The Netherlands
P 310SCREENING OF ENVIRONMENTAL CONTAMINANTS IN HUMAN PLASMA WITH UHPLC/Q-TOF MS
Z.Y. Chen, C.Y. Chen
Institute of Environmental Health, National Taiwan University, Taipei City, Taiwan
P 311SPLITTING AND MEASURING A LIQUID CHROMATOGRAPHIC FLOW – HOW HARD CAN IT BE?
P. Denti, L.M.V. Malmquist, G. Tomasi, J.H. Christensen
Department of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg, Denmark
P 312High resolution chemical analysis and machine learning for the identification of pharmaceutical, pesticide and illicit drug compounds in water
H. Rapp-Wright1,2, F. Regan1, B. White1, L. Barron2
1DCU Water Institute and School of Chemical Sciences, Dublin City University, Glasnevin, Dublin, Ireland; 2Analytical & Environmental Sciences Division, King’s College London, Franklin-Wilkins Building, London, UK
P 313Peptide analysis: the HPLC force awakens
F. Dal Bello1, M. Zorzi1, P. Giacobini2,3, C. Medana1
1Department of Molecular Biotechnology and Health Science, University of Torino, Italy; 2Jean-Pierre Aubert Research Center, Laboratory of Development and Plasticity of the Neuroendocrine Brain, Lille, France; 3University of Lille, FHU 1000 Days for Health, France

LC/MS and hyphenation - HP-TLC-MS

P 314OPEN SOURCE 3D PRINTED ADD-ON FOR AUTOMATED ELUTION HEAD-BASED HPTLC-MS
T. Häbe, D. Fichou, G.E. Morlock
Chair of Food Sciences, Justus Liebig University Giessen, Germany
P 315Detection and identification of antibacterial compounds present in Maqui leaves (Aristotelia chilensis) by HPTLC-bioassay-MS
J. Avendaño1, P. Aqueveque1, M. Aranda2, K. Henríquez-Aedo1,2
1Laboratory of Biotechnology and Genetics of Food. Department of Food Science and Technology, Faculty of Pharmacy, University of Concepcion, Barrio Universitario s/n, Concepcion, Chile; 2Laboratory of Advanced Research on Foods and Drugs. Department of Food Science and Technology, Faculty of Pharmacy, University of Concepcion, Barrio Universitario s/n, Concepcion, Chile

LC/MS and hyphenation - LC-MS data analysis

P 316Structure elucidation of di- and trisaccharides by liquid chromatography coupled to tandem mass spectrometry
A. Martín-Ortiz1, R. Lebrón-Aguilar2, A.I. Ruiz-Matute1, F.J. Moreno3, M.L. Sanz1
1Instituto de Química Orgánica General (CSIC) Madrid, Spain; 2Instituto de Química-Física “Rocasolano” (CSIC) Serrano, Madrid, Spain; 3Instituto de Investigación en Ciencias de la Alimentación (CSIC-UAM) Nicolás Cabrera, Madrid, Spain
P 317Effective identification and management of degradant data in process development
P. Russell, A. Anderson, S.K. Bhal, J. DiMartino
Advanced Chemistry Development (ACD/Labs), Toronto, Canada,
P 318Determination of doxycycline in claws of chickens by simple and fast
UHPLC -MS/MS method

I. Szymanek-Bany, A. Gajda, E. Nowacka-Kozak, M. Gbylik-Sikorska, A. Posyniak
Department of Pharmacology and Toxicology, National Veterinary Research Institute, Pulawy, Poland
P 319An UHPLC/MS/MS method for the determination of aminoglycoside antibiotics in a variety of matrices of animal origin
E. Nowacka-Kozak, A. Gajda, M. Gbylik-Sikorska, A. Posyniak
National Veterinaty Research Institute in Pulawy, Department of Pharmacology ang Toxicology
P 320An integrated software platform for untargeted LC-MS metabolomics
G. Poloniato1, R. Arduni2, M. Stocchero1, E. Baraldi1, G. Giordano1
1Laboratory of Mass Spectrometry and Metabolomics, Department of Women’s and Children’s Health, University of Padova, Italy; 2S-IN Soluzioni Informatiche, Vicenza, Italy
P 321An updated software solution for rational, QbD-aligned LC-MS method development
A. Vazhentsev, M.J. Binnington, K.K. Bhagchandani
Advanced Chemistry Development (ACD/Labs), Toronto, Canada
P 322Development of peak-tracking algorithms for use in data analysis and method optimization in multi-dimensional liquid chromatography
S.R.A. Molenaar1, D.R. Stoll2, R.A.H. Peters1,3, G.W. Somsen4, P.J. Schoenmakers1, B.W.J. Pirok1
1University of Amsterdam, van 't Hoff Institute for Molecular Sciences, Analytical-Chemistry Group, Amsterdam, The Netherlands; 2Department of Chemistry, Gustavus Adolphus College, Saint Peter, Minnesota, United States; 3DSM Coating Resins, Waalwijk, The Netherlands; 4BioAnalytical Chemistry, Amsterdam Institute for Molecules, Medicines and Systems, Vrije Universiteit Amsterdam, The Netherlands
P 323Fast and selective LC/DAD/MS method to determine phenolic compounds from Arrayan Fruit (Luma apiculata) after ultrasound-assisted extraction
J. Carrasco-Sandoval1, D. Peterssen-Fonseca1, M. Aranda1, K. Henríquez-Aedo1,2*
1Laboratory of Advanced Research on Foods and Drugs. Department of Food Science and Technology, Faculty of Pharmacy, University of Concepcion, Barrio Universitario s/n, Concepcion, Chile; 2Laboratory of Biotechnology and Genetics of Food. Department of Food Science and Technology, Faculty of Pharmacy, University of Concepcion, Barrio Universitario s/n, Concepcion, Chile

LC/MS and hyphenation - LC-MS of Small Molecules

P 324LC AND GC INTEGRATED IN ONE MS SYSTEM WITH COLD ELECTRON IONIZATION ION SOURCE
A.B. Fialkov1, S. Tsizin1, A. Amirav2
1School of Chemistry, Tel Aviv University, Israel; 2Tel Aviv University, Israel and Aviv Analytical LTD, Tel Aviv, Israel
P 325LC-MS HYPHENATION TECHNIQUES IN THE FIELD OF LITHIUM ION BATTERY ELECTROLYTES –
STRUCTURE ELUCIDATION OF PHOSPHORUS DECOMPOSITION PRODUCTS

J. Henschel, K. Kösters, M. Winter, S. Nowak
University of Münster, MEET Battery Research Center, Institute of Physical Chemistry, Münster, Germany
Helmholtz Institute Münster (HI MS), IEK-12, Forschungszentrum Jülich GmbH, Münster, Germany
P 326ANALYTICAL QUALITY BY DESIGN APPROACH IN UPLC-MS METHOD DEVELOPMENT FOR THE ANALYSIS OF BUDESONIDE, FORMOTEROL AND THEIR RELATED COMPOUNDS
F.L. Alkhateeb, P. Rainville
Waters Corporations
P 327PHARMACEUTICAL COUNTERION ANALYSIS USING HYDROPHILIC INTERACTION CHROMATOGRAPHY AND ITS APPLICATION
T. Takeuchi
Teijin Institute for Bio-medical Research, Teijin Pharma Limited., Tokyo, Japan
P 328CARRYOVER MITIGATION USING NEEDLE WASH SOLVENT CHEMISTRY AND AUTOSAMPLER FEATURES OF A UPLC-MS SYSTEM
C. DesJardins, Z. Li, P. McConville
Waters Corporation
P 329Determination of impurities reducing UV transmittance of ethylene glycol by ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry
L. Li*, Z. Peng, Y. Zhang, C. Wang
Sinopec Shanghai Research Institute of Petrochemical Technology, Shanghai, China
P 330New insight in urinary peptidomics: innovative strategy for short peptide analysis
A. Cerrato1, C.M. Montone1, C. Cavaliere1, S. Piovesana1, A.L. Capriotti1, A Laganà1,2
1Chemistry Department, University La Sapienza, Rome, Italy; 2CNR NANOTEC, Campus Ecotekne of the University of Salento, Lecce, Italy
P 331Sensitive and robust LC–MS/MS analysis of digoxin in human plasma through optimization of in-source adduct formation
S. Keane, G. Wallace, C. Munday, M. Wright
LGC, DDS, Fordham, Ely, Great Britain
P 332Analysis of polar metabolites? HILIC-HRMS, a substitute for ion-pair LCMS
W. Coppes, R. Ramaker, S. Pous Torres, R. Maleki-Seifar, L. Coulier
DSM Biotechnology Center, DSM Food Specialties BV, Delft, The Netherlands
P 333Development of HPLC-MS methods for monitoring of organic dyes degraded through VPHP exposure
K. Syslova, A. Zolal, L. Filip, M. Mikoska
Department of Organic Technology, University of Chemistry and Technology Prague, Czech Republic
P 334Small-molecule pattern of chronic stress
M. Mikoska1, K. Syslova1, O. Petrak2
1Department of Organic Technology, University of Chemistry and Technology Prague, Czech Republic; 23rd Department of Medicine, Department of Endocrinology and Metabolism, General University Hospital, Prague, Czech Republic
P 335Scouting, purification, and re-analysis on a combined analytical and preparative LC/MS system
I. Spuling, F. Rieck
Agilent Technologies, Waldbronn, Germany
P 336OPTIMIZATION OF NEGATIVE ION RESPONSE IN ELECTROSPRAY IONIZATION
H. KOČOVÁ VLČKOVÁ1, I. LHOTSKÁ1, P. MLADĚNKA2, P. SOLICH1, L. NOVÁKOVÁ1
1Department of Analytical Chemistry, Faculty of Pharmacy in Hradec Králové, Charles University, Czech Republic; 2Department of Pharmacology and Toxicology, Faculty of Pharmacy in Hradec Králové, Charles University, Czech Republic
P 337Alkaloid profiling of food tannins using high resolution mass spectrometry
T. Nardin1*, P. Apostoli1, R. Larcher1, F. Abballe2
1Technology Transfer Centre, Fondazione Edmund Mach, San Michele all'Adige, Italy; 2Thermofisher Scientific, Italy

LC/MS and hyphenation - Microscale LC-MS

P 338HIGH-SENSITIVITY QUANTITATION OF HEPARAN SULFATE DISACCHARIDES USING SALT GRADIENTS IN HILIC-WAX CHROMATOGRAPHY
G. Tóth1,2, K. Vékey1, L. Drahos1, V. Horváth2,3, L. Turiák1
1MS Proteomics Research Group, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Budapest, Hungary; 2Department of Inorganic and Analytical Chemistry, Budapest University of Technology and Economics Budapest, Hungary; 3MTA-BME Computation Driven Chemistry Research Group, Budapest, Hungary
P 339High-Sensitivity Analysis of drugs in ultra-small volumes plasma samples using Micro-Flow LC-MS/MS
D. Vecchietti1, M. Levi2, H. Terada1, J. EDWARDSEN3, S. IMAMURA1, K. WATANABE1
1SHIMADZU Corporation, LC Business Unit, Kyoto, Japan; 2SHIMADZU Corporation, MS Business Unit, Kyoto, Japan; 3SHIMADZU Scientific Instruments, Columbia MD, US

LC/MS and hyphenation - Multidimensional chromatography and MS coupling

P 340COMPARISON OF COMPLEX PETROLEUM SAMPLES BY OFF-LINE COMPREHENSIVE TWO-DIMENSIONAL CHROMATOGRAPHY SECXRPLC COUPLED WITH SPECIFIC ICP-MS/MS DETECTION
M. Bernardin1,2, F. Bessueille-Barbier1, A. Le Masle2, C.P. Lienemann2, S. Heinisch1
1Université de Lyon, Institut des Sciences Analytiques, CNRS, ENS Lyon, Villeurbanne, France; 2IFP Energies nouvelles, Rond-point de l’échangeur de Solaize, France
P 3412D LC HRMS ANALYSIS OF BIVALIRUDIN AND ITS DEGRADATION PRODUCTS
A. Vukoja1, S. Galović1, N. Galić2
1PLIVA Croatia Ltd., Analytics R&D, Zagreb, Croatia; 2Department of Chemistry, Faculty of Science, University of Zagreb, Croatia
P 3421D/2D-Multi-Valve-UPLC/MS/MS-system

M. Schiell
Sanofi Frankfurt Germany
P 343COMPREHENSIVE TWO-DIMENSIONAL LIQUID CHROMATOGRAPHY COUPLED TO MASS SPECTROMETRY FOR THE DETERMINATION OF THE POLYPHENOLIC PROFILE IN THREE CULTIVARS OF BRASSICA JUNCEA
K. Arena1, F. Cacciola2, P. Dugo1,3,4, L. Mondello1,3,4
1Dipartimento di Scienze Chimiche, Biologiche, Farmaceutiche ed Ambientali, University of Messina, Italy; 2Dipartimento di Scienze Biomediche, Odontoiatriche e delle Immagini Morfologiche e Funzionali, University of Messina, Italy; 3Chromaleont s.r.l., c/o Dipartimento di Scienze Chimiche, Biologiche, Farmaceutiche ed Ambientali, University of Messina, Italy; 4Department of Medicine, University Campus Bio-Medico of Rome, Italy
P 344DETERMINATION OF THE POLYPHENOLIC FRACTION OF PISTACIA VERA L.KERNEL EXTRACTS BY COMPREHENSIVE TWO-DIMENSIONAL LIQUID CHROMATOGRAPHY COUPLED TO MASS SPECTROMETRY DETECTION
D. Mangraviti1, K. Arena1, F. Cacciola2, M. Zoccali1, F. Rigano3, P. Dugo1,3,4, L. Mondello1,3,4
1Dipartimento di Scienze Chimiche, Biologiche, Farmaceutiche ed Ambientali University of Messina, Italy; 2Dipartimento di Scienze Biomediche, Odontoiatriche e delle Immagini Morfologiche e Funzionali University of Messina, Italy; 3Chromaleont s.r.l., c/o Dipartimento di Scienze Chimiche, Biologiche, Farmaceutiche ed Ambientali, University of Messina, Italy; 4Department of Medicine, University Campus Bio-Medico of Rome, Italy
P 345LOW-FLOW ON-LINE 2D LCMS HYPHENATED WITH HRAM MS FOR COMPREHENSIVE PROTEOME PROFILING
O. Boychenko, C. Pynn, W. Decrop, P. Jehle, M. De Pra
Thermo Fisher Scientific, Germering, Germany
P 346CHARACTERIZATION OF ASTAXANTHIN-ENRICHED EXTRACTS FROM HAEMATOCOCCUS PLUVIALIS BY COMPREHENSIVE TWO-DIMENSIONAL LIQUID CHROMATOGRAPHY COUPLED TO MASS SPECTROMETRY DETECTION
R. Gallego1, K. Arena2, F. Cacciola3, L. Mondello2,4,5, E. Ibáñez1, M. Herrero*1
1Laboratory of Foodomics, Institute of Food Science Research (CIAL, CSIC), Madrid, Spain; 2Dipartimento di Scienze Chimiche, Biologiche, Farmaceutiche e Ambientali, University of Messina, Italy; 3Dipartimento di Scienze Biomediche, Odontoiatriche e delle Immagini Morfologiche e Funzionali, University of Messina, Italy; 4Chromaleont s.r.l., c/o Dipartimento di Scienze Chimiche, Biologiche, Farmaceutiche ed Ambientali, University of Messina, Italy; 5Unit of Food Science and Nutrition, Department of Medicine, University Campus Bio-Medico of Rome, Italy
P 347DECIPHERING THE COMPLEX DISTRIBUTIONS OF CELLULOSE-ETHERS
T.S. Bos1, J. Purmova2, L. Karlson2, R. Haselberg1, P.J. Schoenmakers3, G.W. Somsen1
1BioAnalytical Chemistry, Amsterdam Institute for Molecules, Medicines and Systems, Vrije Universiteit Amsterdam, The Netherlands; 2Nouryon, Deventer, The Netherlands; 3Van 't Hoff Institute for Molecular Science (HIMS), University of Amsterdam, The Netherlands
P 348Comparison of online comprehensive RP × RP vs HILIC × RP with enhanced trapping modulation coupled to High resolution Mass Spectrometry for microalgae peptidomics: a critical evaluation
E. Salviati1,2, E. Sommella3, G. Pepe1, E. Novellino3, P. Campiglia1
1Department of Pharmacy, University of Salerno, Fisciano, Italy; 2Program in Drug Discovery and Development, University of Salerno, Fisciano, Italy; 3Department of Pharmacy, University of Naples Federico II, Italy
P 349Mixed Mode Stationary Phases and their Benefit in LC x LC
S. Bäurer, S. Neubauer, M. Lämmerhofer
Pharmaceutical (Bio-)Analysis, Institute of Pharmaceutical Sciences, University of Tübingen, Germany
P 350Intact Analysis of Bio-pharmaceuticals by Hydrophobic Interaction/Reversed Phase 2D-LC/MS System
Y. Li1, S. Kondaveeti2, G. Staples3, A. Coffey4
1Agilent Technologies, Newport, USA; 2Agilent Technologies, Wilmington, USA; 3Agilent Technologies, Santa Clara, USA; 4Agilent Technologies, Church Stretton, UK
P 351Design and application of a scalable automated platform using multi-dimensional chromatography for impurity characterization of an RNAi therapeutic
G. Jones
Analytical Sciences, Alnylam Pharmaceuticals, Cambridge, USA
P 352A flexible custom-made multi-dimensional LC-MS system and automated control software with one graphical user interface for next level online characterization of biotherapeutics
K. Mayr1, A. Gärtner1, X. Reiser1, T. Maetzke2, T. Nachtigall3, M. Förster3, A. Vogt1, M. Mølhøj1
1Roche Pharma Research and Early Development (pRED), Large Molecule Research, Roche Innovation Center Munich, Penzberg, Germany; 2HPLConsult GmbH, Basel, Switzerland; 3Thermo Fisher Scientific GmbH, Dreieich, Germany

LC/MS and hyphenation - Other

P 353OPTIMIZATION OF SELECTED PARAMETERS FOR PHOSPHATIDYLETHANOL (PETH 16:0/18:1) DETECTION BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY-TANDEM MASS SPECTROMETRY
M. Machková1,3, E. Gonová3, J. Kvetková2, M. Knapková1, S. Hrouzková3
1Newborn Screening Center of Slovak Republic, Children’s University Hospital of Banská Bystrica, Námestie L. Svobodu Banská Bystrica, Slovak republic; 2Specialized psychiatric institut for treatment of addiction Predna Hora, Muránska Huta, Muráň, Slovak republic; 3Slovak University of Technology in Bratislava, Faculty of Chemical and Food Technology, Institute of Analytical Chemistry,Bratislava, Slovak republic
P 354detecting stamps by sers and maldi-ms
P. Breuer1, F. Ariese2, G. Somsen3, P. Schoenmakers1
1University of Amsterdam, Faculty of Science, Van ‘t Hoff Institute for Molecular Sciences, Amsterdam, The Netherlands; 2Vrije Universiteit, Faculty of Science, Biophotonics and Medical Imaging, Amsterdam, The Netherlands; 3Vrije Universiteit, Faculty of Science, Biomolecular Analysis and Spectroscopy, Amsterdam, The Netherlands
P 355Evolution of LC-NMR: hyphenation at 1.5 Tesla
P. Sabatino1, M. van Engelen1, M. Pursch2, H. Eghbali1, A. König1, R. Zeigler3, K. Meyer4, J. Kolz4, A. Schweizer-Theobaldt1
1Corporate R&D, Dow Benelux B.V., Terneuzen, The Netherlands; 2Corporate R&D, Dow Stade Produkt. GmbH&Co Stade, Germany; 3Corporate R&D, The Dow Chemical Company, Freeport, TX, U.S.A.; 4Magritek GmbH, Aachen, Germany
P 356Inorganic speciation of bio-oils and their feedstocks with GPC ICP MS / ESI MS & SFC ESI MS/ ICP MS
V. Garcia-Montoto1, P. Denti2, L. Malmquist2, G. Tomasi2, S. Verdier3, J.H. Christensen2, B. Bouyssiere1
1Energy and Environment Solutions E2S / University of Pau & Pays Adour, Institut des Sciences Analytiques et de Physico-chimie pour l'Environnement et les Matériaux (IPREM), Pau, France; 2Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Denmark; 3Haldor Topsoe A/S, Kgs. Lyngby, Denmark
P 357Improving universal quantitative response in polymer LC
W. Knol1, B. Pirok1, R. Peters1,2, P. Schoenmakers1
1van ’t Hoff Institute for Molecular Sciences, Analytical Chemistry Group, University of Amsterdam, The Netherlands; 2DSM Coating Resins, Waalwijk, The Netherlands

LC/MS and hyphenation - Quantitation in LC/MS

P 358HILIC ESI–MS/MS ANALYSIS OF MODIFIED OLIGONUCLEOTIDES AND THEIR SYNTHETIC METABOLITES IN SERUM SAMPLES
A. Kaczmarkiewicz, Ł. Nuckowski, S. Studzińska
Nicolaus Copernicus University in Toruń, Poland
P 359IDENTIFICATION OF POLYPHENOLS IN BRANDIES AND WINES BY ULTRAHIGH PERFORMANCE LIQUID CHROMATOGRAPHY AND QUADRUPOLE-ORBITRAP MASS SPECTROMETRY
M. Alimzhanova, B. Kenessov
Al-Farabi Kazakh National University
P 360A LIQUID CHROMATOGRAPHY MASS SPECTROMETRY APPROACH FOR PURITY DETERMINATION OF PEPTIDES
G. Drinkwater, D. Ruperez Cebolla, K. Groves, M. Quaglia
LGC, Teddington, Middlesex, TWII 0LY
P 361QUANTIFICATION OF NEW CHEMICAL ENTITIES AT LOW CONCENTRATION IN EXTRACELLULAR SOLUTIONS USED IN A HERG ASSAY
W. Briône, E. Schenkel, J. Pollicand, L. Lilti, M. Van Hulle, L. Dinaux, F. Delaruelle
UCB. Product Development, Braine L'Alleud, Belgium
P 362LC-MS HYPHENATION TECHNIQUES IN THE FIELD OF LITHIUM ION BATTERY ELECTROLYTES –QUANTIFICATION OF ORGANO(FLUORO)PHOSPHATES
K. Kösters1, J. Henschel2, M. Winter3, S. Nowak4
1University of Münster, MEET Battery Research Center, Institute of Physical Chemistry, Münster, Germany; 2University of Münster, MEET Battery Research Center, Institute of Physical Chemistry, Münster, Germany; 3University of Münster, MEET Battery Research Center, Institute of Physical Chemistry, Münster, Germany; 2Helmholtz Institute Münster (HI MS), IEK-12, Forschungszentrum Jülich GmbH, Münster, Germany; 4,1University of Münster, MEET Battery Research Center, Institute of Physical Chemistry, Münster, Germany
P 363CONFIRMATORY TESTING OF 70 DRUGS IN HUMAN URINE BY LC/MS/MS FOR PAIN MANAGEMENT
W.H. Campbell, S. Hassan
Phase Analytical Technology, LLC
P 364IC-MS/MS FOR THE ROUTINELY IDENTIFICATION AND DETERMINATION OF COMMON AND EMERGING DISINFECTION BY-PRODUCTS IN DRINKING WATERS
M. Fungi, M. Bocina, R. Binetti, M.C. Bruzzoniti, M. Castiglioni, L. Meucci, M. Politi, L. Rivoira
Department of Chemistry, University of Turin, Italy; SMAT Research Center, Società Metropolitana Acque Torino S.p.A., Italy
P 365GROWING MS ADOPTION: A “SELF-DRIVING” MASS SPECTROMETER DESIGNED FOR NON-MS EXPERTS
K. Covert, M. Ostrowski
Agilent Technologies, Santa Clara, USA
P 366Identification and Quantification of Histamine and its Main Metabolites in Urine Samples from IBS Patients and Healthy Volunteers by use of UPLC-MS/MS
M. Nelis1, A. Kensert1, L. Decraecker2, G. Boeckxstaens2, P. Augustijns1, D. Cabooter1
1University of Leuven, Department of Pharmaceutical & Pharmacological Sciences, Leuven, Belgium; 2University of Leuven, Department of Clinical and Experimental Medicine, Leuven, Belgium
P 367A comparative study of UniSpray and Electrospray for the ionization of neuropeptides in LC-MS/MS
J. Bongaerts1,2, Y. Van Wanseele1, L. Van Oudenhove3, K. Segers1,2, M. Van Hulle3, D. De Bundel1, I. Smolders1, D. Mangelings2, Y. Vander Heyden2, A. Van Eeckhaut1
1Dept. of Pharmaceutical Chemistry, Drug Analysis and Drug Information, Center for Neurosciences (C4N), Vrije Universiteit Brussel, Belgium; 2Dept. of Analytical Chemistry, Applied Chemometrics and Molecular Modelling, Vrije Universiteit Brussel, Belgium; 3Waters Belgium, Zellik, Belgium
P 368HILIC-UHPLC/MS for quantitative lipidomics: An optimized and validated method for the analysis of body fluids
M. Chocholoušková1, D. Wolrab1, R. Jirásko1, D. Vrána2, B. Melichar2, M. Holčapek1
1Department of Analytical Chemistry, University of Pardubice, Czech Republic; 2Faculty of Medicine and Dentistry, Department of Urology, Palacký University, Olomouc, Czech Republic
P 369Quantification of Salivary Cortisone and Cortisol without Derivatization by QTRAP micro LC-MS/MS Using a Surrogate Calibrant Approach
E. Aydin1, B. Drotleff1, H. Noack2, B. Derntl2, M. Lämmerhofer1
1University of Tübingen, Institute of Pharmaceutical Sciences, Pharmaceutical (Bio-)Analysis, Tübingen, Germany; 2University Hospital Tübingen, Clinic for Psychiatry and Psychotherapy, Innovative Neuroimaging, Tübingen, Germany
P 370Improved Separation of Vitamin D Metabolites and Their Epimers
B. Maule, C. Frantz, C. Muraco, M. Ye
MilliporeSigma, Bellefonte, USA
P 371AQbD-oriented MS and UPLC method development for glycopeptide antibacterials assessment in suitable pharmaceutical forms
A. Stajić1, G. Forsdahl2, B. Jančić-Stojanović1, M. Medenica3
1Department of Drug Analysis, University of Belgrade – Faculty of Pharmacy, Belgrade, Serbia; 2Department of Pharmacy, University of Tromsø, Norway; 3Department of Physical Chemistry and Instrumental Methods, University of Belgrade – Faculty of Pharmacy, Belgrade, Serbia
P 372Determination of selected testosterone esters in blood serum of slaughtered animals by liquid chromatography with tandem mass spectrometry
I. Matraszek-Zuchowska, B. Wozniak, K. Sielska, A. Posyniak
Department of Pharmacology and Toxicology, National Veterinary Research Institute-State Research Institute, Pulawy, Poland
P 373Determination of stilbenes and resorcylic acid lactones in animals’ urine using QuEChERS application and liquid chromatography tandem mass spectrometry detection
I. Matraszek-Zuchowska, B. Wozniak, A. Klopot, A. Posyniak
Department of Pharmacology and Toxicology, National Veterinary Research Institute-State Research Institute, Pulawy, Poland
P 374Determination of steroid hormones in the urine of slaugter animals based on QuEChERS extraction and liquid chromatography - tandem mass spectrometry
B. Wozniak, I. Matraszek-Zuchowska, A. Klopot, A. Posyniak
Department of Pharmacology and Toxicology, National Veterinary Research Institute, Pulawy, Poland
P 375Evaluation of the matrix effect in LC-MS methods for determination of steroid hormones in biological samples of animal origin: A comparison of three approaches
B. Wozniak, I. Matraszek-Zuchowska
Department of Pharmacology and Toxicology, National Veterinary Research Institute, Pulawy, Poland
P 376A quality-controlled and design of experiments-based approach for the optimization of the chromatographic solvent composition for the determination of Substance P and Hemokinin-1
M. Feickert, B.B. Burckhardt
Institute of Clinical Pharmacy and Pharmacotherapy, Heinrich Heine University, Dusseldorf, Germany
P 377antibiotics analyses in vinasse from sugarcane ethanol fuel production
J.J. da Silva1, B.F. da Silva1, M.V. Boldrin Zanoni1, M. Gros2, M. Petrovic2, N. Ramos Stradiotto1,3,4
1São Paulo State University (Unesp), Institute of Chemistry (IQ), Araraquara, Brazil; 2Catalan Institute for Water Researsh (ICRA), Girona, Spain; 3São Paulo State University (Unesp), Bioenergy Research Institute (IPBEN), Araraquara, Brazil; 4Fluminense Federal University (UFF), School of Industrial Metallurgical Engineering (EEIMVR), Volta Redonda, Brazil
P 378Upgrading of amino acid analysis using hydrophilic interaction chromatography coupled with isotope-dilution mass spectrometry for accurate protein/peptide quantification
M. Kato*, S. Eyama, M. Yoshioka
NMIJ/AIST, Tsukuba, Japan
P 379Analysis of β-lactam antibiotics in wastewater at sub ppb level using liquid chromatography combined with electrospray tandem mass spectrometry
W. Coppes1, B. Ozalp1, A.L.L. Duchateau1, W. Rensink2
1DSM Biotechnology Center, DSM Food Specialties BV, Delft, The Netherlands; 2Centrient Pharmaceuticals, Delft, The Netherlands

LC/MS and hyphenation - SFC - MS

P 380OPTIMISATION OF A SFC-ESI-MS METHOD FOR NON-TARGET SCREENING OF SEDIMENT SAMPLES USING A D-OPTIMAL DESIGN
J.S. Lübeck, G. Tomasi, J.H. Christensen
Department of Plant and Environmental Sciences, Faculty of Science, University of Copenhagen, Frederiksberg C, Denmark
P 381SUPERCRITICAL FLUID CHROMATOGRAPHY HYPHENATED TO ELECTRON IONIZATION MASS SPECTROMETRY TO ENHANCE IDENTIFICATION RELIABILITY OF SEMI-VOLATILE COMPOUNDS
R. La Tella1,2, F. Rigano1, P. Dugo1,2,3, L. Mondello1,2,3
1Chromaleont s.r.l., c/o Dipartimento di Scienze Chimiche, Biologiche, Farmaceutiche ed Ambientali”, University of Messina, Italy; 2Dipartimento di “Scienze Chimiche, Biologiche, Farmaceutiche ed Ambientali”, University of Messina, Italy; 3Department of Medicine, University Campus Bio-Medico of Rome
P 382Chiral deschloroketamine metabolomics in rat tissue using gradient SFC-MS/MS

N. Kolderová1, M. Kuchař1,2, P. Frühauf3, T. Páleníček4, M. Kohout5
1Forensic laboratory of biologically active substances, University of Chemistry and Technology Prague, Czech Republic; 2Department of Chemistry of Natural Compounds, University of Chemistry and Technology Prague, Czech Republic; 3Department of Analytical Chemistry, University of Vienna, Austria; 4National Institute of Mental Health, Klecany, Czech Republic; 5Department of Organic Chemistry, University of Chemistry and Technology Prague, Czech Republic
P 383Simultaneous detection of nitrosamines and other sartan-related impurities in active pharmaceutical ingredients by supercritical fluid chromatography
S. Schmidtsdorff, A. Schmidt, M. Stanic
Chromicent GmbH, Berlin, Germany
P 384Analysis of exopolysaccharides from archaeal biofilms by supercritical fluid chromatography coupled to mass spectrometry
M. Meyer1,2, L. Kuschmierz3, B. Siebers3, J. Wingender4, O.J. Schmitz1,2
1Applied Analytical Chemistry (AAC), University of Duisburg-Essen, Germany; 2Teaching and Research Center for Separation (TRC), University of Duisburg-Essen, Germany; 3Molecular Enzyme Technology and Biochemistry (MEB), University of Duisburg-Essen, Essen, Germany; 4Aquatic Microbiology, University of Duisburg-Essen, Germany
P 385SFC-MS/MS of phase-1 metabolism of propranolol using chiral sub-2 µm particle columns
L.C. Harps1, J.J. Felix2, M.K. Parr1
1Freie Universität Berlin, Institute of Pharmacy, Pharmaceutical & Medicinal Chemistry, Berlin, Germany; 2Freie Universität Berlin, Institute of Pharmacy, Core Facility BioSupraMol, Berlin, Germany
P 386Determination of polar and ionic drugs and metabolites – systematic investigations for simultaneous analysis utilizing SFC-MS/MS
M. Xhaferaj1,2, E. Nägele2, M.K. Parr1
1Institute of Pharmacy, Freie Universität Berlin, Germany; 2Agilent Technologies, Waldbronn, Germany
P 387determination of pyrrolizidine alkaloids in plant material using sfc-ms/ms
A. Grüning1, G.J. Schad1, J. Stenzler2, R. Wong3
1Shimadzu Europa GmbH, Duisburg, Germany; 2Shimadzu Deutschland GmbH, Duisburg, Germany; 3Shimadzu United Kingdom, Centre of Excellence, Mill Court, Milton Keynes, United Kingdom
P 388Analysis of the HepG2 lipidome by means of supercritical fluid chromatography coupled with IM qTOF MS(/MS)
K. Rentmeister, P. Wittenhofer, O.J. Schmitz, S.W. Meckelmann
Applied Analytical Chemistry, University of Duisburg-Essen, Essen, Germany; Teaching and Research Centre for Separation, Essen, Germany

Fundamentals - Big data/Machine learning

P 389Statistical analysis of isocratic chromatographic data using Bayesian hierarchical modeling
A. Kamedulska, Ł. Kubik, P. Wiczling
Department of Biopharmacy and Pharmacokinetics, Faculty of Pharmacy, Medical University of Gdańsk, Poland
P 390Modeling the impact of experimental parameters and molecular structures on APCI signals’ intensities of selected antipsychotics using gradient boosted trees
J. Krmar1, J. Stojković2, M. Džigal2, A. Protić1, N. Maljurić1, M. Zečević1, B. Otašević1
1Department of Drug Analysis, University of Belgrade – Faculty of Pharmacy, Belgrade, Serbia; 2University of Belgrade – Faculty of Pharmacy, Belgrade, Serbia

Fundamentals - Characterization of HPLC stationary phases

P 391A NEW CONCEPT OF END-CAPPING FOR REVERSED PHASE SILICA MATERIAL: CONVERSION OF SILANOL GROUPS TO SILOXANE BOND BY HEATING
N. Nagae1, E. Shearer2, T. Tuskamoto1
1ChromaNik Technologies Inc. Osaka, Japan; 2BioNik Inc. Fuji, Japan
P 392STABILITY OF HYDROPHILIC INTERACTION LIQUID CHROMATOGRAPHY (HILIC) STATIONARY PHASES UNDER LOW AND HIGH PH TESTING CONDITIONS
K. Berthelette
Chemistry Research and Development, Waters Corporation, Milford, United States
P 393Α COMPARISON BETWEEN C18 AND PENTAFLUOROPHENYL COLUMNS FOR SEPARATION A MIXTURE OF AROMATIC COMPOUNDS (PHENOLS, ANILINES AND BENZENES)
C. Zisi, M. Diamanta, A. Pappa-Louisi
Department of Chemistry, Aristotle University of Thessaloniki, Greece
P 394EFFECTS OF GAMMA IRRADIATION AND COMPARISON OF DIFFERENT EXTRACTION METHODS ON SESQUITERPENE LACTONE YIELDS FROM THE MEDICINAL PLANT THAPSIA GARGANICA L. (APIACEAE)
F. Benkaci-Ali1, A.M. M. Ibrahim2, H.T. Simonsen3
1Laboratory of Organic and Functional Analysis, Faculty of Chemistry, University of Sciences and Technologies Houari Boumediène, USTHB, Bâb-Ezzouar, Algiers, Algeria; 2Department of Agro-Alimentary Technology and Humane nutrition, Laboratory of Chemistry, Agronomic National High School, El-Harrach, Algiers, Algeria; 3Department of Biotechnology and Biomedicine, Technical University of Denmark, Kgs. Lyngby, Denmark
P 395A NEW CONCEPT OF END-CAPPING FOR REVERSED PHASE SILICA MATERIAL: REMAINING OF WATER-SOLVATED SILANOL GROUPS
E. Shearer1, N. Nagae2, T. Tuskamoto2
1BioNik Inc., Fuji, Japan; 2ChromaNik Technologies Inc., Osaka, Japan
P 396COMPARISON OF THE PERFORMANCE OF HILIC COLUMNS
M.C. Garcia-Alvarez-Coque, E. Peris-Garcia, R. Burgos-Gil, J.J. Baeza-Baeza, M.J. Ruiz-Angel
Department of Analytical Chemistry, University of Valencia, Burjassot, Spain
P 397STUDY OF THE SURFACE PROPERTIES AND APPLICATION POTENCY OF THE AMINO ACID- AND PEPTIDE-SILICA STATIONARY PHASES IN LC
M. Skoczylas, S. Bocian, B. Buszewski
Chair of Environmental Chemistry & Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus, University in Toruń, Poland
P 398THE ROLE OF PHASE CHEMISTRY ON ANTIBODY SEPARATIONS: AN IMPORTANT CONSIDERATION OR AN AFTERTHOUGHT?
C.E. Muraco1, P. Lewits2, M. Ye1
1Merck KGaA, Liquid Separations R&D, Bellefonte, Pennsylvania, USA; 2Merck KGaA, Global Franchise Management, Darmstadt, Germany
P 399Investigations into reversed phase chromatography peptide separation systems: Application of a peptide-based column characterisation database for the selection of liquid chromatographic stationary phases
J.K. Field1, M.R. Euerby2, P. Petersson3
1,2Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, UK; 3Analysis and Characterisation Technology, Novo Nordisk A/S, Copenhagen, Denmark
P 400Retention-based description of dopamine metabolism pathways
M. Komendová1, L.F. Ribeiro2, J. Urban1
1Department of Chemistry, Faculty of Science, Masaryk University, Brno, Czech Republic; 2Institute of Chemistry, University of São Paulo, Brazil
P 401The Influence of Linker’s Structure on the Performance of Novel HILIC Silica- and PS-DVB-based Stationary Phases
A. Popov, A. Chernobrovkina, A. Zatirakha, A. Smolenkov, O. Shpigun
Lomonosov Moscow State University, Moscow, Russia
P 402Assessment of bed and particle integrity of size-exclusion columns packed with sub-2-µm particles
J. De Vos1, G. Baron1, H. Terryn2, S. Eeltink1
1Vrije Universiteit Brussel (VUB), Department of Chemical Engineering, Brussels, Belgium; 2Vrije Universiteit Brussel (VUB), Department of Materials and Chemistry, Research group of Electrochemical and Surface Engineering, Brussels, Belgium
P 403Fused Core Particles – Practical considerations of their use in pharmaceutical industry
B. Channer1, S. Lisandrin1, R. James1, C. Florez1, M. Euerby2
1Idorsia Pharmaceuticals Ltd., Allschwil, CH; 2Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, GB
P 404Temperature Effect on Selectivity of Hyperbranched Anion Exchangers toward Organic Acids
A. Uzhel, A. Gorbovskaja, A. Zatirakha, A. Smolenkov, O. Shpigun
Chemistry department, Lomonosov Moscow State University, Moscow, Russia
P 405A Superficially Porous Particle C30 Phase Designed for High Speed HPLC Applications
R.A. Henry1, S.A. Schuster2, C. McHale2, R. Moran2
1Independent Consultant, Penn State University, State College; 2Advanced Materials Technology, Inc., Wilmington
P 406Acceleration of Conventional United States Pharmacopeia Methods with Smaller Column Dimensions and Core Shell Columns
T. Schüßeler*, C. Thiering, P.Jochems, R. Ludwig, G. Schad
Shimadzu Europa GmbH, Duisburg, Germany
P 407Facts about column interchangeability focusing on particle size
B. David
Analytical Laboratory of Formulation Development, Gedeon Richter Plc., Budapest, Hungary
P 408Characterization of RP sorbents by linear solvation energy relationships (LSER)
H. Riering, N. Bilmann
Macherey-Nagel GmbH & Co. KG, Dueren, Germany
P 409Exploring the diversity of octadecylsilane modified 2.7 µm core-shell particles for RP-HPLC
D.C. Ramb, H.R. Wollseifen
MACHEREY-NAGEL GmbH & Co. KG, Dueren, Germany
P 410Theoretical study of the efficiency of HPLC columns with particle size gradient
S. Horváth, E. Farsang, K. Horváth
University of Pannonia, Department of Analytical Chemistry, Veszprem, Hungary

Fundamentals - Chiral separations

P 411RETENTION AND ENANTIOSEPARATION OF DIPEPTIDES ON CHIRAL STATIONARY PHASE WITH BONDED ANTIBIOTIC RISTOCETIN
M. Stepanova, L. Asnin
Department of chemistry and biotechnology, Perm National Research Polytechnic University, Perm, Russian Federation
P 412COMPARATIVE STUDY OF CELLULOSE TRIS(3-CHLORO-5-METHYLPHENYLCARBAMATE) COATED OR COVALENTLY IMMOBILIZED ON SILICA FOR SEPARATION OF ENANTIOMERS IN HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY
T. Khatiashvili1, I. Matarashvili1, T. Farkas2, B. Chankvetadze1
1Institute of Physical and Analytical Chemistry, School of Exact and Natural Sciences, Tbilisi State University, Tbilisi, Georgia; 2Phenomenex Inc., Torrance, USA
P 413ENANTIOSEPARATION OF 4C SUBSTITUTED PYRROLIDINE-2-ONE DERIVATIVES ON POLYSACCHARIDE-BASED COATED CHIRAL STATIONARY PHASES
T. Upmanis, H. Kažoka, N. Orlova, M. Vorona
Latvian Institute of Organic Synthesis, Riga, Latvia
P 414NEW CHIRAL STATIONARY PHASES BASED ON EREMOMYCIN FOR AMINO ACID ENANTIOMERS SEPARATION
N. Sarvin, G. Vasiyarov, S. Staroverov
Lomonosov State University of Moscow, Chemical Department, Moscow, Russia; JSC "BioChemMack S&T", Moscow, Russia; Lomonosov State University of Moscow, Chemical Department, Moscow, Russia
P 415ENANTIOSELECTIVE POTENTIAL OF TEICOSHELL AND VANCOSHELL COLUMNS IN SUPERCRITICAL FLUID CHROMATOGRAPHY
K. Kalíková1, D. Folprechtová1, O. Kozlov2, M.G. Schmid3, D.W. Armstrong4, E. Tesařová1
1Department of Physical and Macromolecular Chemistry, Charles University, Faculty of Science, Prague, Czech Republic; 2Department of Analytical Chemistry, Faculty of Science, P. J. Šafárik University, Košice, Slovak Republic; 3Department of Pharmaceutical Chemistry, Institute of Pharmaceutical Sciences, University of Graz, Austria; 4Department of Chemistry and Biochemistry, University of Texas at Arlington, USA
P 416Three-dimensional chiral HPLC analysis of extraterrestrial amino acids in carbonaceous chondrites
A. FURUSHO1, T. AKITA1, M. MITA2, H. NARAOKA3, K. HAMASE1
1Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, JAPAN; 2KAGAMI LAB., Shiseido Global Innovation Center, Tokyo, JAPAN; 3Department of Earth and Planetary Sciences, Kyushu University, Fukuoka, JAPAN
P 417Ultra-fast and high-resolution enantioseparations of Nα-FMOC proteinogenic amino acids by means of zwitterionic teicoplanin-based chiral stationary phase made on FPP and SPP silica particles with different porosity
G. Mazzoccanti1, S. Manetto1, O.H. Ismail2, A. Ricci3, W. Cabri3, M. Ye4, M. Catani2, A. Cavazzini2, F. Gasparrini1
1Department of Drug Chemistry and Technology, “Sapienza” University of Rome, Italy; 2Department of Chemistry and Pharmaceutical Sciences, University of Ferrara, Italy; 3Fresenius Kabi iPSUM, Cernusco sul Naviglio (MI), Italy; 4Sigma-Aldrich/Supelco, Bellefonte, United States
P 418Synthesis and evaluation of naphthalene-based chiral strong cation exchangers for chiral separation of basic drugs
J. Herciková, M. Kohout
Department of Organic Chemistry, University of Chemistry and Technology Prague, Czech Republic
P 419Investigation of retention mechanism of chiral separations in supercritical fluid chromatography
E. Horváth1, Y. Koshman1, A. Felinger1,2,3
1Department of Analytical and Environmental Chemistry, University of Pécs, Hungary; 2MTA-PTE Molecular Interactions in Separation Science Research Group, Pécs, Hungary; 3Institute of Bioanalysis, University of Pécs, Hungary
P 420Investigation the retention mechanism of alkaloid-based chiral zwitterionic HPLC stationary phase via nonlinear chromatography
I. Bacskay1,2, R. Kulágin2, W. Lindner3, A. Felinger1,2,4
1MTA–PTE Molecular Interactions in Separation Science Research Group, Pécs, Hungary; 2Department of Analytical and Environmental Chemistry and Szentágothai Research Center, University of Pécs, Hungary; 3Institute of Analytical Chemistry, University of Vienna, Austria; 4Faculty of Medicine, Institute of Bioanalytics, University of Pécs, Hungary
P 421Development and validation of a HPLC method for the enantiomeric purity testing of timolol maleate on ovomucoid chiral stationary phase
M. Mitrović, A. Malenović, B. Otašević, A. Protić, M. Zečević
Department of Drug Analysis, University of Belgrade-Faculty of Pharmacy, Belgrade, Serbia
P 422The Electrostatic Potential Source Function (EPSF): a valuable tool to study enantioseparations involving sigma-holes as recognition sites
C. Gatti1,2, P. Peluso3, V. Mamane4, S. Cossu5
1Istituto di Scienze e Tecnologie Molecolari ISTM-CNR, Milano, Italy; 2Istituto Lombardo Accademia di Scienze e Lettere, Milano, Italy; 3Istituto di Chimica Biomolecolare ICB CNR, Sassari, Italy; 4Institut de Chimie de Strasbourg, UMR 7177, Equipe LASYROC, Strasbourg, France; 5DSMN, Università Ca' Foscari Venezia, Italy
P 423Practical applicability of sequential analysis of reverse phase liquid chromatography (RPLC) and supercritical fluid chromatography (SFC) with an LC/SFC switching system
S. Horie, Y. Hayakawa
Shimadzu Corporation, Kyoto, Japan
P 424exploring the design space for enantiomeric resolution of terbutaline drug and its sulfate conjugate metabolites using design of experiment approach
D. Abou-Shady1, R.S. Hanafi1, M.K. Parr2
1Unit of Instrumental Analysis, Department of Pharmaceutical Chemistry, Faculty of Pharmacy and Biotechnology, German University in Cairo, Egypt; 2Institute of Pharmacy, Freie Universität Berlin, Germany
P 425Sample Loadability on Coated and Immobilized Polysaccharide-Based CSPs
S. Anderson, E. Franklin, M. Wilcox
Regis Technologies Inc., Morton Grove, USA
P 426Observation of Peculiar Peak Shapes in the Direct and Indirect Enantioseparation of Proline – Cinchona Alkaloid Derivatives
R. Reischl1, M. Kohout2, M. Schubert1, W. Lindner3
1Department of Biosciences, University of Salzburg, Austria; 2Department of Organic Chemistry, University of Chemistry and Technology Prague, Czech Republic; 3Department of Analytical Chemistry, University of Vienna, Austria
P 427Comparative study of enantioseparations of natural and unnatural dipeptides on Cinchona alkaloid-based chiral stationary phases
A. Péter1, A. Bajtai1, G. Tóth2, W. Lindner3, G.K.E. Scriba4, I. Ilisz1
1Institute of Pharmaceutical Analysis, Interdisciplinary Excellence Centre, University of Szeged, Hungary; 2Deapartment of Medical Chemistry, University of Szeged, Hungary; 3Department of Analytical Chemistry, University of Vienna, Austria; 4Department of Pharmaceutical and Medicinal Chemistry, University of Jena, Germany
P 428Simultaneous enantiomeric separation and binding constants determination by affinity capillary electrophoresis using human serum albumin
R. Ratih1,2, M. Stein1, M. Asmari1, S. El Deeb1
1Institute of Medicinal and Pharmaceutical Chemistry, TU Braunschweig, Germany; 2Faculty of Pharmacy, the University of Surabaya, Indonesia
P 429Capillary electrophoresis for separation and evaluation of enantiospecific interactions of chiral helical cations
H.R. Talele, L. Severa, V. Kasicka
Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences, Prague, Czech Republic
P 430Enantiorecognition of Dihydropyrimidine Antagonists of Adenosine A2B Receptor by Pirkle-type and Teicoplanin-based chiral stationary phases
I. Bolognino1, C.D. Altomare1, S. Cellamare1, A. Carrieri1, R. Caliandro3, D. Belviso3, E. Sotelo2, M. Majellaro2
1Dipartimento di Farmacia-Scienze del Farmaco Universitty of Bari “Aldo Moro”, Italy; 2Centro Singular de Investigacioìn en Química Bioloìgica y Materiales Moleculares (CIQUS), and ‡Departamento de Química Orgaìnica, Facultad de Farmacia, Universidade de Santiago de Compostela, Spain; 3Institute of Crystallography- CNR- Via Giovanni Amendola, Bari, Italy

Fundamentals - Column design and stationary phase

P 431IONIC LIQUIDS-BASED STATIONARY PHASES: DESIGN, PREPARATION AND FURTHER USED FOR HIGH-EFFICIENCY HPLC SEPARATION
X. Qiao
College of Pharmaceutical Sciences, Hebei University, Baoding, China
P 432CORE-SHELL METAL-ORGANIC FRAMEWORKS AS STATIONARY PHASE FOR REVERSED-PHASE/ HYDROPHILIC INTERACTION MIXED-MODE CHROMATOGRAPHY
X. Qin1, X. Qiao2, X. Li2
1College of Chemistry & Environmental Science, Hebei University, Baoding, China; 2College of Pharmaceutical Sciences, Hebei University, Baoding, China
P 433CHIRAL STATIONARY PHASES BASED ON SMALL MOLECULES: DEVELOPMENT, ENANTIORESOLUTION EVALUATION AND CHIRAL RECOGNITION MECHANISMS
Y. Zaw Phyo1,2, J. Teixeira3, S. Cravo2,3, M.E. Tiritan2,3,4, A.M.S. Silva5, M.M.M. Pinto2,3, A. Kijjoa1,2, C Fernandes2,3
1Instituto de Ciências Biomédicas Abel Salazar, Universidade do Porto, Portugal; 2Centro Interdisciplinar de Investigação Marinha e Ambiental (CIIMAR), Universidade do Porto, Portugal; 3Laboratório de Química Orgânica e Farmacêutica, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, Portugal; 4CESPU, Instituto de Investigação e Formação Avançada em Ciências e Tecnologias da Saúde, Gandra, Portugal; 5Departamento de Química & QOPNA, Universidade de Aveiro, Portugal
P 434DEVELOPMENT OF MONOLITHIC SILICA CAPILLARY COLUMNS FOR HIGH RESOLUTION SEPARATION OF INTACT PROTEINS
H. Kobayashi, H. Wada
Shinwa Chemical Industries, Ltd., Kyoto, Japan
P 435AN INNOVATIVE SOLUTION TO IMPROVING LC/MS ANALYSES OF METAL-SENSITIVE POLAR COMPOUNDS – FROM MOBILE PHASE IMPROVEMENTS, TO UNIQUE COLUMN CONFIGURATIONS, TO A PURPOSE-BUILT LC SYSTEM
A. Mack, W. Long, L. Subbarao, R.J. Fu, A. Borowiak, A. Bivens
Agilent, Wilmington, USA
P 436GENERAL ALGORITHM OF STATIONARY PHASE DESIGN AND SELECTIVITY ADJUSTMENT FOR ORGANIC ACID ANALYSIS BY SUPPRESSED ION CHROMATOGRAPHY
A. Zatirakha, A. Uzhel, A. Gorbovskaya, A. Smolenkov, O. Shpigun
Chemistry Department, Lomonosov Moscow State University, Moscow, Russian Federation
P 437DESIGNING A NEW PARTICLE TECHNOLOGY AND PH GRADIENT MOBILE PHASE CONCENTRATES FOR ROBUST, HIGH RESOLUTION CHARGE VARIANT ANALYSIS OF MABS
M. Xu, Q. Wang, S.C. Rzewuski, H. Yang, S. Koza, M.F. Morris, J. McLaughlin, S. Shiner, B. Niu, M. Lauber
Waters Corporation, Milford, USA
P 438SYNTHESIS OF A HYBRID MONOLITHIC CAPILLARY COLUMN BASED ON METHACRYL SUBSTITUTED POLYHEDRAL OLIGOMERIC SILSESQUIOXANE (POSS-MA) AND 1,12-DI (3-VINYLIMIDAZOLIUM) DODECANE DIBROMIDE IONIC LIQUID
F. Pires, L.W. Hantao, C.B.G. Bottoli
Institute of Chemistry, University of Campinas – UNICAMP, Campinas, Brazil
P 439SYNTHESIS AND CHARACTERIZATION OF ZWITTERIONIC-TYPE RESIN FOR HIGH PERFORMANCE LIQUID CHROMATOGRAPHY AND ITS APPLICATION TO AMINO ACIDS SEPARATION
Y. Takagai1, K. Yamazoe1, M. Butsugan2, C. Ohnuma2, W.L. Hinze3
1Faculty of Symbiotic Systems Science, Cluster of Science and Technology, Fukushima University, Fukushima, Japan; 2Hitachi Chemical Techno Service Co. Ltd., Hitachi, JAPAN; 3Department of Chemistry, Wake Forest University, Winston-Salem, USA
P 440Effect of column packing procedure on the column ends structure and on bed heterogeneity - experiments with flow-reversal
D. Zelenyánszki1, N. Lambert2, F. Gritti3, A. Felinger4
1Department of Analytical and Environmental Chemistry and Szentágothai Research Center, University of Pécs, Hungary; 2MTA–PTE Molecular Interactions in Separation Science Research Group, Pécs, Hungary; 3Waters Corporation, Instrument/Core Research/Fundamental Milford, USA; 4Department of Analytical and Environmental Chemistry and Szentágothai Research Center, University of Pécs, Hungary; MTA–PTE Molecular Interactions in Separation Science Research Group, Pécs, Hungary; Institute of Bioanalysis, Medical School, University of Pécs, Hungary
P 441Selective separation of binding-active molecules on thyroid-hormone-receptor by using molecularly imprinted polymers
S. YAMAMOTO1, T. NAITO1, D. NAKAJIMA2, T. KUBO1, K. OTSUKA1


1Graduate School of Engineering, Kyoto University, Katsura, Japan; 2Center for Health and Environmental Risk Research, National Institute for Environmental Studies, Onogawa, Japan
P 442Retention characteristics of carbon-materials coated columns for halogenated aromatic compounds in liquid chromatography
E. KANAO*, T. NAITO, T. KUBO, K. OTSUKA
Kyoto University, Department of Material Chemistry, Graduate School of Engineering, Kyoto, Japan
P 443Development of high-porosity monolithic columns featuring sub-micron globules and macropores and structural homogeneity characterization via nano-liquid chromatography
J.L. Dores-Sousa, S. Eeltink
Vrije Universiteit Brussel (VUB), Department of Chemical Engineering, Brussels, Belgium
P 444Column-in-Valve Designs to Minimize Extra-Column Band Broadening and Maximize Throughput Speed
V. Pepermans1, B. Degreef1, T.M. Rerick2, G.S. Weber2, G. Desmet1
1Department of Chemical Engineering, Vrije Universiteit Brussel, Belgium; 2Department of Chemistry, University of Pittsburgh, USA
P 445PolyHIPEs-based stationary phases as innovative supports for the immobilization of valuable macromolecules for pharmaceutical applications
M. Corti, F. Rinaldi, G. Tripodo, G. Massolini, E. Calleri
Department of Drug Sciences, University of Pavia, Italy
P 446A comprehensive investigation of the peak capacity for the reversed-phase gradient liquid-chromatographic analysis of intact proteins using a polymer-monolithic capillary column
A. Fernández-Pumarega1, J.L. Dores-Sousa2, S. Eeltink3
1Departament d’Enginyeria Química i Química Analítica and Institut de Biomedicina (IBUB), Facultat de Química, Universitat de Barcelona, Spain; 2Vrije Universiteit Brussel (VUB), Department of Chemical Engineering, Brussels, Belgium; 3Vrije Universiteit Brussel (VUB), Department of Chemical Engineering, Brussels, Belgium
P 447Evaluation of Polymer-based Stationary Phases for Temperature Responsive Liquid Chromatography
M. Baert1, F. Du Prez2, F. Lynen1
1Separation Science Group, Department of Organic and Macromolecular Chemistry, Faculty of Sciences, Ghent University, Belgium; 2Polymer Chemistry Research Group, Department of Organic and Macromolecular Chemistry, Faculty of Sciences, Ghent University, Belgium
P 448Advantages of Superficially Porous Particles (SPP) for Faster HPLC and UHPLC Separations
S.J. Rosenberg1, R.A. Henry2, S.A. Schuster1, C. McHale1, A.F. Harron1
1Advanced Materials Technology, Inc., Wilmington, Delaware, United States of America; 2Independent Consultant, Penn State University, State College, Pennsylvania, United States of America
P 449Study on relationship between polymer structures on silica particles and the separation characteristics of the corresponding HPLC columns for hydrophilic interaction chromatography
A. Taniguchi, K. Ito, S. Tamura, T. Ikegami
Faculty of Molecular Chemistry and Engineering, Kyoto Institute of technology, Kyoto, Japan
P 450New selectivities via ligand immobilization
B. Peters1, T. Kupfer2, G. Jung1, P. Knoell1, P. Lewits3
1Merck KGaA, Instrumental Analytics R&D, Darmstadt, Germany; 2Merck KGaA, Quality Specialities, Darmstadt, Germany; 3Merck KGaA, Instrumental Analytics & Analytical Chromatography, Darmstadt, Germany
P 451Evaluation of a new SEC column for the UHPLC analysis of biopolymers
R. Roemling1, P. Endres1, A. Chakrabarti2, E. Müller1
1Tosoh Bioscience GmbH, Griesheim, Germany; 2Tosoh Bioscience LLC, King of Prussia, USA
P 452The use of polyurethane foam for the heat insulation of liquid chromatographic columns
N. Lambert1, A. Felinger3
1University of Pécs, Department of Analytical and Environmental Chemistry, Pécs, Hungary; 2The Dow Chemical Company, The Hague Area, Netherlands; 3University of Pécs, Department of Analytical and Environmental Chemistry, Pécs, Hungary

Fundamentals - HP-TLC

P 453HPLC Mobile Phase Packaging, Performance and Laboratory Productivity
A.M. Conlon, S. Bhattacharya, S. Brookes-Bertolatus, D. Clain, E. Oliver, J. Pardiwala, A. Kaczmarski, S. Roemer
Thermo Fisher Scientific - Laboratory Chemicals Division
P 454Detection of estrogen active nonylphenols by planar solid phase extraction–planar yeast estrogen screen in surface waters
D. Schick, C. Oellig
Institute of Food Chemistry, University of Hohenheim, Stuttgart, Germany
P 455From Validation to Uncertainty of Measurement of Quantitative HPTLC Methods in Foodstuff Industry
1P. Bernard-Savary, 2J.M. Roussel, 3I. Mouly
1Chromacim, Pommiers-la-Placette, France; 2Consultant, Mâcon, France; 3Lesaffre Développement, Marcq-en-Baroeul, France
P 456Modern bioautography - a fast analytical tool to discover active compounds in plant extracts used for cosmetics
M. Oberle*1, J. Engemann1, I. Klingelhöfer2, G. Morlock2
1Merck KgaA, Life Science, Darmstadt, Germany; 2Justus Liebig University Giessen, Institute of Nutritional Science, and Interdisciplinary Research Center, Giessen, Germany

Fundamentals - Mass transfer phenomena

P 457PROTEIN CHROMATOGRAPHY WITH POLY(ALLYLAMINE)-GRAFTED SEPHAROSE FF
M. Li, Y. Wu, X. Dong, Y. Sun
Tianjin University
P 458Detailed investigation of mesopore diffusion in liquid chromatography
H. Song1, Y. Vanderheyden2, D. Cabooter1, G. Desmet2
1KU Leuven, Department of Pharmaceutical & Pharmacological Sciences, Leuven, Belgium; 2Free University Brussels, Department of Chemical Engineering, Brussel, Belgium

Fundamentals - Multidimensional chromatography

P 459POLYPROPYLENE CAPILLARY-CHANNELED POLYMER FIBER COLUMN AS THE SECOND DIMENSION IN COMPREHENSIVE, TWO-DIMENSIONAL ANALYSIS OF COMPLEX PROTEIN MIXTURES AND E. COLI CELL LYSIS
W. Lei, M.R. Kenneth
Department of Chemistry, Clemson University, USA
P 460CHIRAL X ACHIRAL MULTIDIMENSIONAL LIQUID CHROMATOGRAPHY. APPLICATIONS TO THE ENANTIOSEPARATION OF DNP AMINO ACIDS IN HONEY SAMPLES OF DIFFERENT REGIONS OF MENDOZA (ARGENTINA).
A. Acquaviva1, G. Siano2, M. Filgueira3, C. Castells4
1LIDMA (Laboratorio de Investigación y Desarrollo de Métodos Analíticos), Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Argentina; 2Instituto de Investigación en Señales, Sistemas e Inteligencia Computacional, sinc(i) FICH/UNL-CONICET, Santa Fe, Argentina; 3Industrial Biosciences - DuPont, EE. UU.; 4LIDMA (Laboratorio de Investigación y Desarrollo de Métodos Analíticos), Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Argentina
P 461A SELECTIVE COMPREHENSIVE 2D-LC METHOD FOR THE QUALITY CONTROL OF SYNTHETIC THERAPEUTIC PEPTIDES
R. Karongo, M. Lämmerhofer
Pharmaceutical (Bio-)Analysis, Institute of Pharmaceutical Sciences, Eberhard-Karls-University Tuebingen, Germany
P 462COMPREHENSIVE TWO-DIMENSIONAL LIQUID CHROMATOGRAPHY COUPLED TO MASS SPECTROMETRY FOR QUALI-QUANTITATIVE ANALYSIS OF BIO-OILS AQUEOUS PHASES FROM DIFFERENT BIOMASSES
K. Arena1, E. Lazzari2, E. Bastos-Camarao2,3, P. Dugo1,4,5, L. Mondello1,4,5, A. Cifuentes6, M. Herrero6
1Dipartimento di Scienze chimiche, Biologiche, Farmaceutiche ed Ambientali, University of Messina, Italy; 2Institute of Chemistry, UFRGS, Porto Alegre, RS, Brazil; 3Department of Industrial Biotechnology, Tiradentes University, Sergipe, Brazil; 4Chromaleont s.r.l., c/o Dipartimento di Scienze Chimiche, Biologiche, Farmaceutiche ed Ambientali, University of Messina, Italy; 5Department of Medicine, University Campus Bio-Medico of Rome, Italy; 6Laboratory of Foodomics, Institute of Food Science Research (CIAL, CSIC-UAM), Madrid, Spain
P 463Improved pharmaceutical impurity determination via comprehensive 2D-LC temperature responsive x reversed phase liquid chromatography
K. Wicht1, M. Baert1, S. Krieger2, N. von Doehren3, A. de Villiers4, F. Lynen1

1Department of Organic and Macromolecular Chemistry, Ghent University, Belgium; 2Agilent Technologies, Waldbronn, Germany; 3Agilent Technologies, Middelburg, Netherlands; 4Department of Chemistry, University of Stellenbosch, Matieland, South Africa
P 464Development of a generic two-dimensional achiral-chiral multiple heart-cutting platform for the analysis of pharmaceutical impurities
R.S. Hegade, K. Chen, J.-P. Boon, M. Hellings, F. Lynen
Separation Science Group, Department of Organic and Macromolecular Chemistry, Faculty of Sciences, Ghent University, Belgium; Janssen Pharmaceutical Companies of Johnson & Johnson, Beerse, Belgium
P 465A new method study based on multidimensional high performance liquid chromatography for separation and analysis of protein complexes
M. Gao, X. Wang, X. Zhang
Chemistry Department, Fudan University, Shanghai, China

Fundamentals - Other

P 466STRATEGIES FOR THE TRANSFER OF LIQUID CHROMATOGRAPHIC METHODS BETWEEN DIFFERENT INSTRUMENTS
M. Grübner, C. Paul, M. De Pra, F. Steiner
Thermo Fisher Scientific, Germering, Germany
P 467A Pareto-optimality perspective to study the kinetic performance of different separation modes in liquid chromatography
T. Themelis, J. De Vos, S. Eeltink
Vrije Universiteit Brussel (VUB), Department of Chemical Engineering, Brussels, Belgium
P 468The influence of guard columns on the chromatographic separation
J. Sander, C. Thiering, P. Jochems, R. Ludwig, G. Schad, T. Schüßeler
Shimadzu Europe GmbH, Duisburg, Germany
P 469Ultra-fast Screening of Aliphatic Acids in Human Plasma by Trapped Ion Mobility Spectrum
Y. Wang, Y. Han*
State Key Laboratory of Heavy Oil Processing, China University of Petroleum, Beijing, China
P 470Assessing the impact of increased system volume on peak shape for high organic diluent samples using U(H)PLC
C. Henry1, P. Rainville2, P. McConville2, P Hong2
1Waters Corporation, Wilmslow, UK; 2Waters Corporation, Milford, USA
P 471Improvement of quantitative performance for photodegradable compounds using UV cut-off filter on photodiode array detector
Y. Watabe, A. Morita, D. Vecchietti, T. Uchikata, H. Terada, K. Matsumoto
Shimadzu Corporation., Kyoto, Japan
P 472Determination of solubility of poorly water-soluble drugs in different lipid excipients and lipid-based formulations – Method development and validation
J. Krause, K. Baumann
Technische Universität Braunschweig, Institute of Medicinal and Pharmaceutical Chemistry, Braunschweig, Germany
P 473COMPARISON OF LC-MS AND CE-MS FOR THE ANALYSIS OF IMMUNSTIMULANT BACTERIAL MEMBRANE GLYCOLIPIDS
V. Sándor1, A. Kilár1, B. Berkics1, B. Kocsis2, F. Kilár1, Á. Dörnyei3
1Institute of Bioanalysis, Medical School, University of Pécs, Hungary; 2Department of Medical Microbiology and Immunology, Medical School, University of Pécs, Hungary; 3Department of Analytical and Environmental Chemistry, and Szentágothai Research Center, University of Pécs, Hungary

Fundamentals - Retention and selectivity

P 474THE INFLUENCE OF ONE-DIMENSIONAL PARAMETERS IN THE SHAPE SELECTIVITY IN REVERSED-PHASE LIQUID CHROMATOGRAPHY FOR CATA-CONDENSED POLYCYCLIC AROMATIC HYDROCARBONS
J. Oña-Ruales
Department of Chemical and Materials Engineering, Nazarbayev University, Astana, Kazakhstan
P 475SOLVENT CONCENTRATION PULSES TO IMPROVE RETENTION MODELLING IN LIQUID CHROMATOGRAPHY
J.R. Torres-Lapasio, J.A. Navarro-Huerta, A. Gisbert-Alonso, M.C. Garcia-Alvarez-Coque
Department of Analytical Chemistry, University of Valencia, Burjassot, Spain
P 476DEVELOPMENT OF A GENERAL RETENTION MODEL FOR LIQUID CHROMATOGRAPHY
J.J. Baeza-Baeza, M.C. Garcia-Alvarez-Coque
Department of Analytical Chemistry, University of Valencia, Burjassot, Spain
P 477EVALUATION OF THE INFORMATION QUALITY IN GRADIENT EXPERIMENTAL DESIGNS
J.A. Navarro-Huerta, J.R. Torres-Lapasió, S. López-Ureña, M.C. García-Alvarez-Coque
Department of Analytical Chemistry (University of Valencia), Burjassot, Spain
P 478NATURE OF MOBILE PHASE PREPARATION AND ELECTROLYTE ADDITION ON RETENTION AND SELECTIVITY IN HYDROPHILIC INTERACTION LIQUID CHROMATOGRAPHY
C.B. Craven, C.A. Lucy*
Department of Chemistry, Gunning/Lemieux Chemistry Centre, University of Alberta, Edmonton, Alberta
P 479LOCALISED QUANTITATIVE STRUCTURE-RETENTION RELATIONSHIP MODELLING FOR RAPID METHOD DEVELOPMENT IN REVERSED-PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
S.H. Park1, M. De Pra1, P.R. Haddad2, S. Grosse1, C.A. Pohl3, F. Steiner1
1Thermo Fisher Scientific, Germering, Bavaria, Germany; 2Australian Centre for Research on Separation Science (ACROSS), School of Physical Sciences-Chemistry, University of Tasmania, Hobart, Australia; 3Thermo Fisher Scientific, Sunnyvale, USA
P 480RETENTION-MECHANISM STUDY IN HYDRODYNAMIC CHROMATOGRAPHY
N. Abdulhussain1, D. van Herwerden1, T.D. Adamopoulou1, B.W.J. Pirok1, R. Peters2, P.J. Schoenmakers1
1Van 't Hoff Institute for Molecular Science (HIMS), Faculty of Science, University of Amsterdam, The Netherlands; 2DSM Coating Resins, The Netherlands
P 481Analytical separation of carboxylic acids and inorganic anions on cryptand-based ion exchange stationary phase
D. Lukács, K. Horváth, P. Hajós
Department of Analytical Chemistry, University of Pannonia, Veszprém, Hungary
P 482Unified pH values of liquid chromatography mobile phases
A. Heering1, R. Born1, S. Tšepelevitš1, L. Jalukse1, J. Liigand1, A. Kruve1, D. Himmel2, I. Krossing2, M. Rosés3, I. Leito1
1Institute of Chemistry, University of Tartu, Estonia; 2Institute for Inorganic and Analytical Chemistry and Freiburger Materialforschungszentrum (FMF), Albert-Ludwigs-Universität Freiburg, Germany; 3Departament de Química Analítica, Universitat de Barcelona, Spain
P 483Structure assisted impurity profiling for rapid method development in liquid chromatography
S. Schmidtsdorff, A.H. Schmidt, M.K. Parr, M. Stanic
Chromicent GmbH, Berlin, Germany; Freie Universität Berlin, Institute of Pharmacy, Berlin, Germany
P 484lc simulation software and qbd approach employment for complex chromatographic method development
E. Gritti1, V. Meli1, G. Boschello1, E. De Dominicis1, R. Arduini2
1Mérieux NutriSciences Research, Resana (TV), Italy; 2S-IN Soluzioni Informatiche Srl, Vicenza, Italy
P 485Determination of the retention factor of ionizable compounds in microemulsion electrokinetic chromatography
A. Fernández-Pumarega, S. Amézqueta, E. Fuguet, M. Rosés
Departament d’Enginyeria Química i Química Analítica and Institut de Biomedicina (IBUB), Facultat de Química, Universitat de Barcelona, Spain
P 486Prediction and Optimization of (co)Polymer Separations
L.E. Niezen1, S.R.A. Molenaar1, B.W.J. Pirok1, H.J.A. Philipsen2, G.W. Somsen3, P.J. Schoenmakers1
1Van 't Hoff Institute for Molecular Science (HIMS), University of Amsterdam, The Netherlands; 2DSM Materials Science Center (DMSC), Geleen, The Netherlands; 3Biomolecular Analysis and Spectroscopy, Amsterdam Institute for Molecules, Medicines and Systems, Vrije Universiteit Amsterdam, The Netherlands

Fundamentals - SFC

P 487COLUMN COUPLING IN SUPERCRITICAL FLUID CHROMATOGRAPHY USING CHIRAL AND ACHIRAL STATIONARY PHASES
P. Riasová1,2, P. Jáč1, M. Polášek1, Y. Vander Heyden2, D. Mangelings2
1Department of Analytical Chemistry, Faculty of Pharmacy in Hradec Králové, Charles University, Czech Republic; 2Department of Analytical Chemistry, Applied Chemometrics and Molecular Modelling, Faculty of Medicine and Pharmacy, Vrije Universiteit Brussel, Belgium
P 488Quantitative analysis of organic light emitting diode materials by supercritical fluid chromatography coupled with mass spectrometry
K. Yoon1, Y. Cho2, H. Moon3, E.H. Lee3, N. Ahn3, S. Kim1,2
1Department of Chemistry, Kyungpook National University, Daegu, South Korea; 2Green-Nano Materials Research Center, Daegu, South Korea; 3Samsung Display, Asan, South Korea
P 489Exploring the limits of nitrous oxide as a tracer in supercritical fluid chromatography
C. Rédei1, A. Felinger1,2,3
1Department of Analytical and Environmental Chemistry, University of Pécs, Hungary; 2MTA-PTE Molecular Interactions in Separation Science Research Group, Pécs, Hungary; 3Institute of Bioanalysis, University of Pécs, Hungary
P 490Competitive adsorption of mobile phase modifiers in supercritical fluid chromatography
Y. Kazmouz1, A. Felinger1,2,3
1Department of Analytical and Environmental Chemistry and Szentágothai Research Center, University of Pécs, Hungary; 2MTA-PTE Molecular Interactions in Separation Science Research Group, University of Pécs, Hungary; 3Institute of Bioanalysis, Medical School, University of Pécs, Hungary
P 491Polyethylene glycol (PEG) characterisation by Supercritical Fluid Chromatography (SFC)
R. Mott, A. Baldaccini, A. Gabe, R. Linke, A. Poulton, K. Treacher
AstraZeneca, Silk Road Business Park, Charter Way, Macclesfield, United Kingdom
P 492Development of a Single Supercritical Fluid Chromatography Method for Glimepiride Impurity Analysis
J. Simeone, P. Hong
Waters Corporation, Milford, USA
P 493Modern Supercritical Fluid Chromatography – Impact of Mobile Phase Composition
M. Enmark1,2, E. Glenne1, M. Lesko1, F. Limé3, J. Samuelsson1, T. Fornstedt1
1Department of Engineering and Chemical Sciences, Karlstad University, Sweden; 2Pharmacognosy, Department of Medicinal Chemistry, Uppsala University, Biomedical Centre, Uppsala, Sweden; 3Nouryon, Separation Products, Bohus, Sweden

Fundamentals - Thermodynamics and separation mechanism

P 494EFFECT OF MOBILE PHASE COMPOSITION ON THERMODYNAMIC PROPERTIES OF ZWITTERIONIC TEICOPLANIN-BASED SUB 2-μm SUPERFICIALLY POROUS PARTICLES FOR ULTRAFAST ENANTIOSEPARATIONS
S. Felletti1, O.H. Ismail1, M. Catani1, C. De Luca1, F. Gasparrini2, A. Cavazzini1

1Department of Chemistry and Pharmaceutical Sciences, University of Ferrara, Italy; 2Deptartment of Drug Chemistry and Technology, “La Sapienza” University of Rome, Italy
P 495Improving separation and peak tailing of basic compounds through dynamic electrostatic repulsion reversed phase (d-ERRP) liquid chromatography
S. Manetto1, G. Mazzoccanti1, O.H. Ismail2, C. Villani1, F. Gasparrini1
1Department of Drug Chemistry and Technology, “Sapienza” University of Rome, Italy; 2Department of Chemistry and Pharmaceutical Sciences, University of Ferrara, Italy
P 496Correlation of microscopic and macroscopic theories of high performance liquid chromatography
A. Somfai, K. Horváth
University of Pannonia, Department of Analytical Chemistry, Veszprém, Hungary
Poster Session

Applications - Anal. characterization of protein biopharmaceuticals

P 1Analytical challenges and strategies related to plant-derived vs mammalian cell-derived antibodies
S. Tengattini1, F. Rinaldi1, G. Massolini1, C. Temporini1, R. Venturi2, C. Pisano2, C. Camponeschi2
1Department of Drug Sciences, University of Pavia, Italy; 2Special Product’s Line S.p.A., Roma, Italy
P 2CVA-MS ANALYSIS TO INVESTIGATE THE EFFECTS OF LEACHABLES FROM SINGLE-USE BIOREACTORS ON MONOCLONAL ANTIBODIES
S. Carillo1, F. Füssl1, N.D. Garcia1, C. Ta1, P.S. Kelly2, N. Barron2, J. Bones2,3
1Characterisation and Comparability Laboratory, NIBRT. Foster Avenue, Mount Merrion, Blackrock, co. Dublin. Ireland; 2National Institute for Cellular Biotechnology, Dublin City University, Glasnevin, Dublin, Ireland; 3School of Chemical and Bioprocess Engineering, University College Dublin, Belfield, Dublin
P 3A NEW CHROMATOGRAPHIC APPROACH TO QUICKLY ASSESS ADCC ACTIVITY OF THERAPEUTIC ANTIBODIES
P. Endres, L. Salim, R. Römling, E. Müller
Tosoh Bioscience GmbH, Griesheim, Germany
P 4ASYMMETRICAL FLOW FIELD-FLOW FRACTIONATION AND SIZE-EXCLUSION CHROMATOGRAPHY FOR STUDYING THE AGGREGATION OF BETA-D-GALACTOSIDASE
I.K. Ventouri1,2, A. Astefanei1, E. Kaal3, G.W. Somsen4, P. Schoenmakers1
1University of Amsterdam, van't Hoff Institute for Molecular Sciences-Analytical Chemistry Group, Amsterdam, The Netherlands; 2TI-COAST, Amsterdam, The Netherlands; 3DSM Biotechnology Center, DSM, Delft, The Netherlands; 4Vrije Universiteit Amsterdam, Division of BioAnalytical Chemistry, Amsterdam, The Netherlands
P 5AN AUTOMATED WORKFLOW FOR ON-COLUMN DIGESTION AND ANALYSIS OF MONOCLONAL ANTIBODIES
M. Nordgren, A. Nägeli, S. Björk, H. Nyhlén, J. Sjögren, F. Olsson
Genovis AB, Lund, Sweden
P 6CHARACTERIZATION OF MONOCLONAL ANTIBODY THERAPEUTICS BY CAPILLARY ELECTROPHORESIS ELECTROSPRAY IONIZATION MASS SPECTROMETRY
K. Müller, L. Stock, H. Stutz
Department of Biosciences, University of Salzburg, Austria; Christian Doppler Laboratory for Innovative Tools for Biosimilar Characterization, Salzburg, Austria
P 7METHOD DEVELOPMENT AND PURIFICATION STRATEGIES OF RAW THERAPEUTIC PEPTIDE MIXTURES BY MEANS OF PREPARATIVE CHROMATOGRAPHY
G. Lievore1, C. De Luca1, S. Felletti1, M. Macis2, A. Ricci2, W. Cabri2, O.H. Ismail1, M. Catani1, M. Morbidelli3, A. Cavazzini1
1Department of Chemical and Pharmaceutical Sciences, University of Ferrara, Italy; 2R&D, Fresenius Kabi iPSUM, Villadose, Rovigo, Italy; 3Dept. of Chemistry and Applied Biosciences, Institute for Chemical and Bioengineering ETH, Zurich, Switzerland
P 8Multi-attribute Analysis of Monoclonal Antibodies by 3D-LC and Q-TOF Mass Spectrometry
G. Vanhoenacker1, I. Vandenheede1, P. Sandra1, K. Sandra1, S. Krieger2, S. Schneider2, U. Huber2
1Research Institute for Chromatography (RIC), Kortrijk, Belgium; 2Agilent Technologies, Waldbronn, Germany
P 9Good peak shape and recovery for phosphorylated compounds – Analysis of nucleotides employing an LC with a fully inert flow path
F. Rieck2, K. Sandra1, J. Vandenbussche1, G. Vanhoenacker1, R. t’Kindt1, P. Sandra1, S. Krieger2, S. Schneider2, U. Huber2
1Research Institute for Chromatography (RIC), Kortrijk, Belgium; 2Agilent Technologies, Waldbronn, Germany
P 10Alternative mobile phases in cation-exchange chromatography applied for separation of monoclonal antibodies
E. Farsang1, A. Murisier2, K. Horváth1, A. Beck3, R. Kormány4, D. Guillarme2, S. Fekete2
1Department of Analytical Chemistry, University of Pannonia, Veszprém, Hungary; 2School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Geneva, Switzerland; 3Center of Immunology Pierre Fabre, Saint-Julien-en-Genevois, France; 4Egis Pharmaceuticals Plc., Budapest, Hungary
P 11Development of new spongy-monolithic columns for selective separation of extracellular vesicles
S. KATO1, T. NAITO1, T. KUBO1, K. AKIYOSHI2, K. OTSUKA1
1Graduate School of Engineering, Department of Material Chemistry, Kyoto University, Japan; 2Graduate School of Engineering, Department of Polymer Chemistry, Kyoto University, Japan
P 12High-resolution, high-throughput characterization of protein therapeutic size variants by size exclusion chromatography
V. Qin1, A. Coffey2, J. Meixner3*
1Agilent Technologies, Wilmington, USA; 2Agilent Technologies, Church Stretton, UK; 3Agilent Technologies, Waldbronn, Germany
P 13Site-specific conjugation of metal-chelating polymers for radioimmunotherapy applications
S. Lin1, J. Baek1, B.J. Agnew2, P.J. Le3, V. Facca4, E. Lu3, M.A. Winnik3, R.M. Reilly4
1Chromatography Columns and Consumables, Thermo Fisher Scientific, Sunnyvale, CA; 2Licensing and Commercial Supply, Thermo Fisher Scientific, Eugene, USA; 3Departments of Chemistry, University of Toronto, Canada; 4Pharmaceutical Sciences, University of Toronto, Canada
P 14Development of novel reversed-phase packing material for improved separation of protein biopharmaceuticals including intact antibodies
D. Esser, K. Tsutsui, C. Matsumura, N. Shoji, H. Kanezaki, N. Kuriyama
YMC Europe GmbH, Dinslaken, Germany; YMC Co., Ltd., Kyoto, Japan
P 15Comparison of Imaged Capillary Isoelectric Focusing and Weak Cation Exchange Chromatography for Analysis of Charge Variants of Therapeutic Monoclonal Antibodies
S. Tremintin, J. Baek, S. Lin
Thermo Fisher Scientific, Sunnyvale, USA
P 16A universal mobile phase system for simple pH optimization of mobile phases used for ion exchange chromatography and hydrophobic interaction chromatography
S. Tremintin, J.M.T. Wong, J. Baek, S. Lin
Thermo Fisher Scientific, Waltham, USA
P 17Comparative analysis of fluorophore labeled N-glycans using liquid phase separation methods
C. Filep1, B. Borza1, M. Szigeti1, Z. Demianova2, A. Guttman1,2
1Horváth Csaba Laboratory of Bioseparation Sciences, Research Center for Molecular Medicine, Faculty of Medicine, University of Debrecen, Hungary; 2Sciex, Brea, USA
P 18Measurement of aggregation in high concentration biopharmaceutical formulations by microbore SEC with UV imaging detection
D. Goodall*, P. Jepson
Paraytec Ltd., York, UK
P 19ORTHOGONAL METHODS IN STABILITY TESTING OF LACTASE ENZYME, A MODEL OF BIOLOGICALS
B. Dalmadi Kiss1, M. Király1, L. Drahos2, P. Horváth3, A. Mirzahosseini3, G. Pálfy4, L. Márk5, I. Klebovich1, I. Antal1, K. Ludányi1
1Department of Pharmaceutics, Faculty of Pharmacy, Semmelweis University, Budapest, Hungary; 2MS Proteomics Research Group, Research Centre for Natural Sciences, Hungarian Academy of Sciences, Budapest, Hungary; 3Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Semmelweis University, Budapest, Hungary; 4Laboratory of Structural Chemistry and Biology, Eötvös Loránd University, Budapest, Hungary; 5Department of Biochemistry and Medical Chemistry, University of Pécs, Medical School, Hungary
P 20Creation, implementation and transferability of a Glucose Unit (GU) library for HILIC-FLD analysis of APTS-labelled released N-glycans
P. Widdowson1, A. Eckard2, J. Young2, S. Carillo3, J. Bones3, K. Salomon2
1Thermo Fisher Scientific, Runcorn, UK; 2Thermo Fisher Scientific, Oyster Point, USA; 3National Institute for Bioprocessing Research and Training (NIBRT), Dublin, Ireland
P 21Stability-indicating analytical approach for lactoferrin
R. Roškar, N. Osel, T. Planinšek Parfant, J. Trontelj, A. Kristl
University of Ljubljana, Faculty of Pharmacy, Ljubljana, Slovenia

Applications - Biomedical and clinical separations/Pharmaceutical analysis

P 22FACTORIALLY DESIGNED MIXED MICELLAR LIQUID CHROMATOGRAPHIC METHOD FOR SIMULTANEOUS DETERMINATION OF NORFLOXACIN AND TINIDAZOLE
S.F. El-Malla, A. Hassan Kamal
Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Tanta University, Egypt
P 23DEVELOPMENT OF STABILITY-INDICATING HPLC METHOD FOR QUANTIFICATION OF VANCOMYCIN HYDROCHLORIDE IN INJECTABLE PHARMACEUTICAL DOSAGE FORM
A. Serri, A. Zarghi, H. Moghimi, A. Mahboubi
Shahid beheshti university of medical sciences
P 24APPLICATION OF LC-MS/MS TO IN VITRO STUDY OF RELATIONSHIP BETWEEN SELECTED POLYPHENOLS AND TWO HUMAN CELL LINES: FETAL OSTEOBLASTIC HFOB 1.19 AND OSTEOSARCOMA SAOS-2
J. Gasik1,2, A. Strzelecka-Kiliszek2, M. Biesaga1, S. Pikuła2
1Faculty of Chemistry, University of Warsaw, Poland; 2Department of Biochemistry, Nencki Institute of Experimental Biology, Warsaw, Poland
P 25EMPLOYING MODERN LIQUID CHROMATOGRAPHY TECHNOLOGY TO SCALE A USP GRADIENT METHOD ON A SINGLE LIQUID CHROMATOGRAPHIC SYSTEM
A. Dlugasch, J. Simeone, P. McConville
Waters Corporation, Milford, Massachusetts, USA
P 26APPLICATION OF LIQUID CHROMATOGRAPHY/MASS SPECTROMETRY FOR ELECTROCHEMICAL OXIDATION OF ANTIBIOTICS IN COMPARISON TO IN VITRO AND IN VIVO METHODS
M. Szultka-Młyńska1, K. Pauter1, W. Kupczyk2, B. Buszewski1
1Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Poland;
2Department of Surgery, Collegium Medicum, Nicolaus Copernicus University, Poland
P 27BARCODE ANALYSIS BY LC/MS WITH MULTIPLE AMPLIFICATION STRATEGIES FOR CANCER BIOMARKER DETECTION
N.M. Fang, C.Y. Lee, Y.Z. Hsieh
Department of Applied Chemistry, National Chiao Tung University, Hsinchu, Taiwan, R.O.C.
P 28ANALYSIS OF VASOPRESSIN ANALOGUES IN WATER SOLUTION USING A REVERSED-PHASE TRAP COLUMN AND A HILIC ANALYTICAL COLUMN
M. Malm
Global Pharmaceutical Development, Ferring Pharmaceuticals A/S, Copenhagen, Denmark
P 29DETECTION OF ILLEGALLY ADDED PHARMACEUTICALS IN THE MEDICATIONS FROM REGISTERED CHINESE MEDICINE PRACTITIONERS
H.K. Lam
Government Laboratory, Hong Kong SAR, China
P 30HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY IN NON-CLINICAL TOXICOLOGY RESEARCH OF CONTROLLED RELEASE PHARMACEUTICAL DOSAGE FORMS IN MICROPARTICLES OF A BIODEGRADABLE ORGANIC POLYMER
S. Zhao, D. Roman, S. Benmakrelouf, Y. Danilova, D. Guérette
Charles River Laboratories, Montreal ULC (CR-MTL), Senneville, Canada
P 31UHPLC-UV COUPLED TO A SINGLE QUADRUPOLE MASS DETECTOR FOR CONFIRMATION AND QUANTITATION OF A GENOTOXIC IMPURITY IN A DRUG SAMPLE
G. Greco, S. Fabel, S. Grosse, M. De Pra, F. Steiner
Thermo Fisher Scientifc, Germering, Germany
P 32HPLC-DAD-MS STABILITY-INDICATING METHOD FOR PROPOSED FIXED-DOSE COMBINATION OF AZATHIOPRINE AND FOLIC ACID
E. Brusač, M.L. Jeličić, I. Džajić, D. Amidžić Klarić, B. Nigović, A. Mornar
Department of Pharmaceutical Analysis, Faculty of Pharmacy and Biochemistry, University of Zagreb, Croatia
P 33NOVEL RP-HPLC-DAD METHOD FOR SIMULTANEOUS DETERMINATION OF AMINOSALYCILATES AND FOLIC ACID ASSAY IN PROPOSED FIXED DOSE COMBINATIONS FOR TREATMENT OF INFLAMMATORY BOWEL DISEASE
M.L. Jeličić1, E. Brusač1, D. Amidžić Klarić1, B. Nigović1, N. Turk2, A. Mornar1
1Department of Pharmaceutical Analysis, Faculty of Pharmacy and Biochemistry, University of Zagreb, Croatia; 2Clinical Hospital Center Zagreb, Croatia
P 34AN LC-MS/MS METHOD FOR DETERMINATION OF TRIPLE DRUGS COMBINATION OF VALSARTAN, AMLODIPINE AND HYDROCHLOROTHIAZIDE IN HUMAN PLASMA FOR BIOEQUIVALENCE STUDY.
B. Arafat
Faculty of Pharmacy & Medical Sciences, Al-Ahliyya Amman University, Jordan center for pharmaceutical research, Amman, Jordan
P 35Screening of Western Drug Adulterants in Complementary Health Products by Liquid Chromatography/Mass Spectrometry/Diode Array Detector
Y.L. Quek1, W.S. Sim2, K. Chiang3, C.Y. Gan3, X. Ge1, N.P. Cheah1, M.Y. Low1
1Pharmaceutical Division, Applied Sciences Group, Health Sciences Authority, Singapore; 2Department of Chemistry, Faculty of Science, National University of Singapore, Singapore; 3Chemistry and Biological Chemistry Division, School of Physical and Mathematical Sciences, Nanyang Technological University, Singapore
P 36Predicting skin permeability of pharmaceutical and cosmetic compounds using various liquid chromatographic methods
Y. Grooten, D. Mangelings, Y. Vander Heyden
Department of Analytical Chemistry, Applied Chemometrics and Molecular Modelling, Vrije Universiteit Brussel (VUB), Belgium
P 37Charged Aerosol Detection: Factors Affecting Uniform Analyte Response
C. Paul, K. Lovejoy, F. Steiner, P. Gamache
Thermo Fisher Scientific, Germering, Germany
P 38Simultaneous determination of ribonucleoside and deoxyribonucleoside triphosphates in biological samples by hydrophilic interaction liquid
chromatography coupled with tandem mass spectrometry

Z. Kong1, S. Jia1, A.L. Chabes1, P. Appelblad2,3, R. Lundmark4,5, T. Moritz6, A. Chabes1,5
1Department of Medical Biochemistry and Biophysics, Umea University, Sweden; 2Department of Pharmacology and Clinical Neuroscience, Umea University, Sweden; 3Merck Chemicals and Life Science AB, Solna, Sweden; 4Department of Integrative Medical Biology, Umea University, Sweden; 5Laboratory for Molecular Infection Medicine Sweden (MIMS), Umea University, Sweden; 6Umea Plant Science Centre (UPSC), Department of Forest Genetics and Plant Physiology, Umea, Sweden
P 39New cell separation method using the thermoresponsive surface modified with cell adhesion peptides
M. Shimura, K. Hanaya, Y. Hiruta, K. Nagase, H. Kanazawa
Graduate School of Pharmaceutical Sciences, Keio University, Tokyo, Japan
P 40Development of a two-dimensional liquid chromatographic method for analysis of urea cycle amino acids
Y. Sumida1, K. Hamase2, T. Funatsu1, M. Tsunoda1
1Faculty of Pharmaceutical Sciences, The University of Tokyo, Japan; 2Graduate School of Pharmaceutical Sciences, Kyushu University, Fukuoka, Japan
P 41Analysis of compounds with distinct polarities in biological matrices: a case example of JAS-2
H. Bavlovič Piskáčková1, A. Reguli1, H. Jansová2, P. Brázdová3, M. Štěrba3, P. Štěrbová-Kovaříková1
1Department of Pharmaceutical Chemistry and Pharmaceutical Analysis, Faculty of Pharmacy in Hradec Králové, Charles University, Hradec Králové, Czech Republic; 2Department of Biochemical Sciences, Faculty of Pharmacy in Hradec Králové, Charles University, Hradec Králové, Czech Republic; 3Department of Pharmacology, Faculty of Medicine in Hradec Králové, Charles University, Hradec Králové, Czech Republic
P 42Advancing the peak production rate in reversed-phase gradient LC at 1500 bar to profile antibiotics samples that differ in complexity
E. Uushona1, T. Themelis1, M. De Pra2, F. Steiner2, K. Broeckhoven1, J. De Vos1, S. Eeltink1
1Vrije Universiteit Brussel, Department of Chemical Engineering, Brussels, Belgium; 2Thermo Fisher Scientific, Germering Germany
P 43A Validated HPLC-MS Method Based on Porous Graphitic Carbon Column for Determination of Twelve Thiopurine Nucleotides
D. Pecher1,2, S. Dokupilová1,2, Z. Zelinková3, J. Lučeničová4, P. Mikuš1,2
1Department of Pharmaceutical Analysis and Nuclear Pharmacy, Faculty of Pharmacy, Comenius University in Bratislava, Slovak Republic; 2Toxicological and Antidoping Center, Faculty of Pharmacy, Comenius University in Bratislava, Slovak Republic; 3Department of Gastroenterology, St Michael’s Hospital, Bratislava, Slovak Republic; 4Department of Biochemistry & Hematology, St Michael’s Hospital, Bratislava, Slovak Republic
P 44Biochromatography, a promising technique in early drug discovery
F. Tsopelas1, P. Danias1, E. Notari1, D. Anagnostopoulou1, A. Pappa1, A. Tsantili-Kakoulidou2
1Laboratory of Inorganic and Analytical Chemistry, School of Chemical Engineering, National Technical University of Athens, Greece; 2Department of Pharmaceutical Chemistry, School of Pharmacy, National and Kapodistrian University of Athens, Greece
P 45Screening therapeutics according to their uptake across the blood-brain barrier: A high throughput method based on immobilized artificial membrane liquid chromatography-diode-array-detection coupled to electrospray-time-of-flight mass spectrometry
G. Russo1, L. Grumetto2, R. Szucs3, F. Barbato2, F. Lynen4
1Separation Science Group, Department of Organic and Macromolecular Chemistry, Ghent University, Belgium; 2Department of Pharmacy, School of Medicine and Surgery, University of Naples Federico II, Italy; 3Pfizer Global R&D, Kent, United Kingdom; 4Separation Science Group, Department of Organic and Macromolecular Chemistry, Ghent University, Belgium
P 46Paradigm shift towards Method Lifecycle Management - A practical approach in establishing a SFC-based purity method for carbamazepine
A. Schmidt, M. Stanic
Chromicent GmbH, Berlin, Germany
P 47Azithromycin analysis according to latest US Pharmacopoeia monographs using HPLC-ECD
D. Friscione1, L. van Heerwaarden2, J.P. Chervet2, M. Eysberg2
1Alfatech SpA, Genova, Italy; 2Antec Scientific, Zoeterwoude, The Netherlands
P 48Determination of immune system activation markers, uric acid and creatinine in human saliva using HPLC
A. Vernerová1,2, L. Kujovská Krčmová1,2
1Department of Analytical Chemistry, Faculty of Pharmacy in Hradec Králové, Charles University, Czech Republic; 2Department of Clinical Biochemistry and Diagnostics, University Hospital Hradec Králové, Czech Republic
P 49Development and validation of two orthogonal HPLC methods for the study of the accelerated stability assessment program (ASAP) of Acetylsalicylic acid
P. Appelblad1,3, A. Speltini2, G. Marrubini2
1Department of Pharmacology, Umea University, Sweden; 2Department of Drug Sciences, University of Pavia, Italy; 3Merck Chemicals and Life Science AB, Solna, Sweden
P 50Ultra-high performance liquid chromatography in bioactivation study of an anticancer drug sobuzoxane
P. Reimerová1, A. Jirkovská2, H. Bavlovič Piskáčková1, P. Štěrbová-Kovaříková1
1Department of Pharmaceutical Chemistry and Pharmaceutical Analysis, Faculty of Pharmacy in Hradec Králové, Charles University, Akademika Heyrovského 1203, Czech Republic; 2Department of Biochemical Sciences, Faculty of Pharmacy in Hradec Králové, Charles University, Akademika Heyrovského 1203, Czech Republic
P 51UHPLC-MS/MS determination of urinary retinol as a potential new biomarker in clinical
K. Kučerová1,2, L. Kujovská Krčmová1,2, L. Matysová1
1Department of Analytical Chemistry, Faculty of Pharmacy in Hradec Králové, Charles University, Hradec Králové, Czech Republic; 2Department of Clinical Biochemistry and Diagnostics, University Hospital Hradec Králové, Czech Republic
P 52Simple and rapid measurement of encapsulation efficiency of drug in liposomes using a novel monolithic silica column with nanoparticle exclusion chromatography
K. Miyashita1, E. Yamamoto2,3, S. Miyazaki1, C. Aoyama1, I. Kushida2, T. Hasebe2, M. Kato4

1GL Sciences Inc.; 2Formulation Research Laboratories, Pharmaceutical Science and Technology Core Function Unit, Medicine Development Center, Eisai Co., Ltd.; 3National Institute of Health Sciences; 4School of Pharmacy, Showa University
P 53Rapid high-performance liquid chromatographic determination of colloidal silver–induced oxidative stress biomarker in microbially inoculated media
K.C. Hsu1, Y.C. Chen2, J.R. Hsieh1, P.F. Hsu1, Y.Y. Chao3, Y.L. Huang1,2
1Department of Medical Laboratory Science and Biotechnology, College of Health Sciences, Kaohsiung Medical University, Kaohsiung, Taiwan, R.O.C; 2Department of Laboratory Medicine, Kaohsiung Medical University Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan, R.O.C; 3Department of Public Health, College of Health Sciences, Kaohsiung Medical University, Kaohsiung, Taiwan, R.O.C
P 54Optimisation of oligonucleotide separations on ion-exchange chromatography
D. Esser, A. Matsui, S. Nozawa, N. Kuroda
YMC Europe GmbH, Dinslaken, Germany; YMC Co., Ltd., Kyoto, Japan
P 55Development of Methods for the Separation of Major Cannabinoids and Terpenes using six Column Selectivity Screening
A. McKeown1, G. Lo1, I. Phillips2, P. Bridge2
1Advanced Chromatography Technologies Ltd., Aberdeen, Scotland, UK; 2Avantor, VWR International Ltd., Lutterworth, UK
P 56Pharmaceutical Applications of Ion Chromatography-Mass Spectrometry (IC-MS)
B. Jackson1, A. Ray1, T. Corry2, Z. Lewis2
1Pharmaceutical Technology and Development, AstraZeneca, UK; 2Department of Chemistry, University of Manchester, UK
P 57Maximisation of peak capacity for peptide mapping using a column coupling approach with solid-core columns
A.P. McKeown1, P. Bridge2, I. Phillips2
1Advanced Chromatography Technologies Ltd., Aberdeen, Scotland, UK; 2Avantor, VWR International Ltd., Lutterworth, UK
P 58LC/MS/MS Analysis of Fentanyl and Related Analogs Using Biocompatible Solid Phase Microextraction
1P. Lewits; 2E. Barrey; 3C.E. Muraco
1Merck KGaA, Chromatography & Instrumental Analysis, Darmstadt, Germany; 2Supelco Inc, Sample Preparation and Workflow R&D, Bellefonte, USA; 3Supelco Inc, Liquid Separations R&D, Bellefonte, USA
P 59Identification and quantification of lipids in vaccines by HPTLC
Cotte Jean-François 1, Martial Fabien 1, Dubayle Jean 1, Talaga Philippe 2
1 Analytical Sciences, Sanofi Pasteur, Marcy l'Etoile, France
2 Global MTech, Sanofi Pasteur, Marcy l'Etoile, France
P 60Development of UHPLC method for determination of salicylic acid, methyl-salicylate and ethylsalicylate in pharmaceutical formulations
L. Matysová1, K. Kučerová1,2, N. Kosáčková1
1Department of Analytical Chemistry, Faculty of Pharmacy in Hradec Králové, Charles University, Czech Republic; 2Department of Clinical Biochemistry and Diagnostics, University Hospital Hradec Králové, Czech Republic
P 61Development and validation of two orthogonal HPLC methods for the study of the accelerated stability assessment program (ASAP) of Acetylsalicylic acid
P. Appelblad1,3, A. Speltini2, G. Marrubini2
1Department of Pharmacology, Umea University, Sweden; 2Department of Drug Sciences, University of Pavia, Italy; 3Merck Chemicals and Life Science AB, Solna, Sweden
P 62Chemiluminescent nitrogen detection – a neglected LC detection technique for determination of relative response factors in pharmaceutical analysis
J. Neumann, A.H. Schmidt, M. Stanic
Chromicent GmbH, Berlin, Germany
P 63Application of dried blood spots for quantitative analysis of biological metabolites
H.D. Cho, Y.Y. Kim, J.Y. Lee, Y. Lee, E. Jo, S. Seo, G. Kim, S.B. Han*
Department of Pharmaceutical Analysis, College of Pharmacy, Chung-Ang University, Seoul, Republic of Korea
P 64Studying organoid drug metabolism using liquid chromatography (UHPLC and nano-format) and mass spectrometry
A. Mrsa1, I.C.S. Boger1, E. Nerem2, F.S. Skottvoll1, S. Harrison3, G. Sullivan3, E. Lundanes1, I.L. Bogen2, S.R. Wilson1
1Detpartment of Chemistry, University of Oslo, Norway; 2Section for Drug Abuse Research, Department of Forensic Sciences, Division of Laboratory Medicine, Oslo University Hospital, Norway; 3Hybrid Technology Hub-Centre of Excellence, Institute for Basic Medical Sciences, Faculty of Medicine, University of Oslo, Norway
P 65Protein analysis of liver organoids with minaturized LC-MS: nano and PLOT format
A. Hermansen1,2, M. Schüller1,2, F.S. Skottvoll1,2, T. Hara3, G. Desmet4, E. Lundanes1, S.R. Wilson1,2
1Department of Chemistry, University of Oslo, Norway; 2Hybrid Technology Hub Centre of Excellence, Institute of Basic Medical Sciences, Faculty of Medicine, University of Oslo, Norway; 3Division of Metabolomics, Medical Institute of Bioregulation, Kyushu University, Fukuoka, Japan; 4Department of Chemical Engineering, Vrije Universiteit Brussel, Belgium
P 66Validated HPLC methods with fluorescence detection for the quantitation of ciprofloxacin and ampicillin antibiotics in mouse faecal samples to study bacterial multidrug resistancy
M.K. Nyírő1, J. Erdőssy1, B. Varga1, F. Balázs Farkas3, D. Szabó3, V. Horváth1,2
1Budapest University of Technology and Economics, Department of Inorganic and Analytical Chemistry, Budapest, Hungary; 2MTA-BME Computation Driven Chemistry Research Group, Budapest, Hungary; 3Semmelweis University, Institute of Medical Microbiology, Budapest, Hungary
P 67A Novel Solution for Vitamin K1 and K2 Analysis in Human Plasma by LC-MS/MS
T. Kahler, S.H. Liang, R. Alagandula, F. Carroll, P. Connolly, D. Bell
Restek Corporation, Bellefonte, USA
P 68Analysis of Phosphatidylethanol (PEth) in Human Whole Blood by LC-MS/MS
T. Kahler, S.H. Liang, R. Alagandula, F. Carroll, P. Connolly
Restek Corporation
P 69A lab-on-a-disk device for isolation and identification of parasite eggs in stool
A. Kryj1, S. Sukas1, B. Van Dorst2, O. Lagatie2, W. De Malsche1, L. Stuyver2
1Vrije Universiteit Brussel, Belgium; 2Janssen Diagnostics, Beerse, Belgium
P 70a bio-analytical case study of monoamine HPLC-ECD analysis in rat brain matrix
S. Andresen, B. Anzelius, A. Josefsson, A.S. Hansen, D. Kirik, U. Schagerlöf
Lund University, Experimental Medical Science, Lund, Sweden
P 71The investigation of hydroxy acid - lactone interconversion of statins in biological matrix. Application of hplc-ms/ms methods for clinical trials
D.A. Biianova, S.I. Kononova, A.A. Nikitina, A.V. Grigoriev, A.A. Sidorova
Bioanalytical Laboratory CSU “Analytical Spectrometry”, Saint-Petersburg, Russia
P 72Determination of ibandronic acid in human plasma by LC-MS/MS for pharmacokinetic applications
D. Iaroshenko, A. Nikitina, A. Grigoriev, A. Sidorova
CSU Analytical Spectrometry, Saint-Petersburg, Russian Federation

Applications - Development of chromatographic methods for QC

P 73METHOD DEVELOPMENT AND VALIDATION: WHERE IS ANALYTICAL CHEMIST REALLY NEEDED?
A. Laaniste, K. Herodes
University of Tartu, Institute of Chemistry, Tartu, Estonia
P 74COMPARISON OF THE PROPOSALS FOR THE ESTIMATION OF PREDICTION AND CONTENT TOLERANCE INTERVALS IN CHROMATOGRAPHIC ASSAY METHODS VALIDATION
J.M. Roussel, M. Righezza
Consultant, Macon, France; Aix-Marseille universite, Marseille, France
P 75SELECTION OF THE MOST APPROPRIATE STATIONARY PHASE FOR CHROMATOGRAPHIC DETERMINATION OF COMPONENTS OF SIMILAR POLARITY IN METHOD DEVELOPMENT
S. Zubčić1, V.G. Marković2, R. Truban-Žulj1
1Agency for Medicinal products and Medical Devices of Croatia, Zagreb, Croatia; 2University of Zagreb, Faculty of Chemical Engineering and Technology, Zagreb, Croatia
P 76NEW TRENDS ON ANALYTICAL METHOD VALIDATION: THE ANALYTICAL PROCEDURE LIFECYCLE
KIMBER BARNETT; GREG MARTIN; HORACIO PAPPA

K. Barnett, G. Martin, H. Pappa
United States Pharmacopeia, Rockville, USA
P 77ROBUSTNESS ASSESSMENT IN COMPUTER-ASSISTED LIQUID CHROMATOGRAPHY PROCEDURES BASED ON DESIRABILITY FUNCTIONS
S. Triñanes, P. Rodriguez-Mier, C. Cobas, E. Sánchez, R. Phan-Tan-Luu, R. Cela
Dpt. of Analytical Chemistry, Research Institute of Food Analysis, University of Santiago de Compostela, Spain; Centre for Information Technologies, University of Santiago de Compostela, Spain; Mestrelab Research S.L., Spain; Mestrelab Research S.L., Spain; University Paul Cezanne, Marseille, France; Dpt. of Analytical Chemistry, Research Institute of Food Analysis, University of Santiago de Compostela, Spain
P 78A SAFE AND PRECISE METHOD TO ANALYZE 4-TERT-BUTYLCATECHOL IN PRESSURIZED LIQUEFIED HYDROCARBON STREAMS USING LIQUID CHROMATOGRAPHY
A. König, M. Dunkle
Dow Benelux, Analytical Science, Terneuzen, The Netherlands
P 79QUALITY BY DESIGN IN THE DEVELOPMENT OF HYDROPHILIC INTERACTION LIQUID CHROMATOGRAPHY METHOD WITH GRADIENT ELUTION FOR THE ANALYSIS OF OLANZAPINE
A. Tumpa1, J. Maksić Janković2, G. Forsdahl3, B. Jančić-Stojanović1
1Department of Drug Analysis, Faculty of Pharmacy, University of Belgrade, Serbia; 2Department of Drug Control and Examination, Service for Pharmaceutical Activity, Military Medical Academy, Belgrade, Serbia; 3Department of Pharmacy, University of Tromsø, Norway
P 80STREAMLINED METHOD DEVELOPMENT FOR ACTIVE PHARMACEUTICAL INGREDIENTS IN COLD AND FLU MEDICATION USING A SYSTEMATIC PROTOCOL
M. Maziarz, P. Rainville
Waters Corporation, Milford, USA
P 81ANALYSIS OF ATROPISOMERS OF API’S BY HPLC AND SFC
A. Hessler1, E. Zafrir Ilan1, T. Ho1, M. Stanic2, A. Schmidt2
1Gilead Sciences Inc., Foster City (CA); 2Chromicent GmbH, Berlin (DE)
P 82ANALYTICAL STRATEGIES TO QUANTITATE VOLATILE DEGRADANTS IN DRUG SUBSTANCE
H. Marshall, Y. Gao
Gilead Sciences Inc., Foster City (CA)
P 83DOE APPROACH TO EFFICIENT ASSAY AND DEGRADATION PRODUCT METHOD DEVELOPMENT FOR FIXED DOSE COMBINATION TABLETS
T. Waki, C.I. Alvarado, N. Gumataotao, C. Chan, V. Rucker
Gilead Sciences Inc., Foster City (CA)
P 84CHARGED AEROSOL DETECTION AND METHOD TRANSFER OF COMPENDIAL, INCLUDING USP, METHODS
M. Pietsch, K. Lovejoy, I. Acworth, P. Gamache
Thermo Fisher Scientific, Dreieich, Germany; Thermo Fisher Scientific, Germering, Germany; Thermo Fisher Scientific, Chelmsford, USA; Thermo Fisher Scientific, Chelmsford, USA
P 85Modernization of USP Monographs for Naphazoline Hydrochloride and Pheniramine Maleate Ophthalmic and Nasal Solutions
M. Maziarz, S. Ramakrishna, P. Rainville, S. Naughton
Waters Corporation, Milford, USA; U.S. Pharmacopeial Convention, Rockville, USA
P 86Online SEC-MR-NMR – A Powerful Tool in Polymer Analysis, with a Direct Correlation between Molar Mass Distribution (MMD) and Chemical Composition (CC)
C. Botha, J. Höpfner, M. Pollard, M. Wilhelm
Karlsruhe Institute of Technology, Chemistry and Biosciences, Institute for Technical Chemistry and Polymer Chemistry, Karlsruhe, Germany
P 87Multivariable modeling of ternary linear gradient parameters for method optimization based on precise prediction of retention behavior
T. Kawabe, M. Ono, M. Ohara
Analytical & Quality Evaluation Research Laboratories, Daiichi Sankyo Co., Tokyo, Japan
P 88System Performance Check for using Gas Chromatography with Flame Ionization Detection (GC-FID) in Analysis of C8-C40 Saturated Hydrocarbon Compounds
J.J. Seo1, H.Y. Kim1, G.S. Hwang2
1Korea Basic Science Institute, Scientific Instrument Reliability Assessment Center, Daejeon, Republic of Korea; 2Korea Basic Science Institute, Western Seoul Center, Seoul, Republic of Korea
P 89Online SEC-IR(QCL-EC) – Coupling of SEC with mid-IR Quantum Cascade Lasers for Specific Functional Group Detection in Analysis of Polymers and Copolymers
M. Pollard, C. Botha, J. Kübel, M. Wilhelm
Institute for Chemical Technology and Polymer Chemistry, Polymeric Materials Research Group, K.I.T., Karlsruhe, Germany
P 90Application of the Lifecycle Management to the analytical chromatographic methods used for the Quality Control of Itraconazole drug products
R. Klinkenberg, A. Brouwers, C. Köttgen, B. Mertens, J. Harzimont, P. Stiernon, B. Streel
Galephar M/F, Marche en Famenne, Belgium
P 91Optimization and Validation of Online Column-Switching assisted HPLC-Spectrometric Method for Quantification of Dansylated Endocannabinoids and Neurotransmitters in Animal Models of Brain Diseases
M. Baranyi, B. Sperlágh
Department of Pharmacology, Institute of Experimental Medicine, Hungarian Academy of Sciences, Budapest, Hungary
P 92DoE as an effective and comprehensive tool for RPLC method robustness evaluation
L. Volta e Sousa, A.F. Ferreira
Hovione Farmaciência S.A., R&D Analytical Development, Estrada do Paço do Lumiar, Campus do Lumiar, Lisboa, Portugal
P 93Chromatographic Support for Artemether Formulation Development: Hypercarb-facilitated, sample solvent directed assay and related substances assay
W.J. Lough, N. Khan, N. Mohd, O. Bamgbala, X. Lin, J. Ong, I. Ghulam, D. Small
School of Pharmacy and Pharmaceutical Sciences, University of Sunderland, United Kingdom

Applications - Electrically-driven separations

P 94ELECTROMEMBRANE EXTRACTION BASED ON AGAROSE GEL: AN EFFICIENT METHOD FOR EXTRACTION AND DETERMINATION OF PHENOLIC ACIDS
S. Asadi, S. Nojavan, M. Behpour
Department of analytical chemistry and pollutants, Shahid Beheshti University, Tehran, Iran
P 95DEVELOPMENT OF GLASS-BASED MICROCHIPS FOR ELECTROCHROMATOGRAPHIC SEPARATIONS
A. Kryj1, M. Baca1, E. Naghdi1,2, P. Gelin1, S. Sukas1, W. De Malsche1
1µFlow group, Department of Bioengineering Sciences, Vrije Universiteit Brussel, Belgium; 2Department of Chemistry, Shahid Beheshti University, Teheran, Iran
P 96EVALUATION OF ANTI-MIRNA OLIGONUCLEOTIDES USING CAPILLARY ELECTROPHORESIS WITH LASER-INDUCED FLUORESCENCE (CE-LIF)
E. Ban1,2, D.K. Chae1, A. Kim2, E.J. Song1
1Molecular Recognition Research Center, Korea Institute of Science and Technology, Seoul, South Korea; 2College of Pharmacy, CHA University, Seongnam, South Korea
P 97Affinity Capillary Electrophoresis of Human Serum Albumin and Small Molecules
A Comparison between Mobility Shift and Frontal Analysis

M.O. Stein1, H. Nevídalová2, R. Ratih1, Z. Glatz2, H. Wätzig1
1Institute of Medicinal and Pharmaceutical Chemistry, TU Braunschweig, Germany; 2Department of Biochemistry, Faculty of Science, Masaryk University, Brno, Czech Republic
P 98Effects of the tunable EOF on the resolution and analysis time in CE separation of helquats
V. Šolínová, D. Koval
Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences, Czech Republic
P 99Field-amplified sample injection and sweeping micellar electrokinetic chromatography in analysis of glyphosate and aminomethylphosphonic acid in wheat
R. Gotti1, J. Fiori2, S. Bosi3, G. Dinelli3
1Department of Pharmacy and Biotechnology, University of Bologna, Italy; 2Department of Chemistry “G. Ciamician”, University of Bologna, Italy; 3Department of Agricultural and Food Sciences, University of Bologna, Italy
P 100Separation of perfluorinated surfactants by non-aqueous capillary electrophoresis coupled with contactless conductivity detector
W. Grochocki, M. Breadmore
Australian Centre for Research on Separation Science, School of Natural Science, University of Tasmania, Hobart, Australia
P 101Comparison of electrodriven focusing methods for improvement of two-dimensional separations
J. Váňová, J. Sadílková, P. Česla
Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Czech Republic
P 102Separation of histones from cancer cell extracts by capillary electrophoresis with UV absorbance detection
Y.J. Lai, Z.X. Lu, P.L. Chang
Department of Chemistry, Tunghai University, Taichung, Taiwan
P 103Simplify modification mapping at the intact protein level with on-line separation and analysis by CE-MS
S. Lock1, W. Belloni2
1Sciex, Warrington, UK; 2Sciex, Milan, Italy

Applications - Environmental analysis

P 104HILIC – EFFECT OF SALT-CONTAINING AQUEOUS MATRICES
M.J.C. Brekelmans, C.W.S. Goossens, E.M. Reingruber, K. Scase
Charles River laboratories,'s-Hertogenbosch, the Netherlands
P 105IDENTIFICATION OF BISPHENOL A IN BOTTLED WATER USING SOLID-PHASE EXTRACTION AND ULTRA-PERFORMANCE LIQUID CHROMATOGRAPHY–TANDEM MASS SPECTROMETRY
A.M. Alammari1, M. Rizwan Khan1, A. Aqel1, Z.A. Alothman1, I.H. Alsohaimi2, A. Alsubhi1
1Department of Chemistry, College of Science, King Saud University, Riyadh, Saudi Arabia; 2Chemistry Department, College of Science, Jouf University, Sakaka, Saudi Arabia
P 106A newly developed procedure for quantifying leaf pigments of broadleaf trees
F. Petibon, M.W.I. Schmidt, G.L.B. Wiesenberg
Soil science and biogeochemistry, Department of Geography, University of Zurich, Switzerland
P 107ANALYTICAL PLATFORM FOR NON-TARGETED FINGERPRINTING ANALYSIS OF METAL(LOID)-CONTAINING ORGANIC COMPOUNDS IN ENVIRONMENTAL SAMPLES
P. Denti, H.R. Hansen, L.M.V. Malmquist, D.B.A. Malheiro, G. Tomasi, J.H. Christensen
1,3,5,6Department of Plant and Environmental Sciences, University of Copenhagen, Frederiksberg; 2Danish Environmental Protection Agency, Odense, Denmark; 4MS-Omics, Vedbaek, Denmark
P 108POLYCYCLIC AROMATIC HYDROCARBONS (PAHS) IN THE RED MULLET (MULLUS BARBATUS) USING QUECHERS EXTRACTION AND UHPLC-FLUORESCENCE DETECTION
E. Frapiccini1, M. Panfili1, S. Guicciardi1, A. Santojanni1, M. Mauro1, M. Betti1, C. Truzzi2, M. Antonucci2, F. Girolametti2, S. Illuminati2, G. Scarponi2, A. Annibaldi2
1National Research Council, CNR-IRBIM, Ancona, Italy; 2Department of Life and Environmental Sciences, Università Politecnica delle Marche, Ancona, Italy
P 109RAPID DETERMINATION OF THE GLYPHOSATE IN SOIL BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
K. Ashimuly, Z. Tukenova, U. Alimov, M. Alimzhanova
Department Natural Science Disciplines, Kazakhstan Engineering and Technological University, Almaty, Kazakhstan; Department of Chemistry and Chemical Technology, Al-Farabi Kazakh National University, Almaty, Kazakhstan
P 110Optimization of SPE extraction and HPLC separation for monitoring of pharmaceuticals in water
P. Šilarová1, N. Roulová2, L. Česlová3, D. Šilha4
1Department of Inorganic Technology, University of Pardubice, Czechia; 2Department of Biological and Biochemical Science, University of Pardubice, Czechia; 3Department of Analytical Chemistry, University of Pardubice, Czechia; 4Department of Biological and Biochemical Science, University of Pardubice, Czechia
P 111Instability and Complexity of Polycyclic Musk Galaxolide
D.M. Makey1, A. Crawford1, D.C. Harmes1, K.H. Wammer2, D.R. Stoll1
1Gustavus Adolphus College, Saint Peter, Minnesota, USA; 2University of Saint Thomas, Saint Paul, Minnesota, USA
P 112Trace analysis of haloacetic acids in environmental water samples using high-pressure ion chromatography-mass spectrometry
J. Boertjes, E. Gilchrist
School of Chemistry, University College Cork, Ireland; a School of Chemistry, University College Cork, Ireland, b Environmental Research Institute, University College Cork, Ireland
P 113DETERMINATION OF POLYCYCLIC AROMATIC HYDROCARBONS FROM SOIL BY USING HPLC
I. Kiss
Department of Analytical and Environmental Chemistry, University of Pécs, Hungary
P 114Determination of haloacetic acids formed in model systems by liquid chromatography-tandem mass spectrometry
A. Bednáriková1, K. Munka2, J. Ilavský3, D. Barloková3, S. Varga2, M. Slovinská2, A. Vajíčeková2, K. Jančurová2
1Water Research Institute, National Water Reference Laboratory, Bratislava, Slovakia; 2Water Research Institute, Division of Water Quality, Bratislava, Slovakia; 3Slovak University of Technology in Bratislava - Faculty of Civil Engineering, Department of Sanitary and Environmental Engineering, Bratislava, Slovakia
P 115Development of a multiclass method for pharmaceuticals determination in water applying rotating-disk sorptive microextraction coupled offline to UHPLC-MS/MS
W. Calzadilla1, C.G. Peña1, R. Salazar2, M. Aranda1
1University of Concepcion, Bio Bio Region. Chile; 2University of Santiago, Metropolitan Region. Chile
P 116Molecular-level Investigation of soils contaminated oil from Gulf War after 30 Years using FT-ICR MS and GCxGC/HRMS
M. Park1, E. Cho2, M. Hur3, G. Kang4, S. Kim2, Y.H. Kim1
1Biomedical Omics Group, Korea Basic Science Institute, Cheongju, Republic of Korea; 2Dept. of Chemistry, Kyungpook National University, Daegu, Republic of Korea; 3Dept. of Botany and Plant Sciences, and Institute of Integrative Genome Biology, University of California, Riverside, USA; 4Dept. of Environmental Sciences, Hankuk University of Foreign Studies, Yongin, Republic of Korea
P 117Emerging contaminant occurrence in a London urban river system measured using passive sampling and liquid chromatography-high resolution accurate mass spectrometry
A.K. Richardson, A. Gavell, G.R. Fones, G.A. Mills, A.M. Edge, D.A. Cowan, D.J. Neep, L.P. Barron
School of Population Health & Environmental Sciences, King's College London, United Kingdom; Natural Resources Wales, Llanelli, United Kingdom; School of Pharmacy and Biomedical Sciences, University of Portsmouth, United Kingdom; Agilent Technologies LDA UK Ltd., Church Stretton, United Kingdom
P 118Development of an innovative method to determine glyphosate, AMPA and glufosinate in fresh water using on-line SPE combined with HPAEC-MS
R. Zangrando1, E. Barbaro1, M. Feltracco2, C. Barbante1,2, Andrea Gambaro1,2
1Institute for the Dynamics of Environmental Processes CNR, Mestre, Italy; 2Department of Environmental Sciences, Informatics and Statistics, Ca’ Foscari University of Venice, Italy
P 119Simultaneous determination of phthalate metabolites in DBS (dried blood spot) using liquid chromatography with tandem mass spectrometry (LC-MS/MS)
J.Y. Lee, H.D. Cho, E. Jo, Y. Lee, Y.Y. Kim, S. Sumin, K. Gunwoo, S.B. Han
Department of Pharmaceutical Analysis, College of Pharmacy, Chung-Ang University, Seoul, Republic of Korea
P 120Origin of life: Experimental evolution of functional vesicles in hydrothermal environments
Y. Danisan1,2, M. Sulkowski1,2, U. Schreiber3, C. Mayer4, O.J. Schmitz1,2
1Institute of Applied Analytical Chemistry (AAC), University of Duisburg-Essen, Germany; 2Teaching and Research Centre for Separation (TRC), Essen, Germany; 3Department of Geology, University of Duisburg-Essen, Germany; 4Institute of Physical Chemistry, CENIDE, University of Duisburg-Essen, Germany
P 121Simultaneous determination of phthalate metabolites in dried blood spot using liquid chromatography with tandem mass spectrometry
J.Y. Lee, H.D. Cho, E. Jo, Y. Lee, Y.Y. Kim, S. Sumin, K. Gunwoo, S.B. Han
Department of Pharmaceutical Analysis, College of Pharmacy, Chung-Ang University, Seoul, Republic of Korea
P 122Impact of large historical eruptions on fluoride records obtained from Antarctic ice cores using capillary ion chromatography
E. Sanz Rodriguez1, C. Plummer2, M. Nation3, A. Moy2,3, M. Curran2,3, P.R. Haddad1, B. Paull1
1Australian Centre for Research on Separation Science, School of Natural Sciences, University of Tasmania, Australia; 2Antarctic Climate and Ecosystems Cooperative Research Centre, University of Tasmania, Australia; 3Australian Antarctic Division, Tasmania, Australia
P 123Analysis of Bisphenols and Perfluorinated Compounds by LC-MS/MS Using Raptor 1.8 µm Columns
P. Connolly, L.A. Wiest, S.H. Liang, J. Konschnik, T. Kahler
Restek Corporation, Bellefonte, USA
P 124Direct analysis of illicit and pharmaceutical substances in wastewater using rapid LC-MS/MS
K.T. Ng1, H. Rapp-Wright1, L. Couchman2, Leon Barron1
1Dept. Analytical, Environmental & Forensic Sciences, King's College London, UK; 2Toxicology Unit, Department of Clinical Biochemistry, King's College Hospital NHD Foundation Trust, London, UK
P 125Ion Chromatography Determination of Nitrite, Nitrate, and Bromide in Seawater by UV/Vis Detection after Suppression
P. Jochems, V. Kraft, G. Schad, R. Ludwig
Shimadzu Europa GmbH, Duisburg, Germany
P 126Simultaneous enrichment and hplc-ms/ms quantification of several classes of algal toxins in environmental waters
F. Merlo1, D. Piparo1, F. Maraschi1, A. Speltini2, A. Profumo1
1Department of Chemistry, University of Pavia, Italy; 2Department of Drug Sciences, University of Pavia, Italy
P 127SPECIATION ANALYSIS OF ORGANOTIN COMPOUNDS IN ENVIRONMENTAL SAMPLES
G. Vergine, E. Sarti, V. Costa, A. Cavazzini, L. Pasti
Università degli Studi di Ferrara, Italy
P 128Determination of blood plasma testosterone and corticosterone in barn swallow (Hirundo rustica)
Z. Bílková1, M. Kotasová Adámková1,2, O. Kauzál3, Z. Šimek1, T. Albrecht3
1Research Centre for Toxic Compounds in the Environment, Masaryk University, Brno, Czech Republic; 2Department of Botany and Zoology, Masaryk University, Brno, Czech Republic; 3Institute of Vertebrate Biology, Academy of Sciences, Studenec, Czech Republic; 4Department of Zoology, Charles University, Prague, Czech Republic

Applications - Food analysis

P 129A SINGLE-STEP SOLID-PHASE EXTRACTION FOR DETERMINATION OF PATULIN IN APPLE JUICE BY LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY
X. Li, H. Li
National Institute of Metrology
P 130ESTABLISHMENT OF A RAPID ANALYTICAL METHOD FOR SIMUTANEOUS DETERMINATION OF THE EU PRIORITY POLYCYCLIC AROMATIC HYDROCARBONS (PAHS) IN CHAR-GRILLED CHICKEN DRUMSTICKS
D.J. Yang1,2, C.F. Chiang2,3, K.C. Hsu2,4, C.Y. Cho2, T.Y. Tsai2, C.H. Hsu1,2
1Institute of Food Safety and Health Risk Assessment, National Yang-Ming University, Taipei, Taiwan; 2Master Program of Food and Drug Safety, China Medical University, Taichung, Taiwan; 3Department of Health Risk Management, China Medical University, Taichung, Taiwan; 4Department of Nutrition, China Medical University, Taichung, Taiwan
P 131DETERMINATION OF POLYMER ADDITIVES IN FOODS AND DRINKS PACKED WITH PLASTIC BY MAGNETIC SOLID-PHASE EXTRACTION OF AMINO GROUP MODIFIED MAGNETIC MESOPOROUSE SILICA MICROSPHERES COUPLED WITH HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
X. Liu, Z. Guo, X. Zhao, Q. Li, Y. Wei
State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing, China
P 132LACTOSE CONTENT IN ‘LACTOSE-FREE’ LABELLED DAIRY PRODUCTS (LACTOSE INTOLERANCE)
J.P. Chervet1, H.J. Brouwer1, M. Eysberg1, D. Friscione2
1Antec Scientific, Zoeterwoude, The Netherlands; 2Alfatech SpA, Genova, Italy
P 133EFFECTS OF HARVEST TIME ON PHENOLIC COMPOSITIONS AND ANTIOXIDANT ATTRIBUTES OF LEAVES OF EIGHT PERSIMMON VARIETIES
P.J. Wu1,2, Y.L. Chang3,4, D.J. Yang1, J.T. Li5, H.L. Li4
1Institute of Food Safety and Health Risk Assessment, National Yang-Ming University, Taipei, Taiwan; 2Dikma Technologies Inc., Markham, Canada; 3Miaoli District Agricultural Research and Extension Station, Council of Agricultural, Miaoli, Taiwan; 4Department of Horticulture, National Chung Hsing University, Taiwan; 5Department of Medical Applied Chemistry, Chung Shan Medical University, Taichung, Taiwan
P 134MOLECULAR IMPRINTED POLYMER@MAGNETIC NANOPARTICLES COMBINED WITH LIQUID CHROMATOGRAPHY MASS SPECTROMETRY FOR DETERMINATION OF GLYCOALKALOIDS IN POTATO
C.H. Yeh, C.Y. Chen, M.R. Lee
Department of Chemistry, National Chung Hsing University, Taichung, Taiwan
P 135CHEMICAL PROFILE OF MORUS NIGRA EXTRACT OBTAINED BY PRESSURIZED HOT WATER EXTRACTION
N. Nastić1, A. Segura-Carretero2,3, J. Švarc-Gajić1, I.l Borrás-Linares3, J. Lozano-Sánchez3
1Faculty of Technology, University of Novi Sad, Novi Sad, Serbia; 2Department of Analytical Chemistry, Faculty of Science, Granada, Spain; 3Functional Food Research and Development Centre (CIDAF), Health Science Technological Park, Granada, Spain
P 136A RAPID ULTRASOUND-ASSISTED DISPERSIVE LIQUID-LIQUID MICROEXTRACTION FOLLOWED BY LIQUID CHROMATOGRAPHY COUPLED TO A MASS SPECTROMETRY FOR DETERMINATION OF ARTEMISININ IN TILAPIA MUSCLE
M.P. Soares1, L.S. Shiroma2, I.L. Cardoso1, F.G. Sampaio3, C.M. Jonsson3, F.T. Rantin1, S.C.N. Queiroz3
1Department of Physiological Sciences, Federal University of São Carlos, Brazil; 2Institute of Chemistry, UNICAMP, Department of Analytical Chemistry, Campinas, Brazil; 3Embrapa Environment, Brazilian Agricultural Research Corporation (EMBRAPA), Jaguariúna, Brazil
P 137A NEW GASTRO-INTESTINAL SYSTEM TO EVALUATE THE EFFECT OF FOOD METHYLGLYOXAL
L. Ferron1,2, R. Colombo1, M. Bordiga3, C. Garino3, M. Paolillo1, A. Papetti1,4
1University of Pavia, Department of Drug Sciences, Pavia Italy; 2FlaNat Research Italia Srl, Rho (MI) Italy; 3University of Eastern Piedmont, Department of Pharmaceutical Sciences, Novara, Italy; 4euFAN Srl, University of Pavia, Italy
P 138HPLC-UV FINGERPRINTING TO ADDRESS NUT AUTHENTICATION BY CHEMOMETRIC METHODS
G. Campmajó1, R. Saez-Vigo1, G.J. Navarro1, N. Núñez1, J. Saurina1,2, L. Puignou1,2, O. Núñez1,2,3
1Department of Chemical Engineering and Analytical Chemistry, University of Barcelona, Spain; 2Research Institute in Food Nutrition and Food Safety, University of Barcelona, Santa Coloma de Gramanet, Barcelona, Spain;
3Serra Húnter Program, Generalitat de Catalunya, Barcelona, Spain
P 139AN EMPIRICAL APPROACH TO PREDICT SEPARATION OF WATER-SOLUBLE VITAMINS USING REVERSED-PHASE CHROMATOGRAPHY
S. Marten, S. Grosse, S.H. Park, M. De Pra, F. Steiner
Thermo Fisher Scientific, Germering, Germany
P 140ANALYSIS OF CHERRY ALLERGENS BY LIQUID CHROMATOGRAPHY/TANDEM MASS SPECTROMETRY USING STABLE ISOTOPE-LABELLED PEPTIDES
K. Ippoushi, Y. Tanaka, M. Wakagi, N. Hashimoto, Y. Takano-Ishikawa
Food Research Institute, National Agriculture and Food Research Organization, Tsukuba, Japan
P 141AUTOMATED METHOD DEVELOPMENT IN HPLC FOR THE QUANTITATIVE DETERMINATION OF CATECHINS IN TEA
S. Marten, S. Grosse, S.H. Park, M. De Pra, F. Steiner
Thermo Fisher Scientific, Germering, Germany
P 142ANALYSIS OF ACTIVE CANNABIS COMPOUNDS IN EDIBLE FOOD PRODUCTS WITH A HPLC-UV METHOD
M. Ye, K.K. Stenerson, O.I. Shimelis
MilliporeSigma, Bellefonte, USA
P 143QSight LC-MS/MS as a screening tool to detect traces of milk and egg allergens in incurred food
L. Monaci1, E. De Angelis1, R. Pilolli1, A. Ganci2
1Institute of Sciences of Food Production - Italian National Research Council (ISPA-CNR), Bari, Italy; 2Perkin Elmer Italia S.p.A., Milan, Italy
P 144Quantification of pesticide residues in fatty food matrixes: strategy of sample preparation HPLC-MS/MS analysis
S. BELARBI1,2, M. VIVIER2, P. CARDINAEL1, V. AGASSE1
1Normandie Univ, Laboratoire SMS-EA3233, UNIROUEN, Mont-Saint-Aignan Cedex, France; 2SGS France laboratoire de Rouen, Technopôle du Madrillet, Saint Etienne du Rouvray, France
P 145Toolbox for studying the chemistry of light induced degradation (TOOCOLD): towards an online degradation system
M. den Uijl, B. Pirok, P. Schoenmakers, M. van Bommel
Van 't Hoff Institute for Molecular Sciences, University of Amsterdam, The Netherlands
P 146Determination of cholecalciferol in milk and dairy product samples by using dispersive liquid-liquid microextraction method combined with liquid chromatography
T. Hájek, V. Hladíková
Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Czech Republic
P 147Authentication of Garcinia cambogia supplements by HPLC-UV-MS
A. Mena1, A.J. Belleizac2, D.M. Chito2, M.P. Rada-Mendoza2, A.I. Ruiz-Matute1, A.C. Soria1, M.L. Sanz1
1Instituto de Química Orgánica General (IQOG-CSIC), Madrid, Spain; 2Departamento de Química, Facultad de Ciencias Naturales,Exactas y de la Educación, Universidad del Cauca, Popayán, Colombia
P 148Profiling of phenolic flavourings in whiskey by reversed phase liquid chromatography with electrochemical detection at a boron doped diamond electrode
P.E. Hayes1,2, A. Buzid1,2, J.H. T. Luong1,2, E.S. Gilchrist1,2, J.D. Glennon1,2
1Innovative Chromatography Group, Irish Separation Science Cluster (ISSC), University College Cork, Ireland; 2School of Chemistry and the Analytical, Biological and Chemistry Research Facility (ABCRF), University College Cork, Ireland
P 149Native amino acid analysis in wine by HILIC separation and detection with single quadrupole mass spectrometry
S.H. Park, H. Herrmann, K. Lovejoy, S. Grosse, M. De Pra, S. Meding, M. Menz, F. Steiner
ThermoFisher Scientific, Chromatography and Mass Spectrometry Division, Dreieich, Germany; Thermo Fisher Scientific, Chromatography and Mass Spectrometry Division, Germering, Germany
P 150The Advantages of TLC as a Quick Screening and Crosscheck Method for Natural Products using the Quantification of α- and β- Acids in Hop as an Example
J. Engemann1, V. Pilakowski1, M. Oberle1, M. Burholt1, M. Schulz1, M. Bäumle2*




1Merck KGaA, Life Science, Darmstadt, Germany; 2Sigma-Aldrich Chemie GmbH, Switzerland (Sigma-Aldrich Chemie GmbH is a subsidiary of Merck)
P 151Sulfur speciation analysis in wine by HPLC-ICPMS/MS
B. Lajin, W. Goessler
Institute of Chemistry – Analytical Chemistry for Health and Environment (ACHE), University of Graz, Austria
P 152Chlorogenic acids and caffeine in green and roasted coffee by HPLC-DAD
A. Schmidt, L. Macheiner, S. van den Oever, H.K. Mayer
Department of Food Science and Technology, Food Chemistry Laboratory, BOKU - University of Natural Resources and Life Sciences Vienna, Austria
P 153Improved Fermentation Process Monitoring using Dual Injection UHPLC System
T. Yamaguchi, K. Koterasawa, H. Terada,K. Matsumoto
Shimadzu Corporation Analytical & Measuring Department, Kyoto, Japan
P 154Determination of Naturally occurring benzoic acid and sorbic acid in vegetable, animal and their processed foods
S.H. Kim, S. Lee, J.S. So, M.Y. Lee, J.H. Lee, H.S. Lim, Y.S. Kang
Food additives and Packaging Division, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety, Cheongju, Republic of Korea
P 155Determination of Chloramphenicol by QuEChERS and HPLC-MS/MS combination in Twenty Two Different Matrices
T. Śniegocki, B. Sell, M. Giergiel, A. Posyniak
Department of Pharmacology and Toxicology, National Veterinary Research Institute, Pulawy, Poland
P 156Non-targeted screening and identification of unknown compound in food supplement using LC/QTOF
S. Kotucova, L. Lakosilova, R. Stepan, M. Kubik, P. Cuhra
Czech Agriculture and Food Inspection Authority (CAFIA), Prague, CZECH REPUBLIC
P 157Oligosaccharides determination in Trentino honey by High-Performance Anionic-Exchange Liquid Chromatography coupled with Mass Spectrometry (HPAEC-MS)
R. Tedesco1,3, E. Barbaro2, G. Capodaglio1, A. Rizzoli3, P. Fontana3, V. Malagnini3, A. Gambaro1
1Department of Environmental Sciences, Informatics and Statistics, University of Venice, Ca' Foscari, Venice-Mestre, Italy; 2Institute for the Dynamics of Environmental Processes, CNR, Venice-Mestre, Italy; 3Fondazione Edmund Mach (FEM)-Centro Trasferimento Tecnologico, Trento, Italy
P 158Simple U-HPLC-MS method for evaluation of sweeteners, preservatives and purine alkaloids in soft drinks
A. Krmela, V. Schulzova, J. Pulkrabova, J. Hajslova
University of Chemistry and Technology, Department of Food Analysis and Nutrition, Prague, Czech Republic
P 159Comparison of commercial and natural black and red currant juices by LCxLC-DAD-qTOF-MS coupling mix-mode separation with reversed phase mode
L. Montero1,2, S.W. Meckelmann1,2, K. Hellmann1,2, O.J. Schmitz1,2
1University of Duisburg-Essen, Applied Analytical Chemistry, Essen, Germany; 2Teaching and Research Center for Separation (TRC), University of Duisburg-Essen, Germany
P 160Effective clean-up of 7 regulated mycotoxins in grain and subsequent determination by HPLC-MS/MS
T. Gersthagen, H.R. Wollseifen, T. Kretschmer
MACHERY-NAGEL GmbH & Co. KG, R&D Chromatography, Dueren, Germany
P 161Fast and simple method for the determination of tranquillizers in kidney by liquid chromatography with tandem mass spectrometry
B. Sell, T. Śniegocki, M. Giergiel, A. Posyniak
Department of Pharmacology and Toxicology, National Veterinary Research Institute, Pulawy, Poland
P 162Determination of cannabidiol and additional cannabinoid content in hemp tea
G. Schad, V. Kraft, P. Jochems, R. Ludwig, U. Oppermann
Shimadzu Europa GmbH, Analytical Business Unit, Duisburg, Germany
P 163A Perfect Pairing: HILIC and Superficially Porous Particles in Food Analyses
P. Connolly, L. Wiest, D. Li, J. Konschnik, T. Kahler
Restek Corporation, Bellefonte, USA
P 164Optimizing a 190+ Pesticides Multi-Residue Screening Workflow for the Preparation and Analysis of Produce by LC-MS/MS
P. Connolly, L.A. Wiest, D. Li, A.M. Pavkovich, J. Konschnik, T. Kahler, D. Bell
Restek Corporation, Bellefonte, USA
P 165Quantitation of Mycotoxins in Four Food Matrices Comparing Stable Isotope Dilution Assay (SIDA) with Matrix Matched Calibration Methods by LC-MS/MS
T. Kahler, D. Li, J. Steimling, J. Konschnik, P. Connolly
Restek Corporation
P 166Functional evaluation of fresh (FG) and black (BG) Chiloe’s giant garlic (Allium ampeloprasum L.) by High-Performance Thin-Layer Chromatography-Bioassay-Mass Spectrometry
D. Peterssen-Fonseca, J. Carrasco, K. Henríquez-Aedo, M. Aranda
Laboratory of Advanced Research on Foods and Drugs, Department of Food Science and Technology, Faculty of Pharmacy, University of Concepcion, Barrio Universitario s/n, Concepcion, Chile

Applications - Forensic, doping and toxicology

P 167HOW SAFE IS YOUR GARDEN?
S. Bowerbank, J. Dean, M. Carlin
Department of Applied Sciences, Northumbria University, Newcastle upon Tyne, UK
P 168ANALYSIS OF MICROCYSTINS LR, YR, AND RR IN BIOLOGICAL FLUIDS BY 2D-LC TECHNOLOGY
B. Garcia-Barboza1, C.R. Mallet2, S.R. Botch-Jones1
1Boston University School of Medicine, Boston, USA; 2Waters Corporation, Milford, USA
P 169SINGLE-INJECTION SCREENING OF 664 FORENSIC TOXICOLOGY COMPOUNDS USING AN INNOVATIVE BENCHTOP HIGH RESOLUTION MASS SPECTROMETER
B. Nieland3, O.G. Cabrices1, A. Wang1, X. He1, H. McCall1, L. Baker1, A. Taylor2
1SCIEX, Redwood City, USA; 2SCIEX, Concord, Ontario, Canada; 3SCIEX, Nieuwerkerk a/d IJssel, Netherlands
P 170COMPARISON OF THE KINETICS OF DYES DEGRADATION OF HANDWRITTEN STROKES SUBJECTED TO DIFFERENT TYPES OF ARTIFICIAL AGING AND STUDIED USING CHROMATOGRAPHY MASS-SPECTROMETRY AND STATISTICAL DATA PROCESSING
D. Baygildieva, A. Sherstneva, I. Plyushchenko, I. Ananieva, O. Shpigun, I. Rodin
Chemistry Department, Lomonosov Moscow State University, Moscow, Russia
P 171Gas chromatographic fingerprint analysis for the comparison of seized cannnabis samples
A. Slosse1,2, F. Van Durme2, N. Samyn2, D. Mangelings1, Y. Vander Heyden1
1Department of Analytical Chemistry, Applied Chemometrics and Molecular Modelling, Vrije Universiteit Brussel (VUB), Belgium; 2Department Drugs and Toxicology, National Institute for Criminalistics and Criminology (NICC), Brussels, Belgium
P 172Development of a rapid LC-MS/MS methodology for the analysis of Novel Psychoactive Substances (NPS) - regioisomeric phenidine derivatives
J.K. Field1, C. Titman2, C. Hinz2, M.R. Euerby1,2, O.B. Sutcliffe3
1Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, Glasgow, UK; 2Shimadzu UK Limited, Mill Court, UK; 3Manchester DRug Analysis and Knowledge Exchange (MANDRAKE), School of Science and the Environment, Faculty of Science and Engineering, Manchester Metropolitan University, John Dalton Building, UK
P 173Determination of methadone, citalopram and clomipramine and its metabolites in post mortem skeletal tissue
M. Vandenbosch1*, L. Rooseleers1, W. Van Den Bogaert2, J. Wuestenbergs2, W. Van de Voorde2, E. Cuypers1
1KU Leuven Toxicology and Pharmacology, Campus Gasthuisberg, Leuven, Belgium; 2KU Leuven, Imaging and Pathology Department, Division Forensic Biomedical Sciences, University of Leuven (KU Leuven), Campus Sint-Rafaël, Leuven, Belgium
P 174The performance of Immobilized Artificial Membrane (IAM) chromatography to model ecotoxicity of pesticides
F. Tsopelas1, C. Stergiopoulos1, L.A. Tsakanika, M. Ochsenkühn-Petropoulou1, A. Tsantili-Kakoulidou2
1Laboratory of Inorganic and Analytical Chemistry, School of Chemical Engineering, National Technical University of Athens, Greece; 2Department of Pharmaceutical Chemistry, School of Pharmacy, National and Kapodistrian University of Athens, Greece
P 175Simultaneous analysis of 20 unauthorized pharmaceutical compounds associated with treating senile diseases illegally adulterated in dietary supplements using UPLC-UV
S. Park, N.S. Kim, H.S. Choi, J.H. Kim, S.H. Moon, H. Kang
Division of Advanced Analysis, National Institute of Food and Drug Safety Evaluation
P 176Using MS/MS All with SWATH® acquisition for detection of fentanyl analogs and synthetic opioids in real hair samples with the SCIEX X500R QTOF system
D. Di Corcia, A. Salomone, E. Gerace, M. Vincenti
Centro Regionale Antidoping “A. Bertinaria”, Turin, Italy; Dipartimento di Chimica Analitica, Università degli Studi di Torino, Italy
P 177Organophosphate intoxication: Improvement of biological barriers penetration of AChE reactivators by encapsulation with cucurbit(7)uril
R. Andrýs1, A. Klusoňová1, M. Lísa1, J. Žďárová-Karasová2
1Department of Chemistry, Faculty of Science, University of Hradec Králové, Czech Republic; 2Department of Toxicology and Military Pharmacy, Faculty of Military Health Sciences Hradec Králové, University of Defence, Praha, Czech Republic
P 178Extraction and Quantification of Synthetic Cathinones From Human Urine using SOLA™ SPE and HILIC-MS/MS
M.R. Franklin, K. Woodmansey
Thermo Fisher Scientific, Tudor Road, Runcorn, United Kingdom

Applications - Metabolomics and lipidomics

P 179RAPID ANALYTICAL PLATFORMS FOR BIOFLUID PROFILING IN DISCOVERY METABOLOMICS
A.M. King1,2, L.G. Mullin3, I.D. Wilson4, P.D. Rainville3, R.S. Plumb3, L.A. Gethings1, G. Maker2, R. Trengove2
1Waters Corporation, Wilmslow, Cheshire, UK; 2Separations Science and Metabolomics laboratory, Murdoch
University, Murdoch, WA, Australia;
3Waters Corporation, Milford, USA; 4Computational and Systems Medicine, Department of Surgery and Cancer, Faculty of Medicine, Imperial College London, UK
P 180SPENT MEDIA MONITORING BY LIQUID CHROMATOGRAPHY COUPLED TO CHARGED AEROSOL DETECTOR (LC-CAD)
M.R. da Silva, I. Zaborowska, S. Carillo, J. Bones
NIBRT - National Institute for Bioprocessing Research & Training, Dublin, Ireland
P 181COMPLEMENTARY ANALYSIS OF POLAR AND NON-POLAR LIPIDS BY HEART-CUT TWO-DIMENSIONAL LIQUID CHROMATOGRAPHY COUPLED TO MASS SPECTROMETRY
C.M. Wienken, H. Hayen
Institute of Inorganic and Analytical Chemistry, University of Münster, Münster, Germany
P 182PHYTOCHEMICAL CHARACTERIZATION OF PROTEA PURE AND HYBRID CULTIVARS WITH THE AID OF LIQUID CHROMATOGRAPHY-ION MOBILITY MASS SPECTROMETRY (LC-IMMS) AND THE APPLICATION OF METABOLOMICS TOOLS IN DETERMINING POSSIBLE METABOLITES LINKED TO LEAF BLACKENING
K. Masike1, A. de Villiers2, M. Stander1,3
1Department of Biochemistry, University of Stellenbosch, Matieland, South Africa; 2Department of Chemistry and Polymer Science, University of Stellenbosch, Matieland, South Africa; 3Central Analytical Facility, Stellenbosch University, Matieland, South Africa
P 183CE-MS-BASED PLASMA METABOLOMICS FOR THE DISCOVERY OF BIOMARKERS FROM A MOUSE MODEL FOR ACUTE EPILEPTIC SEIZURES
K. Segers1,2, W. Zhang3, D. Mangelings1, T. Hankemeier3, D. De Bundel2, Y.V. Heyden1, I. Smolders2, R. Ramautar3, A. Van Eeckhaut2
1Department of Analytical Chemistry, Applied Chemometrics and Molecular Modelling, Vrije Universiteit Brussel, Belgium; 2Department of Pharmaceutical Chemistry, Drug Analysis and Drug Information, Center for Neurosciences (C4N), Vrije Universiteit Brussel, Belgium; 3Division of Systems Biomedicine and Pharmacology, Leiden Academic Centre for Drug Research (LACDR), Leiden University, The Netherlands
P 184HIGH-THROUGHPUT COMPREHENSIVE COVERAGE OF HYDROPHILIC AND HYDROPHOBIC METABOLITES IN BEER UTILIZING A DUAL SEPARATION/HIGH RESOLUTION ACCURATE MASS SPECTROMETRY SYSTEM
I. Ntai, M. Samonig, S. Samra, A. Souza, C. Paul, A. Huhmer
Thermo Fisher Scientific, San Jose, CA, USA; Thermo Fisher Scientific, Germering, Germany
P 185METABOLITE PROFILING OF YEAST CELLS BY MEANS OF CAPILLARY ION CHROMATOGRAPHY-MASS SPECTROMETRY
H. Schöttler, H. Hayen
Institute of Inorganic and Analytical Chemistry, University of Muenster, Germany
P 186GRAPHITIZED CARBON BLACK ENRICHMENT PROCEDURE FOR THE DETECTION OF PHOSPHOLIPIDS IN OLIVE OIL BY LIQUID CHROMATOGRAPHY-HIGH RESOLUTION MASS SPECTROMETRY
C.M. Montone, M. Antonelli, B. Benedetti, A. Cerrato, A. Laganà
Department of Chemistry, Sapienza Università di Roma, Italy
P 187THE METABONOMIC PROFILING OF CHICKEN EGGS DURING STORAGE USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY QUADRUPOLE TIME-OF-FLIGHT MASS SPECTROMETRY
A.E. Johnson, K.L. Sidwick, V.R. Pirgozliev, D.F. Thompson
PhD student, Chemical and Physical Sciences, Keele University, Newcastle-under-Lyme, Staffordshire
P 188QUANTIFICATION OF POLAR METABOLITES IN URINE USING AUTOMATED PARALLEL DERIVATIZATION AND LC-SWATH/MS
M.F. Cifuentes Girard1, D. Ruskic1, R. Picenoni2, G. Boehm2, G. Hopfgartner1
1Life Sciences Mass Spectrometry, Department of analytical and Inorganic Chemistry, University of Geneva, Switzerland; 2CTC Analytics AG, Zwingen, Switzerland
P 189Evaluation of antidepressant-like activity of kiwifruit (Actinidia deliciosa) extract on mouse models of depression
C.M. Marzo, M. Bianconi, C. Chiamulera, F. Guzzo
University of Verona, Department of Biotechnology, Verona, Italy; University of Verona, Department of Biotechnology, Verona, Italy; University of Verona, Department of Diagnostic and Public Health, Verona, Italy; University of Verona, Department of Biotechnology, Verona, Italy
P 190METABOLOMICS-ASSISTED METABOLITE PROFILING OF SEONGSANAMIDE A IN HUMAN LIVER MICROSOMES
K.H. Liu1, Z. Wu1, G.H. Lee1, H. Choi2
1BK21 Plus KNU Multi-Omics based Creative Drug Research Team, College of Pharmacy and Research Institute of Pharmaceutical Sciences, Kyungpook National University, Korea; 2College of Pharmacy, Yeungnam University, Korea
P 191THE DETECTION OF REMAINS IN WATER - SMALL MOLECULE PROFILING
M. Williams, F. Drijfhout
Keele University
P 192Comprehensive UHPLC-MS/MS lipidomics profiling to study effects of betulin on keratinocytes
C. Calderón, I. Merfort, M. Lämmerhofer
Institute of Pharmaceutical Sciences, Pharmaceutical (Bio-)Analysis, University of Tübingen, Germany; Institute of Pharmaceutical Sciences, Department of Pharmaceutical Biology and Biotechnology, University of Freiburg, Germany; Institute of Pharmaceutical Sciences, Pharmaceutical (Bio-)Analysis, University of Tübingen, Germany
P 193Lipidomic analysis and comparison of human body fluids and exosomes by various MS techniques
O. Peterka1, R. Jirásko1, M. Chocholoušková1, D. Wolrab1, L. Kuchař2, M. Holčapek1
1University of Pardubice, Faculty of Chemical Technology, Department of Analytical Chemistry, Pardubice, Czech Republic; 2Research Unit for Rare Diseases, Department of Pediatrics and Adolescent Medicine, First Faculty of Medicine, Charles University and General University Hospital in Prague, Czech Republic
P 194Challenges in the structural elucidation of Gram-negative bacterial lipid A molecules applying HPLC-MS/MS
Á. Dörnyei1, V. Sándor2, A. Kilár2, B. Kocsis3, F. Kilár1,2
1Department of Analytical and Environmental Chemistry, and Szentágothai Research Centre, University of Pécs, Hungary; 2Institute of Bioanalysis, Medical School, University of Pecs, Hungary; 3Department of Medical Microbiology and Immunology, Medical School, University of Pécs, Hungary
P 195Rapid analysis of selected neurotransmitters using hydrophilic interaction liquid chromatography
M. Alsaeedi1,2, A.M. Hogan1,2, J.D. Glennon1,2
1Innovative Chromatography Group, Irish Separation Science Cluster (ISSC); 2School of Chemistry and the Analytical, Biological and Chemistry Research Facility (ABCRF), University College Cork, Ireland
P 196Fecal oxo-bile acid composition evaluated by HPLC-ESI-MS/MS in mice model of experimental ulcerative colitis
E. Porru1, J. Fiori1, N. McGinley2, S. Keely2, A. Roda1
1Department of Chemistry “G. Ciamician”, Alma Mater Studiorum University of Bologna, Italy; 2Royal College of Surgeons in Ireland, Education and Research Centre, Beaumont Hospital, Dublin, Ireland
P 197Functional characterization of the human gut microbiota
V. Vidová, G. Dovrtělová, K. Coufalíková, E. Stuchlíková, A.-C. Aust, J. Klanová, Z. Spáčil
RECETOX, Faculty of Science, Masaryk University, Brno, Czech Republic
P 198Lipidomic changes in patients with nonalcoholic fatty liver disease (NAFLD) depending on histological severity
Y. Jung1,2, W. Kim3, G.S. Hwang1,4
1Integrated Metabolomics Research Group, Western Seoul Center, Korea Basic Science Institute, Seoul, Republic of Korea; 2Department of Life Science, Ewha Womans University, Seoul, Republic of Korea; 3Division of Gastroenterology and Hepatology, Department of Internal Medicine, Seoul National University College of Medicine, Seoul Metropolitan Government Boramae Medical Center, Seoul, Republic of Korea; 4Department of Chemistry and Nano Science, Ewha Womans University, Seoul, Republic of Korea
P 199Towards greater metabolite coverage – use of parallel LC-MS to cover analysis of metabolites and lipids in a single instrument setup
M. Witting1,2, G. Greco3, D. Hemmler1, F. Steiner3, P. Schmitt-Kopplin1,2
1Research Unit Analytical BioGeoChemistry, Helmholtz Zentrum München, Neuherberg, Germany; 2Chair of Analytical Food Chemistry, Technische Universität München, Freising, Germany; 3Thermo Fisher Scientific, Germering, Germany
P 200Development and validation of an LC-MS/MS method for determination of vitamin B-group and some its derivatives in whole blood
D. Kahoun1, P. Fojtíková1, E. Nováková2, V. Hypša2
1Institute of Chemistry, Faculty of Science, University of South Bohemia in Ceske Budejovice, Ceske Budejovice, Czech Republic; 2Institute of Parasitology, Faculty of Science, University of South Bohemia in Ceske Budejovice, Ceske Budejovice, Czech Republic

Applications - Natural products

P 201INVESTIGATION OF FREE AMINO ACID PROFILE AND ANTIOXIDANT EFFECTS OF EGG CHALAZA AFTER ENZYMATIC HYDROLYZATION
S.Y. Wang1, J.K. Tseng2,3, C.J. Chan1, Y.L. Lin1, J.W. Chen1,4, D.J. Yang5, Y.C. Chen1
1Department of Animal Science and Technology, National Taiwan University, Taipei, Taiwan; 2Department of Optometry, Asia University, Taichung, Taiwan; 3Department of Medical Research, China Medical University Hospital, China Medical University, Taichung, Taiwan; 4Poultry Industry Section, Department of Animal Industry, Council of Agriculture, Executive Yuan, Taipei, Taiwan; 5Institute of Food Safety and Health Risk Assessment, National Yang-Ming University, Taipei, Taiwan
P 202ANALYSIS OF STEROID HORMONES IN SHELLED EGGS FROM LAYER BREEDS COMMON TO TAIWAN BY LIQUID CHROMATOGRAPHY-TANDEM MASS SPECTROMETRY
S.Y. Wang1, I.C. Li2, W.Y. Yang3, C.H. Chou2, Y.C. Chen1, S.L. Kuo4
1Department of Animal Science and Technology, National Taiwan University, Taipei, Taiwan; 2Zoonoses Research Center and School of Veterinary Medicine, National Taiwan University, Taipei, Taiwan; 3Department of Basic Science, College of Veterinary Medicine, Mississippi State University, USA; 4Technical Service Center, National Animal Industry Foundation, Pingtung, Taiwan
P 203INHIBITORY EFFECTS OF PATENT ACTIVE WATER ON PARTICULATE MATTER 2.5 UM (PM2.5)-INDUCED LESION IN ENDOTHELIAL CELLS
S.L. Peng1, Y.W. Liu2, C.J. Weng2
1Institute of Medicine. Chung Shan Medical University, Taichung, Taiwan; 2Master Degree Program of Applied Living Science in Department of Living Services Industry, Tainan University of Technology, Taiwan
P 204DEVELOPMENT OF NEW APPROACHES FOR DETERMINATION AND IDENTIFICATION OF COMPONENTS FROM PLANT MATERIALS USING HPLC-MS DATA
P. Turova, I. Rodin, A. Stavrianidi
Faculty of Chemistry, Lomonosov Moscow State University, Moscow, Russia
P 205OBTAINING AND ANALYSING TROPICAL FLOWER COLORANTS FOR THEIR USE IN TEMPERA PAINTINGS
E.E. Stashenko, L. Sierra, R. González, J. Mejía, Y. Córdoba, J.R. Martínez
Chromatography and Mass Spectrometry Research Centre, CROM-MASS-CENIVAM. Universidad Industrial de Santander, Bucaramanga, Colombia
P 206NEW TOOLS FOR OAK SPECIES IDENTIFICATION
G. Buche1, C. Colas1, L. Fougère1, T. Giordanengo2, E. Destandau1
1Univ. Orléans, CNRS, ICOA, UMR 7311, Orléans, France; 2R&D Tonnellerie Radoux, Jonzac, France
P 207DETERMINATION OF PHYTOBIOTICS BY REVERSED-PHASE ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY FROM COMPLEX MATRIX
J. Rico-Ruiz, A. Casarín-Valverde, M. Olvera-Garcia, H. Leyva-Jimenez, L. Gómez-Osorio, G. Villar-Patiño
Euro-Nutec, México
P 208Identification of some polyphenols from aerial parts extracts of Daucus carota by LCMS
H. Kadri1, S.E. Djilani1, A. Djilani1, A. Dicko2
1LSBO, Badji Mokhtar University, Annaba, Algeria; 2LCME, Lorraine University, Metz, France
P 209SENSITIVE AND SELECTIVE ANALYSIS OF WOOD SUGARS AND URONIC ACIDS FOR BIOFUEL RESEARCH WITH ELECTROCHEMICAL DETECTION
K. Folmert, K. Monks
KNAUER Wissenschaftliche Geräte GmbH, Berlin, Germany
P 210Development of HPLC/MS/MS method for analysis of biologically active compounds in Tropaeolum majus
L. Česlová, T. Šalomounová
Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Czech Republic
P 211Comparative study of four extraction methods of Lawsonia inermis harvested from two different regions in Morocco and their HPLC-MS analysis
F.Z. Alem, G.S. Ananthabhotla, A.L.Oliveira, Z. Charrouf, B. Rhourri-Frih
Faculty of Pharmacy-University of Bordeaux CGFB - CBMN - UMR 5248, Bordeaux, France; Universidade de São Paulo, Pirassununga, Brazil; Faculty of Sciences, Mohammed V, Rabat, Morocco; Narayana Pharmacy College, Jawaharlal Nehru Technological University Anantapur, India
P 212Chemical composition determined by UHPLC-ESI-Orbitrap-MS and evaluation of the antioxidant potential Lippia origanoides extracts before and after plant hydrodistillation
J.J. Mejía Medina1, Y. Córdoba Campo1, L.J. Sierra1, A.J. Arias1, A. Kumara2, J.R. Martínez1, E.E. Stashenko1
1Research Center for Chromatography and Mass Spectrometry, CIBIMOL-CENIVAM, Universidad Industrial de Santander, Colombia; 2Department of Chemistry, Hemwati Nandan Bahuguna Garhwal University, Uttarakhand, India
P 213ionic liquid-based surfactants for the extraction of natural compounds from Vitis Vinifera leaves
G. Mastellone1,2, I. Pacheco-Fernández2, C. Cagliero1, V. Pino2,3
1Dipartimento di Scienza e Tecnologia del Farmaco, Universtà degli Studi di Torino, Italy; 2Departamento de Química, Unidad Departamental de Química Analítica, Universidad de La Laguna, Tenerife, Spain; 3University Institute of Tropical Diseases and Public Health, Universidad de La Laguna, Tenerife, Spain
P 214The simultaneous determination of six furocoumarin markers in citrus oil by UPLC coupled with UV and MS detection
Z. Li, P. Hong, P. McConville
Waters
P 215HPLC-UV Method Development for Baseline Resolution of 17 Cannabinoids
M. Wilcox, E. Franklin, S. Anderson
Regis Technologies, Inc, Morton Grove, Illinois, USA
P 216Comprehensive two-dimensional analysis of saponins from Quillaja Saponaria: comparison of RPLCxRPLC and HILICxRPLC modes
N. Biedermann1,2, S. Nuccio2, R. de Vaumas2, K. Faure1
1Institut des Sciences Analytiques, CNRS, Université Lyon, Villeurbanne, France; 2Extrasynthèse, Genay, France
P 217CHARACTERIZATION OF A STANDARDIZED EXTRACT OF FRESH CURCUMA LONGA RHIZOMES
V. Bardot1, A. Leblanc1, L. Berthomier2, I. Ripoche2, M. Leremboure2, Pierre Chalard2, Michel Dubourdeaux1
1Groupe Pileje, Paris, France; 2Université Clermont Auvergne, CNRS, SIGMA Clermont, Clermont-Ferrand, France
P 218Use of bioactives from agrifood by-products in topical cosmesic hydrogels
C. Stevanin1, E. Esposito1, R. Cortesi1, M. Sguizzato1, R. Voltan2, N. Marchetti1
1Department of chemical and pharmacologicall sciences, University of Ferrara, Italy; 2Department of morphology, surgery and medicine

Applications - Other

P 219DISPOSABLE ZIRCONIUM-GOLD ELECTRODE FOR SENSITIVE ELECTROCHEMICAL DETECTION IN HPLC
M.A. Islam1, P. Mahbub1,2, M. Macka1,3,4, B. Paull1,5
1Australian Centre for Research on Separation Science (ACROSS), School of Natural Sciences, University of Tasmania, Hobart, Australia; 2Institute for Sustainable Industries and Liveable Cities, Victoria University, Melbourne, Australia; 3Department of Chemistry and Biochemistry, Mendel University in Brno, Czech Republic; 4Central European Institute of Technology, Brno University of Technology, Brno, Czech Republic; 5ARC Training Centre for Portable Analytical Separation Technologies (ASTech), School of Natural Sciences, University of Tasmania, Hobart, Australia
P 220Analysis and evaluation of Monosaccharide composition and by-products in various cellulose nanofibers(CNFs) by HPLC
A. Morita, Y. Toyota, M. Kawashima, Y. Hirao
Shimadzu Corporation
P 221HPLC COLUMNS WITH UNIQUE SELECTIVITY FOR NUCLEIC ACIDS
A. Levine, T. Ono, T. Hirose, D. Keck
Nacalai USA, San Diego, USA; Nacalai USA, San Diego, USA; Nacalai Tesque Inc., Kyoto, Japan; Nacalai Tesque Inc., Kyoto, Japan
P 222Chromatographic behaviour of newly prepared double-labeled oligonucleotide probes containing azaphthalocyanines dye and different fluorophores
R. Kučera, A. Sčensná, M. Miletín, P. Zimčík
Charles University, Faculty of Pharmacy in Hradec Králové, Dept. of Pharmaceutical Chemistry and Pharmaceutical Analysis, Czech Republic
P 223Bioactive polymer lubricant unconsciously administered into the body
K. Naumoska1, U. Jug1,2, V. Metličar1,2, I Vovk1
1Department of Food Chemistry, National Institute of Chemistry, Ljubljana, Slovenia; 2Faculty of Chemistry and Chemical Technology, University of Ljubljana, Slovenia
P 224Polymeric lubricant, an usually overlooked interference in analytical studies and bioassays
U. Jug1,2, K. Naumoska1,3, V. Metličar1,2, A. Schink3, D. Makuc4, I. Vovk1, J. Plavec1,4,5, K Lucas3
1Department of Food Chemistry, National Institute of Chemistry, Ljubljana, Slovenia; 2Faculty of Chemistry and Chemical Technology, University of Ljubljana, Ljubljana, Slovenia; 3Multiphase Chemistry Department, Max Planck Institute for Chemistry, Mainz, Germany; 4Slovenian NMR Centre, National Institute of Chemistry, Ljubljana, Slovenia; 5EN-FIST Centre of Excellence, Ljubljana, Slovenia
P 225Towards the identification of the lichen species in historical orchil dyes by HPLC-MS/MS
F. Gosetti1, E. Calà1, M. Benzi1, M. Gulmini2, A. Idone2, I. Serafini3,4, A. Ciccola4, R. Curini3, I. Whitworth5, M. Aceto1
1Department of Science and Technological Innovation, University of Piemonte Orientale, Alessandria, Italy; 2Department of Chemistry, University of Torino, Italy; 3Department of Chemistry, University of Roma "La Sapienza", Italy; 4Department of Chemistry and Pharmaceutical Technologies, University of Roma "La Sapienza", Italy
P 226Optimising lipid separation for cultural heritage applications
J. Keute, T. Devièse
Research Lab for Archaeology and the History of Art, University of Oxford, United Kingdom
P 227Determination of Fluoride in LiPF6-based Lithium Ion Battery Electrolytes by Column-Switching Ion Chromatography
V. Kraft, P. Jochems, R. Ludwig, G.J. Schad
Shimadzu Europa GmbH, Duisburg, Germany
P 228Fast and Easy Injection of Large Sample Volumes in Preparative HPLC
F. Rieck
Agilent Technologies, Inc., Waldbronn, Germany

Applications - Proteomics